scholarly journals Inhibitory Effects of Hot Water Extract of the Stevia Stem on the Contractile Response of the Smooth Muscle of the Guinea Pig Ileum

2006 ◽  
Vol 70 (2) ◽  
pp. 489-494 ◽  
Author(s):  
Kazuhiro SHIOZAKI ◽  
Akiko FUJII ◽  
Toshiki NAKANO ◽  
Toshiyasu YAMAGUCHI ◽  
Minoru SATO
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Contractile Response ◽  
Water Extract ◽  
Hot Water ◽  
Inhibitory Effects ◽  
Guinea Pig Ileum ◽  
Hot Water Extract

scholarly journals Allelopathic Potential of Papaver Somniferum L. on Seed Germination Against Three Different Varities of Zea Mays

2021 ◽  
Author(s):  
Muntaha Tul Sidra ◽  
SAYEDA SARAH MUBARAK ◽  
FATIMA KHAN SHERWANI ◽  
NAZMA AZEEM
Keyword(s):  
Zea Mays ◽  
Aqueous Extract ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Papaver Somniferum ◽  
Hot Water ◽  
Allelopathic Effect ◽  
Inhibitory Effects ◽  
Allelopathic Potential ◽  
Hot Water Extract

Abstract Aims To evaluate the allelopathic effect of Papaver somniferum against the 3 varieties of Zea mays seeds cv. Azam, Pahari and Iqbal. Methods Aqueous extracts were made by soaking the powder of dried leaves of Papaver somniferum. For the aqueous extract treatment, the extract was soaked in distilled water for 24 and 48 hours separately. For mulching treatment, the seeds were sowed in soil and sprinkled powder of leaves. For litter, the petri dishes were grounded with randomly cut pieces of filter paper wetted with extract. For hot water treatment the water was boiled for 5 minutes. The powder was then mixed in boil water. Results Azam got effected in 24h extract in aqueous extract treatment while Pahari in 48h treatment. In mulching all the three varieties got inhibited by extract. Azam showed resistance toward the inhibitory effect of extract in litter experiments. Iqbal was affected in hot water extract. Conclusion It is concluded that the extracts obtained from Papaver somniferum showed inhibitory effects on all the three varieties except few exceptions of maize. The plumule and radical growth got affected the most.

scholarly journals Phytochemical Evaluation of Lythrum Salicaria Extracts and Their Effects on Guinea-Pig Ileum

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Tímea Bencsik ◽  
Loránd Barthó ◽  
Viktor Sándor ◽  
Nóra Papp ◽  
Rita Benkó ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Lythrum Salicaria ◽  
Dependent Manner ◽  
Guinea Pig Ileum ◽  
Concentration Dependent Manner ◽  
Ethanol In Water

n-Hexane, chloroform, ethyl acetate and 50% ethanol in water extracts prepared from the air-dried flowering parts of Lythrum salicaria L. were tested for in vitro pharmacological properties on Guinea-pig ileum, which is suitable for detecting a whole range of neuronal and smooth muscle effects. UHPLC-MS was used to evaluate polyphenol components of the extracts. In the ileum, the most prominent response (46.4% related to 0.5 μM histamine) of the extracts causing smooth muscle contractions were triggered by the 50% ethanol in water extract in a concentration-dependent manner. Atropine, indomethacin and PPADS plus suramin significantly reduced the contractile response caused by this extract. The strongest inhibition was due to atropine. The results suggest that L. salicaria extracts have a moderate muscarinic receptor agonist effect in Guinea-pig ileum and that prostanoids and purinoceptor mechanisms are involved to some extent. Therefore diluted extracts of L. salicaria p.o. could be used as a mild stimulant of gastrointestinal motility. The 50% ethanol in water extract was rich in polyphenols. n-Hexane, chloroform and ethyl acetate extracts failed to contain catechin, caffeic acid, quercetin-3-D-galactoside and rutin, but they all showed spasmogenic effects, and, therefore we do not think that these compounds could be involved in the spasmogenic activity.

Effect of CB1 Ligands on Neurogenic and Myogenic Contractile Responses in the Guinea-Pig Ileum

Pharmacology ◽  
2018 ◽  
Vol 101 (5-6) ◽  
pp. 330-336 ◽  
Author(s):  
Josef Donnerer ◽  
Ingrid Liebmann
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Cannabinoid Receptor ◽  
Allyl Isothiocyanate ◽  
Mustard Oil ◽  
Inhibitory Effects ◽  
Guinea Pig Ileum ◽  
Sr 141716A ◽  
Smooth Muscle Contractions ◽  
Evoked Contractions

This study aimed at investigating whether the synthetic cannabinoid receptor agonist (+)-WIN 55212-2 has neurogenic and myogenic relaxant effects on the longitudinal muscle-myenteric plexus (LMMP) strip of the guinea-pig ileum. (+)-WIN 55212-2, 1–1,000 nmol/L, concentration-dependently inhibited both the electrical stimulation-induced cholinergic twitch responses as well as the myogenic smooth muscle contractions in the LMMP preparation. SR-141716A (rimonabant) 1–1,000 nmol/L, the cannabinoid CB1 receptor antagonist, being without effect on its own, antagonized the (+)-WIN 55212-2-induced effects. The allyl isothiocyanate (mustard oil, 100 µmol/L) induced a relaxant effect in the guinea-pig ileum, which can be regarded as neurogenic and myogenic, was augmented by (+)-WIN 55212-2, and inhibited by SR-141716A. (+)-WIN 55212-2 only moderately modified the 60 mmol/L KCl-evoked contractions. These results provide functional evidence that the CB1 agonist (+)-WIN 55212-2-induced inhibitory effects in the guinea-pig ileum are exerted both at the neuronal as well as at the intestinal smooth muscle cell level.

scholarly journals Chemical Constituents of the Leaves of Campanula takesimana (Korean Bellflower) and Their Inhibitory Effects on LPS-induced PGE2 Production

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1232
Author(s):  
Yutong Qi ◽  
Se-In Choi ◽  
So-Ri Son ◽  
Hee-Soo Han ◽  
Hye Shin Ahn ◽  
...  
Keyword(s):  
Chemical Constituents ◽  
Water Extract ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Pge2 Production ◽  
Raw 264.7 Cells ◽  
Inhibitory Effects ◽  
Spectrometric Data ◽  
Traditional Medicines ◽  
Hot Water Extract

Campanula takesimana Nakai (Campanulaceae; Korean bellflower) is one of the endemic herbs of Korea. The plant has been used as traditional medicines for treating asthma, tonsillitis, and sore throat in Korea. A hot water extract of the leaves of C. takesimana exhibited a significant inhibitory effect on lipopolysaccharide (LPS)-stimulated prostaglandin E2 (PGE2) production. Repetitive chromatographic separation of the hot water extract led to the isolation of three new neolignan glucosides, campanulalignans A–C (1–3), with 15 known compounds (4–18). The structures of new compounds 1–3 were elucidated by analyzing nuclear magnetic resonance (NMR) spectroscopic data, along with high resolution quadrupole time of flight mass (HR-Q-TOF-MS) spectrometric data. Among the isolates, simplidin (7), 5-hydroxyconiferaldehyde (11), icariside F2 (12), benzyl-α-l-arabinopyranosyl-(1″→6′)-β-d-glucopyranoside (13), and kaempferol 3-O-β-d-apiosyl (1→2)-β-d-glucopyranoside (15) were isolated from the Campanulaceae family for the first time. The isolates (1, 2, and 4–18) were assessed for their anti-inflammatory effects on LPS-stimulated PGE2 production on RAW 264.7 cells. 7R,8S-Dihydrodehydrodiconiferyl alcohol (5), 3′,4-O-dimethylcedrusin 9-O-β-glucopyranoside (6), pinoresinol di-O-β-d-glucoside (8), ferulic acid (10), 5-hydroxyconiferaldehyde (11), and quercetin (18) showed significant inhibitory effects on LPS-stimulated PGE2 production.

K transport and mechanical responses of isolated longitudinal smooth muscle from guinea pig ileum

1961 ◽  
Vol 200 (4) ◽  
pp. 789-793 ◽  
Author(s):  
George B. Weiss ◽  
Robert E. Coalson ◽  
Leon Hurwitz
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Contractile Response ◽  
Muscle Tone ◽  
Muscle Layer ◽  
Smooth Muscle Contraction ◽  
Potassium Ion ◽  
Guinea Pig Ileum ◽  
Potassium Efflux ◽  
Longitudinal Smooth Muscle

The longitudinal smooth muscle layer of the guinea pig ileum was isolated in order to investigate its contractile responses and unidirectional K42 fluxes. Pilocarpine (7.5 x 10–6 m), acetylcholine (6.6 x 10–6 m), and a modified Tyrode's solution in which potassium ion was substituted for almost all the sodium ion were employed as excitatory agents. Cocaine (8.5 x 10–4 m) and a calcium-free Tyrode's solution served as inhibitory agents. Smooth muscle tone and potassium efflux of this relatively pure tissue were both increased by all three excitatory substances. Moreover, acetylcholine and pilocarpine produced a decrease in the influx of potassium ion. Bathing the tissue in a calcium-free medium for 1 hour before introducing pilocarpine to the muscle bath eliminated the contractile response that this drug ordinarily produces, but did not diminish appreciably the increase in K42 efflux. These observations are qualitatively similar to results previously obtained in analogous experiments on isolated whole ileum. In addition, cocaine (8.5 x 10–4 m) was found to block the contractile response and about three-quarters of the enhanced K42 efflux elicited by the isotonic potassium solution. It is presumed that cocaine acting at the membrane impedes ion fluxes important for smooth muscle contraction.

Amphibian toxins from the skin of Bufo regularis have inhibitory effect on electrically invoked contractile response and bimodal effects on tone of longitudinal muscle of guinea pig ileum

2019 ◽  
Vol 34 (1) ◽  
pp. 9-22
Author(s):  
Tesfaye Tolessa
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
High Performance ◽  
Longitudinal Muscle ◽  
Contractile Response ◽  
Inhibitory Effect ◽  
Muscle Strip ◽  
Organ Bath ◽  
Guinea Pig Ileum ◽  
Longitudinal Smooth Muscle

The skins of some amphibians contain potentially bioactive principles that may have pharmaceutical, medicinal, toxicological or chemical importance. In addition, such active principles can be used as tools in biomedical research. The present study aims at isolating and purifying bioactive principles from the skin of Bufo regularis and studying their effect on isolated longitudinal smooth muscle strip of guinea pig ileum. High performance liquid chromatography (HPLC) was used to isolate toad toxins. The effects of crude, semi-purified and purified extracts were tested on longitudinal smooth muscle of guinea pig ileum using organ bath method. Effect of the toxins was studied on electrically-induced contractile response and the basal tone of the longitudinal muscle strip. HPLC purification resulted in four different bioactive components with a λmax UV absorbance pattern of around 295 nm. When tested on guinea pig ileum they had persistent inhibitory effect on the electrically-induced contractile responses. The pattern of effect was initial excitatory followed by long lasting inhibitory effect on tone of longitudinal muscle. The HPLC eluate at 79th min in methanol preparative run corresponding to the eluate at 40th min in the acetonitrile run had the maximum bioactivity. Hence, it was concluded that the skin of B. regularis contains four different components which vary in their potency on isolated smooth muscle strip of guinea pig ileum.Keywords: Bufo regularis, organ bath, longitudinal muscle of ileum, toad toxin, electrical field stimulation

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