Theanine Production by Coupled Fermentation with Energy Transfer EmployingPseudomonas taetrolensY-30 Glutamine Synthetase and Baker’s Yeast Cells

2005 ◽  
Vol 69 (4) ◽  
pp. 784-789 ◽  
Author(s):  
Sachiko YAMAMOTO ◽  
Mamoru WAKAYAMA ◽  
Takashi TACHIKI
Keyword(s):  
Energy Transfer ◽  
Glutamine Synthetase ◽  
Baker’S Yeast ◽  
Yeast Cells ◽  
Baker's Yeast

Characteristics and Efficiency of Glutamine Production by Coupling of a Bacterial Glutamine Synthetase Reaction with the Alcoholic Fermentation System of Baker’s Yeast

1998 ◽  
Vol 64 (8) ◽  
pp. 2952-2957 ◽  
Author(s):  
Shinji Wakisaka ◽  
Yoshifumi Ohshima ◽  
Masahiro Ogawa ◽  
Tatsurokuro Tochikura ◽  
Takashi Tachiki
Keyword(s):  
Energy Source ◽  
Glutamine Synthetase ◽  
Inorganic Phosphate ◽  
Baker’S Yeast ◽  
Yeast Cells ◽  
Yeast Fermentation ◽  
Baker's Yeast ◽  
Futile Cycle ◽  
Atp Regeneration ◽  
Glutamine Production

ABSTRACT Glutamine production with bacterial glutamine synthetase (GS) and the sugar-fermenting system of baker’s yeast for ATP regeneration was investigated by determining the product yield obtained with the energy source for ATP regeneration (i.e., glucose) for yeast fermentation. Fructose 1,6-bisphosphate was accumulated temporarily prior to the formation of glutamine in mixtures which consisted of dried yeast cells, GS, their substrate (glucose and glutamate and ammonia), inorganic phosphate, and cofactors. By an increase in the amounts of GS and inorganic phosphate, the amounts of glutamine formed increased to 19 to 54 g/liter, with a yield increase of 69 to 72% based on the energy source (glucose) for ATP regeneration. The analyses of sugar fermentation of the yeast in the glutamine-producing mixtures suggested that the apparent hydrolysis of ATP by a futile cycle(s) at the early stage of glycolysis in the yeast cells reduces the efficiency of ATP utilization. Inorganic phosphate inhibits phosphatase(s) and thus improves glutamine yield. However, the analyses of GS activity in the glutamine-producing mixtures suggested that the higher concentration of inorganic phosphate as well as the limited amount of ATP-ADP caused the low reactivity of GS in the glutamine-producing mixtures. A result suggestive of improved glutamine yield under the conditions with lower concentrations of inorganic phosphate was obtained by using a yeast mutant strain that had low assimilating ability for glycerol and ethanol. In the mutant, the activity of the enzymes involved in gluconeogenesis, especially fructose 1,6-bisphosphatase, was lower than that in the wild-type strain.

Purification and Properties of a Thermo-labile Antigen from Baker’s Yeast Cells

1981 ◽  
Vol 45 (12) ◽  
pp. 2713-2721
Author(s):  
Youichi Tamai ◽  
Hiroshi Shinmoto ◽  
Masayoshi Takakuwa
Keyword(s):  
Baker’S Yeast ◽  
Yeast Cells ◽  
Baker's Yeast ◽  
Purification And Properties

A greener bioreduction using baker’s yeast cells in supercritical carbon dioxide and glycerol system

2019 ◽  
Vol 143 ◽  
pp. 330-335
Author(s):  
Jonas Daci da Silva Serres ◽  
Pamela Taisline Bandeira ◽  
Paloma Cabral Zappani ◽  
Leandro Piovan ◽  
Marcos Lúcio Corazza
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Baker’S Yeast ◽  
Yeast Cells ◽  
Baker's Yeast ◽  
Supercritical Carbon

scholarly journals Fractionation of yeast biomass II. Release of cytoplazmatic content of baker's yeast cells.

1988 ◽  
Vol 34 (8) ◽  
pp. 241-245 ◽  
Author(s):  
E. ŠTURDÍK ◽  
R. KOLLÁR ◽  
I. BERNÁT ◽  
M. MIKULÁŠOVÁ ◽  
J. FORSTHOFFER ◽  
...  
Keyword(s):  
Baker’S Yeast ◽  
Yeast Cells ◽  
Baker's Yeast ◽  
Yeast Biomass

Atomic Force Microscopy Study of Living Baker's Yeast Cells

2011 ◽  
Vol 4 (2) ◽  
pp. 368-376 ◽  
Author(s):  
L. Mikoliunaite ◽  
A. Makaraviciute ◽  
A. Suchodolskis ◽  
A. Ramanaviciene ◽  
Y. Oztekin ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Microscopy Study ◽  
Baker’S Yeast ◽  
Yeast Cells ◽  
Baker's Yeast ◽  
Atomic Force Microscopy Study ◽  
Force Microscopy ◽  
Atomic Force

Elastic properties of chemically modified baker's yeast cells studied by AFM

2011 ◽  
Vol 43 (13) ◽  
pp. 1636-1640 ◽  
Author(s):  
Arturas Suchodolskis ◽  
Vidmantas Feiza ◽  
Arunas Stirke ◽  
Ana Timonina ◽  
Almira Ramanaviciene ◽  
...  
Keyword(s):  
Elastic Properties ◽  
Baker’S Yeast ◽  
Yeast Cells ◽  
Baker's Yeast ◽  
Chemically Modified

scholarly journals Production of .GAMMA.-glutamylmethylamide and .GAMMA.-glutamylethylamide by coupling of baker's yeast preparations and bacterial glutamine synthetase.

1986 ◽  
Vol 32 (6) ◽  
pp. 545-548 ◽  
Author(s):  
TAKASHI TACHIKI ◽  
HIDEYUKI SUZUKI ◽  
SHINJI WAKISAKA ◽  
TOSHIHIRO YANO ◽  
TATSUROKURO TOCHIKURA
Keyword(s):  
Glutamine Synthetase ◽  
Baker’S Yeast ◽  
Baker's Yeast
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