scholarly journals Molecular Cloning of a Genomic DNA Encoding Yam Class IV Chitinase

2004 ◽  
Vol 68 (7) ◽  
pp. 1508-1517 ◽  
Author(s):  
Takuji MITSUNAGA ◽  
Minoru IWASE ◽  
Wimal UBHAYASEKERA ◽  
Sherry L. MOWBRAY ◽  
Daizo KOGA
Keyword(s):  
Molecular Cloning ◽  
Genomic Dna ◽  
Dna Encoding ◽  
Class Iv Chitinase ◽  
Class Iv

scholarly journals Molecular cloning and sequencing of genomic DNA encoding yeast vacuolar carboxypeptidase yscS

FEBS Letters ◽  
1991 ◽  
Vol 283 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Javier Bordallo ◽  
Carmen Bordallo ◽  
Gascón Santiago ◽  
Paz Suárez-Rendueles
Keyword(s):  
Molecular Cloning ◽  
Genomic Dna ◽  
Dna Encoding ◽  
Cloning And Sequencing

Insect immunity: molecular cloning, expression, and characterization of cDNAs and genomic DNA encoding three isoforms of insect defensin in Aedes aegypti

1999 ◽  
Vol 8 (1) ◽  
pp. 107-118 ◽  
Author(s):  
C. A. Lowenberger ◽  
C. T. Smartt ◽  
P. Bulet ◽  
M. T. Ferdig ◽  
D. W. Severson ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Molecular Cloning ◽  
Genomic Dna ◽  
Insect Immunity ◽  
Dna Encoding ◽  
Insect Defensin

Molecular Cloning of a Genomic DNA Encoding the Potato β-1,3-Glucanase

1999 ◽  
Vol 155 (4-5) ◽  
pp. 520-526
Author(s):  
Tae-Ho Kwon ◽  
Tae-Geum Kim ◽  
Dae-Hyuk Kim ◽  
Moon-Sik Yang
Keyword(s):  
Molecular Cloning ◽  
Genomic Dna ◽  
Dna Encoding

MOLECULAR CLONING AND CHARACTERIZATION OF ADENYLOSUCCINATE LYASE cDNA AND GENOMIC DNA IN GRASS CARP

2010 ◽  
Vol 33 (6) ◽  
pp. 1105-1111
Author(s):  
Gang QU ◽  
Ji-Rui GU ◽  
Wen-Bo GU ◽  
Wen-Li ZHU ◽  
Jiang WU ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Grass Carp ◽  
Genomic Dna ◽  
Adenylosuccinate Lyase

MOLECULAR CLONING AND CHARACTERIZATION OF RAT BRAIN C-DNA ENCODING THE NPY-1 RECEPTOR.

1992 ◽  
Vol 15 ◽  
pp. 73B
Author(s):  
C. Eva ◽  
J. Krause ◽  
P. H. Seeburg ◽  
R. Sprengel
Keyword(s):  
Rat Brain ◽  
Molecular Cloning ◽  
Dna Encoding

scholarly journals Biochemical Analysis of Lpt3, a Protein Responsible forPhosphoethanolamine Addition to Lipooligosaccharide ofPathogenic Neisseria

2006 ◽  
Vol 188 (3) ◽  
pp. 1039-1048 ◽  
Author(s):  
Ellen T. O'Connor ◽  
Andrzej Piekarowicz ◽  
Karen V. Swanson ◽  
J. McLeod Griffiss ◽  
Daniel C. Stein
Keyword(s):  
Genomic Dna ◽  
Biochemical Analysis ◽  
Expression Vector ◽  
Inner Core ◽  
Genomic Sequence ◽  
Mass Spectrometry Analysis ◽  
Dna Encoding ◽  
Spectrometry Analysis ◽  
Transposon Insertion ◽  
Genomic Dna Sequence

ABSTRACT The inner core of neisserial lipooligosaccharide (LOS) contains heptose residues that can be decorated by phosphoethanolamine (PEA). PEA modification of heptose II (HepII) can occur at the 3, 6, or 7 position(s). We used a genomic DNA sequence of lpt3, derived from Neisseria meningitidis MC58, to search the genomic sequence of N. gonorrhoeae FA1090 and identified a homolog of lpt3 in N. gonorrhoeae. A PCR amplicon containing lpt3 was amplified from F62ΔLgtA, cloned, mutagenized, and inserted into the chromosome of N. gonorrhoeae strain F62ΔLgtA, producing strain F62ΔLgtAlpt3::Tn5. LOS isolated from this strain lost the ability to bind monoclonal antibody (MAb) 2-1-L8. Complementation of this mutation by genetic removal of the transposon insertion restored MAb 2-1-L8 binding. Mass spectrometry analysis of LOS isolated from the F62ΔLgtA indicated that this strain contained two PEA modifications on its LOS. F62ΔLgtAlpt3::Tn5 lacked a PEA modification on its LOS, a finding consistent with the hypothesis that lpt3 encodes a protein mediating PEA addition onto gonococcal LOS. The DNA encoding lpt3 was cloned into an expression vector and Lpt3 was purified. Purified Lpt3 was able to mediate the addition of PEA to LOS isolated from F62ΔLgtAlpt3::Tn5.

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