Radicicol Binding to Swo1/Hsp90 and Inhibition of Growth of Specific Temperature-sensitive Cell Cycle Mutants of Fission Yeast

2001 ◽  
Vol 65 (11) ◽  
pp. 2528-2534 ◽  
Author(s):  
Se Won KI ◽  
Koji KASAHARA ◽  
Ho Jeong KWON ◽  
Ken ISHIGAMI ◽  
Takeshi KITAHARA ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Temperature Sensitive ◽  
Sensitive Cell ◽  
Inhibition Of Growth ◽  
Specific Temperature

scholarly journals A temperature-sensitive cell-cycle mutant of mammalian cells, tsJT16, is defective in a function operating soon after growth stimulation.

1990 ◽  
Vol 15 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Tsuyoshi Takasuka ◽  
Jun Ninomiya-Tsuji ◽  
Mihoko Sakayama ◽  
Sadahiko Ishibashi ◽  
Toshinori Ide
Keyword(s):  
Cell Cycle ◽  
Mammalian Cells ◽  
Growth Stimulation ◽  
Temperature Sensitive ◽  
Sensitive Cell

The novel human protein serine/threonine phosphatase 6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, which are involved in cell cycle regulation

1996 ◽  
Vol 109 (12) ◽  
pp. 2865-2874 ◽  
Author(s):  
H. Bastians ◽  
H. Ponstingl
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Shape Control ◽  
Cell Cycle Regulation ◽  
Budding Yeast ◽  
Mitotic Checkpoint ◽  
Human Protein ◽  
Predicted Amino Acid Sequence ◽  
Temperature Sensitive ◽  
Cycle Regulation

We identified a novel human protein serine/threonine phosphatase cDNA, designated protein phosphatase 6 (PP6) by using a homology-based polymerase chain reaction. The predicted amino acid sequence indicates a 35 kDa protein showing high homology to other protein phosphatases including human PP2A (57%), human PP4 (59%), rat PPV (98%), Drosophila PPV (74%), Schizosaccharomyces pombe ppe1 (68%) and Saccharomyces cerevisiae Sit4p (61%). In human cells, three forms of PP6 mRNA were found with highest levels of expression in testis, heart and skeletal muscle. The PP6 protein was detected in lysates of human heart muscle and in bull testis. Complementation studies using a temperature sensitive mutant strain of S. cerevisiae SIT4, which is required for the G1 to S transition of the cell cycle, showed that PP6 can rescue the mutant growth arrest. In addition, a loss of function mutant of S. pombe ppe1, described as a gene interacting with the pim1/spi1 mitotic checkpoint and involved in cell shape control, can be complemented by expression of human PP6. These data indicate that human PP6 is a functional homologue of budding yeast Sit4p and fission yeast ppe1, implying a function of PP6 in cell cycle regulation.

scholarly journals A single-point mutation in HCF causes temperature-sensitive cell-cycle arrest and disrupts VP16 function.

1997 ◽  
Vol 11 (6) ◽  
pp. 726-737 ◽  
Author(s):  
H Goto ◽  
S Motomura ◽  
A C Wilson ◽  
R N Freiman ◽  
Y Nakabeppu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Point Mutation ◽  
Single Point ◽  
Single Point Mutation ◽  
Temperature Sensitive ◽  
Sensitive Cell ◽  
Cycle Arrest

scholarly journals Fission yeast cell morphogenesis: identification of new genes and analysis of their role during the cell cycle.

1995 ◽  
Vol 131 (6) ◽  
pp. 1529-1538 ◽  
Author(s):  
F Verde ◽  
J Mata ◽  
P Nurse
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Cell Polarity ◽  
Growth Direction ◽  
Temperature Sensitive ◽  
Cell Morphogenesis ◽  
Cell Cycle Genes ◽  
New Genes ◽  
Morphological Mutants ◽  
Mutant Phenotypes

To identify new genes involved in the control of cell morphogenesis in the fission yeast Schizosaccharomyces pombe we have visually screened for temperature-sensitive mutants that show defects in cell morphology. We have isolated and characterized 64 mutants defining 19 independent genes, 10 of which have not been previously described. One class of mutants, defining 12 orb genes, become round and show a complete loss of cell polarity. A second class of mutants exhibits branched or bent morphologies. These mutants show defects in either selection of the growth site, defining two tea genes, or in the maintenance of growth direction, defining five ban genes. Immunofluorescence analysis of these morphological mutants shows defects in the organization of the microtubule and actin cytoskeleton. These defects include shortened, bundled, and asymmetrically localized microtubules and enlarged and mislocalized actin patches. Analysis of the mutant phenotypes has allowed us to order the genes into four groups according to their function during the cell cycle: genes required for the maintenance of cell polarity throughout the cell cycle; genes necessary only for the reestablishment of cell polarity after mitosis and not for maintaining cell polarity once it is established; genes essential for the transition from monopolar to bipolar growth and genes that severe as 'polarity markers'.

Changes in RNA polymerase II in a cell cycle-specific temperature-sensitive mutant of hamster cells

1980 ◽  
Vol 103 (1) ◽  
pp. 97-103 ◽  
Author(s):  
Mara Rossini ◽  
Susan Baserga ◽  
C. H. Huang ◽  
C. James Ingles ◽  
Renato Baserga
Keyword(s):  
Cell Cycle ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
A Cell ◽  
Specific Temperature
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