The Expression of a Barley HνNAS1 Nicotianamine Synthase Gene Promoter-gus Fusion Gene in Transgenic Tobacco is Induced by Fe-deficiency in Roots

2001 ◽  
Vol 65 (7) ◽  
pp. 1692-1696 ◽  
Author(s):  
Kyoko HIGUCHI ◽  
Masaharu TANI ◽  
Hiromi NAKANISHI ◽  
Toshihiro YOSHIWARA ◽  
Fumiyuki GOTO ◽  
...  
1996 ◽  
Vol 31 (2) ◽  
pp. 307-322 ◽  
Author(s):  
Montserrat Capellades ◽  
Miguel Angel Torres ◽  
Ingo Bastisch ◽  
Virginia Stiefel ◽  
Florence Vignols ◽  
...  

2004 ◽  
Vol 21 (2) ◽  
pp. 113-118 ◽  
Author(s):  
Tomohide MAEDA ◽  
Yuriko WATAKABE ◽  
Seunghee SEO ◽  
Hisabumi TAKASE ◽  
Kazuyuki HIRATSUKA

2004 ◽  
Vol 32 (1) ◽  
pp. 33-53 ◽  
Author(s):  
JN Boustead ◽  
CC Martin ◽  
JK Oeser ◽  
CA Svitek ◽  
SI Hunter ◽  
...  

Glucose-6-phosphatase (G6Pase) catalyzes the final step in the gluconeogenic and glycogenolytic pathways, the hydrolysis of glucose-6-phosphate (G6P) to glucose and phosphate. This paper describes the identification and characterization of a cDNA and the gene encoding the mouse ubiquitously expressed G6Pase catalytic subunit-related protein (UGRP). The open reading frame of this UGRP cDNA encodes a protein (346 amino acids (aa); Mr 38,755) that shares 36% overall identity (56% similarity) with the mouse G6Pase catalytic subunit (357 aa; Mr 40,454). UGRP exhibits a similar predicted transmembrane topology and conservation of many of the catalytically important residues with the G6Pase catalytic subunit; however, unlike the G6Pase catalytic subunit, UGRP does not catalyze G6P hydrolysis and does not contain a carboxy-terminal di-lysine endoplasmic reticulum retention signal. UGRP mRNA was detected by RNA blot analysis in every mouse tissue examined with the highest expression in heart, brain, testis and kidney. Database analysis showed that the mouse UGRP gene is composed of six exons, spans approximately 4.2 kbp of genomic DNA and is located on chromosome 11 along with the G6Pase catalytic subunit gene. The UGRP gene transcription start sites were mapped by primer extension analysis, and the activity of the mouse UGRP gene promoter was analyzed using luciferase fusion gene constructs. In contrast to the G6Pase catalytic subunit gene promoter, the UGRP promoter was highly active in all cell lines examined.


2005 ◽  
Vol 272 (6) ◽  
pp. 690-699 ◽  
Author(s):  
Atsushi Morikami ◽  
Rie Matsunaga ◽  
Yoshimi Tanaka ◽  
Satomi Suzuki ◽  
Shoji Mano ◽  
...  

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