Induction of Oxidatively Modified Proteins in Skeletal Muscle by Electrical Stimulation and Its Suppression by Dietary Supplementation of (-)-Epigallocatechin Gallate

2000 ◽  
Vol 64 (5) ◽  
pp. 1004-1010 ◽  
Author(s):  
Takashi NAGASAWA ◽  
Hiromichi HAYASHI ◽  
Naoko FUJIMAKI ◽  
Naoyuki NISHIZAWA ◽  
David D. KITTS
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Epigallocatechin Gallate ◽  
Dietary Supplementation ◽  
Modified Proteins ◽  
Oxidatively Modified ◽  
Oxidatively Modified Proteins

scholarly journals EFFECTS OF MELATONIN ON OXIDANT AND ANTIOXIDANT STATUSIN THE BLOOD OF ALLOXAN DIABETIC RATS

2019 ◽  
pp. 5-10
Author(s):  
N. M. Luhinich ◽  
I. V. Gerush ◽  
N. P. Grygorieva
Keyword(s):  
Antioxidant Enzymes ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
Diabetic Rats ◽  
Sod Activity ◽  
Radical Oxidation ◽  
Modified Proteins ◽  
Oxidatively Modified ◽  
Ceruloplasmin Activity ◽  
Oxidatively Modified Proteins

Introduction. The experimental model of alloxane diabetes is quite common, which is often used to study the different aspects of pathogenesis and pathomorphology of diabetes. It is known that during the diabetes activation of free radical oxidation of biomolecules occurs as well as depletion of the antioxidant system. Melatonin can suppress reactive oxygen species and increases the activity of antioxidant enzymes. The aim of the study – to investigate the effect of melatonin on oxidant and antioxidant status in the blood of alloxan diabetic rats. Research Methods. Experiments were conducted on white outbred sexually mature male rats. In the blood plasma was determined content of oxidatively modified proteins, the ceruloplasmin activity; in the hemolisate of erythrocytes was determined content of TBA-reactive products, the measurement of superoxide dismutase (SOD) and catalase activities. Results and Discussion. The results of our studies showed that under conditions of alloxane diabetes, processes of free radical damage to biomolecules are intensified, as evidenced by the increase in the content of TBA-active products and oxidatively modified proteins in the blood of rats on 7 and 14 days of alloxane diabetes. The activity of catalase and ceruloplasmin in the blood of alloxan diabetic rats was lower than in the control group of animals but the SOD activity was significantly increased. The positive effect of melatonin is shown on 7 and 14 days and decrease the content of TBA-active products and oxidatively modified proteins blood of rats compared with untreated animals. Also, the administration of melatonin contributed to the normalization of the activity antioxidant enzymes of blood in rats with alloxane diabetes: increase of catalase activity in erythrocytes, ceruloplasmin activity and decrease SOD activity compared with untreated animals. Conclusions. The introduction of exogenous melatonin in rats with alloxane diabetes in a dose of 10 mg/kg daily for 7 and 14 days cause a pronounced antioxidant effect, reducing free radical oxidation and normalizing the activity of enzymes of antioxidant defense in the blood alloxan diabetic rats.

scholarly journals Oxidatively Modified Proteins in the Serous Subtype of Ovarian Carcinoma

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sharifeh Mehrabi ◽  
Edward E. Partridge ◽  
William Seffens ◽  
Xuebiao Yao ◽  
Felix O. Aikhionbare
Keyword(s):  
African Americans ◽  
Ovarian Carcinoma ◽  
Protein Carbonyl ◽  
Protein Carbonyls ◽  
Two Dimensional Gel Electrophoresis ◽  
Carbonyl Protein ◽  
Wide Range ◽  
Modified Proteins ◽  
Oxidatively Modified ◽  
Oxidatively Modified Proteins

Serous subtype of ovarian cancer is considered to originate from fallopian epithelium mucosa that has been exposed to physiological changes resulting from ovulation. Ovulation influences an increased in inflammation of epithelial ovarian cells as results of constant exposure of cells to ROS. The imbalance between ROS and antioxidant capacities, as well as a disruption of redox signaling, causes a wide range of damage to DNA, proteins, and lipids. This study applied spectrophotometric, dinitrophenylhydrazone (DNPH) assay, two-dimensional gel electrophoresis, and Western blot analyses to assess the levels of oxidatively modified proteins in 100 primary serous epithelial ovarian carcinoma and normal/surrounding tissues. These samples were obtained from 56 Caucasian and 44 African-American patients within the age range of61±10years. Analyses showed that the levels of reactive protein carbonyl groups increased as stages progressed to malignancy. Additionally, the levels of protein carbonyls in serous ovarian carcinoma among African Americans are 40% (P<0.05) higher relative to Caucasian at similar advanced stages. Results suggest that oxidative stress is involved in the modification of carbonyl protein groups, leading to increased aggressiveness of epithelial ovarian tumors and may contribute to the disease's invasiveness among African Americans.

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