scholarly journals Differential Accumulation of Transcripts Encoding Sulfur Assimilation Enzymes upon Sulfur and/or Nitrogen Deprivation inArabidopsis thaliana

1999 ◽  
Vol 63 (4) ◽  
pp. 762-766 ◽  
Author(s):  
Yube YAMAGUCHI ◽  
Tatsuo NAKAMURA ◽  
Emiko HARADA ◽  
Nozomu KOIZUMI ◽  
Hiroshi SANO
Keyword(s):  
Nitrogen Deprivation ◽  
Sulfur Assimilation

scholarly journals The bundle sheath of rice is conditioned to play an active role in water transport as well as sulfur assimilation and jasmonic acid synthesis

2021 ◽  
Author(s):  
Lei Hua ◽  
Sean R. Stevenson ◽  
Ivan Reyna‐Llorens ◽  
Haiyan Xiong ◽  
Stanislav Kopriva ◽  
...  
Keyword(s):  
Jasmonic Acid ◽  
Water Transport ◽  
Active Role ◽  
Acid Synthesis ◽  
Bundle Sheath ◽  
Sulfur Assimilation

Nitrogen deprivation induces cross-tolerance of Poa annua callus to salt stress

2016 ◽  
Vol 60 (3) ◽  
pp. 543-554 ◽  
Author(s):  
C. Z. Zhao ◽  
P. Li ◽  
X. M. Wang ◽  
P. Li ◽  
X. Y. Wang ◽  
...  
Keyword(s):  
Salt Stress ◽  
Poa Annua ◽  
Nitrogen Deprivation ◽  
Cross Tolerance

Ammonium and nitrate uptake by soybean during recovery from nitrogen deprivation

1994 ◽  
Vol 45 (1) ◽  
pp. 23-33 ◽  
Author(s):  
James W. Rideout ◽  
Sylvain Chaillou ◽  
C. David Raper ◽  
Jean-François Morot-Gaudry
Keyword(s):  
Nitrate Uptake ◽  
Nitrogen Deprivation

scholarly journals All1371 is a polyphosphate-dependent glucokinase in Anabaena sp. PCC 7120

Microbiology ◽  
2014 ◽  
Vol 160 (12) ◽  
pp. 2807-2819 ◽  
Author(s):  
Friederike Klemke ◽  
Gabriele Beyer ◽  
Linda Sawade ◽  
Ali Saitov ◽  
Thomas Korte ◽  
...  
Keyword(s):  
Divalent Cations ◽  
Enzymic Activity ◽  
Phosphoryl Group ◽  
Nitrogen Deprivation ◽  
Ping Pong ◽  
Nucleoside Triphosphates ◽  
Anabaena Sp ◽  
Pcc 7120 ◽  
Promoter Studies

The polyphosphate glucokinases can phosphorylate glucose to glucose 6-phosphate using polyphosphate as the substrate. ORF all1371 encodes a putative polyphosphate glucokinase in the filamentous heterocyst-forming cyanobacterium Anabaena sp. PCC 7120. Here, ORF all1371 was heterologously expressed in Escherichia coli, and its purified product was characterized. Enzyme activity assays revealed that All1371 is an active polyphosphate glucokinase that can phosphorylate both glucose and mannose in the presence of divalent cations in vitro. Unlike many other polyphosphate glucokinases, for which nucleoside triphosphates (e.g. ATP or GTP) act as phosphoryl group donors, All1371 required polyphosphate to confer its enzymic activity. The enzymic reaction catalysed by All1371 followed classical Michaelis–Menten kinetics, with k cat = 48.2 s−1 at pH 7.5 and 28 °C and K M = 1.76 µM and 0.118 mM for polyphosphate and glucose, respectively. Its reaction mechanism was identified as a particular multi-substrate mechanism called the ‘bi-bi ping-pong mechanism’. Bioinformatic analyses revealed numerous polyphosphate-dependent glucokinases in heterocyst-forming cyanobacteria. Viability of an Anabaena sp. PCC 7120 mutant strain lacking all1371 was impaired under nitrogen-fixing conditions. GFP promoter studies indicate expression of all1371 under combined nitrogen deprivation. All1371 might play a substantial role in Anabaena sp. PCC 7120 under these conditions.

The Mycobacterium tuberculosis cysD and cysNC genes form a stress-induced operon that encodes a tri-functional sulfate-activating complex

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1681-1686 ◽  
Author(s):  
Rachel Pinto ◽  
Quing Xui Tang ◽  
Warwick J. Britton ◽  
Thomas S. Leyh ◽  
James A. Triccas
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sulfur Metabolism ◽  
Enzyme Complex ◽  
Sulfur Assimilation ◽  
Atp Sulfurylase ◽  
Catalytic Activities ◽  
Disease Relevance ◽  
Anti Oxidant ◽  
Sulfate Activation ◽  
Reductive Assimilation

Sulfur metabolism has been implicated in the virulence, antibiotic resistance and anti-oxidant defence of Mycobacterium tuberculosis. Despite its human disease relevance, sulfur metabolism in mycobacteria has not yet been fully characterized. ATP sulfurylase catalyses the synthesis of activated sulfate (adenosine 5′-phosphosulfate, APS), the first step in the reductive assimilation of sulfate. Expression of the M. tuberculosis cysD gene, predicted to encode the adenylyl-transferase subunit of ATP sulfurylase, is upregulated by the bacilli inside its preferred host, the macrophage. This study demonstrates that cysD and cysNC orthologues exist in M. tuberculosis and constitute an operon whose expression is induced by sulfur limitation and repressed by the presence of cysteine, a major end-product of sulfur assimilation. The cysDNC genes are also induced upon exposure to oxidative stress, suggesting regulation of sulfur assimilation by M. tuberculosis in response to toxic oxidants. To ensure that the cysDNC operon encoded the activities predicted by its primary sequence, and to begin to characterize the products of the operon, they were expressed in Escherichia coli, purified to homogeneity, and tested for their catalytic activities. The CysD and CysNC proteins were shown to form a multifunctional enzyme complex that exhibits the three linked catalytic activities that constitute the sulfate activation pathway.

scholarly journals The effect of temperature and nitrogen deprivation on cell morphology and physiology of Symbiodinium

Oceanologia ◽  
2016 ◽  
Vol 58 (4) ◽  
pp. 272-278 ◽  
Author(s):  
Buntora Pasaribu ◽  
Yu-Si Li ◽  
Ping-Chung Kuo ◽  
I-Ping Lin ◽  
Kwee Siong Tew ◽  
...  
Keyword(s):  
Cell Morphology ◽  
Effect Of Temperature ◽  
Nitrogen Deprivation

How Does Evolution in Phosphorus-Impoverished Landscapes Impact Plant Nitrogen and Sulfur Assimilation?

2019 ◽  
Vol 24 (1) ◽  
pp. 69-82 ◽  
Author(s):  
M. Asaduzzaman Prodhan ◽  
Patrick M. Finnegan ◽  
Hans Lambers
Keyword(s):  
Sulfur Assimilation ◽  
Plant Nitrogen

scholarly journals Modulation of intestinal sulfur assimilation metabolism regulates iron homeostasis

2018 ◽  
Vol 115 (12) ◽  
pp. 3000-3005 ◽  
Author(s):  
Benjamin H. Hudson ◽  
Andrew T. Hale ◽  
Ryan P. Irving ◽  
Shenglan Li ◽  
John D. York
Keyword(s):  
Iron Deficiency ◽  
Iron Deficiency Anemia ◽  
Iron Reduction ◽  
Genetic Basis ◽  
Iron Homeostasis ◽  
Deficiency Anemia ◽  
Sulfur Assimilation ◽  
Nucleotide Hydrolysis ◽  
Evolutionarily Conserved ◽  
Organismal Development

Sulfur assimilation is an evolutionarily conserved pathway that plays an essential role in cellular and metabolic processes, including sulfation, amino acid biosynthesis, and organismal development. We report that loss of a key enzymatic component of the pathway, bisphosphate 3′-nucleotidase (Bpnt1), in mice, both whole animal and intestine-specific, leads to iron-deficiency anemia. Analysis of mutant enterocytes demonstrates that modulation of their substrate 3′-phosphoadenosine 5′-phosphate (PAP) influences levels of key iron homeostasis factors involved in dietary iron reduction, import and transport, that in part mimic those reported for the loss of hypoxic-induced transcription factor, HIF-2α. Our studies define a genetic basis for iron-deficiency anemia, a molecular approach for rescuing loss of nucleotidase function, and an unanticipated link between nucleotide hydrolysis in the sulfur assimilation pathway and iron homeostasis.

scholarly journals Transcriptomic survey reveals multiple adaptation mechanisms in response to nitrogen deprivation in marine Porphyridium cruentum

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259833
Author(s):  
Li Wei ◽  
Wuxin You ◽  
Zhengru Xu ◽  
Wenfei Zhang
Keyword(s):  
Lipid Metabolism ◽  
De Novo ◽  
Nitrate Assimilation ◽  
Porphyridium Cruentum ◽  
Metabolic Reprogramming ◽  
Nitrogen Stress ◽  
Nitrogen Deprivation ◽  
Marine Microalga ◽  
Nutrient Environment ◽  
Red Microalga

Single-cell red microalga Porphyridium cruentum is potentially considered to be the bioresource for biofuel and pharmaceutical production. Nitrogen is a kind of nutrient component for photosynthetic P. cruentum. Meanwhile, nitrogen stress could induce to accumulate some substances such as lipid and phycoerythrin and affect its growth and physiology. However, how marine microalga Porphyridium cruentum respond and adapt to nitrogen starvation remains elusive. Here, acclimation of the metabolic reprogramming to changes in the nutrient environment was studied by high-throughput mRNA sequencing in the unicellular red alga P. cruentum. Firstly, to reveal transcriptional regulation, de novo transcriptome was assembled and 8,244 unigenes were annotated based on different database. Secondly, under nitrogen deprivation, 2100 unigenes displayed differential expression (1134 upregulation and 966 downregulation, respectively) and some pathways including carbon/nitrogen metabolism, photosynthesis, and lipid metabolism would be reprogrammed in P. cruentum. The result demonstrated that nitrate assimilation (with related unigenes of 8–493 fold upregulation) would be strengthen and photosynthesis (with related unigenes of 6–35 fold downregulation) be impaired under nitrogen deprivation. Importantly, compared to other green algae, red microalga P. cruentum presented a different expression pattern of lipid metabolism in response to nitrogen stress. These observations will also provide novel insight for understanding adaption mechanisms and potential targets for metabolic engineering and synthetic biology in P. cruentum.

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