DNA Cleavage Activities of (-)-Epigallocatechin, (-)-Epicatechin, (+)-Catechin, and (-)-Epigallocatechin Gallate with Various Kind of Metal Ions

1999 ◽  
Vol 63 (9) ◽  
pp. 1654-1656 ◽  
Author(s):  
Fumiko HAYAKAWA ◽  
Takahide KIMURA ◽  
Nobuo HOSHINO ◽  
Takashi ANDO
Keyword(s):  
Metal Ions ◽  
Dna Cleavage ◽  
Epigallocatechin Gallate

An intelligent NIR-responsive chelate copper-based anticancer nanoplatform for synergistic tumor targeted chemo-phototherapy

Nanoscale ◽  
2017 ◽  
Vol 9 (40) ◽  
pp. 15685-15695 ◽  
Author(s):  
Qianhua Feng ◽  
Wanxia Zhang ◽  
Yuzhen Li ◽  
Xuemei Yang ◽  
Yongwei Hao ◽  
...  
Keyword(s):  
Metal Ions ◽  
Anticancer Drug ◽  
Dna Cleavage ◽  
Copper Ion ◽  
Ion Release ◽  
Temperature Responsive

The chelate copper-based anticancer drug bleomycin (BLM) is usually believed to bind metal ions especially Cu(ii) to generate the “activated BLM” for DNA cleavage. This study offered a platform for simultaneous NIR-mediated temperature-responsive BLM and copper ion release, which further initiated the generation of the “activated BLM”.

Endonuclease Active Site Plasticity Allows DNA Cleavage with Diverse Alkaline Earth and Transition Metal Ions

2011 ◽  
Vol 6 (9) ◽  
pp. 934-942 ◽  
Author(s):  
Kommireddy Vasu ◽  
Matheshwaran Saravanan ◽  
Valakunja Nagaraja
Keyword(s):  
Transition Metal ◽  
Metal Ions ◽  
Active Site ◽  
Dna Cleavage ◽  
Alkaline Earth ◽  
Transition Metal Ions

scholarly journals Use of Divalent Metal Ions in the DNA Cleavage Reaction of Human Type II Topoisomerases†

Biochemistry ◽  
2009 ◽  
Vol 48 (9) ◽  
pp. 1862-1869 ◽  
Author(s):  
Joseph E. Deweese ◽  
Amber M. Burch ◽  
Alex B. Burgin ◽  
Neil Osheroff
Keyword(s):  
Metal Ions ◽  
Dna Cleavage ◽  
Divalent Metal ◽  
Type Ii ◽  
Divalent Metal Ions ◽  
Cleavage Reaction ◽  
Human Type

DNA cleavage by the EcoRV restriction endonuclease: roles of divalent metal ions in specificity and catalysis

1999 ◽  
Vol 288 (1) ◽  
pp. 87-103 ◽  
Author(s):  
Geoffrey S. Baldwin ◽  
Richard B. Sessions ◽  
Symon G. Erskine ◽  
Stephen E. Halford
Keyword(s):  
Metal Ions ◽  
Restriction Endonuclease ◽  
Dna Cleavage ◽  
Divalent Metal ◽  
Divalent Metal Ions

scholarly journals Site-specific DNA cleavage by mammalian DNA topoisomerase II induced by novel flavone and catechin derivatives

1992 ◽  
Vol 282 (3) ◽  
pp. 883-889 ◽  
Author(s):  
C A Austin ◽  
S Patel ◽  
K Ono ◽  
H Nakane ◽  
L M Fisher
Keyword(s):  
Dna Cleavage ◽  
Topoisomerase Ii ◽  
Complex Analysis ◽  
Antiviral Agents ◽  
Epigallocatechin Gallate ◽  
Tea Plant ◽  
Epicatechin Gallate ◽  
Pyrone Ring ◽  
Cleavable Complex

Four naturally occurring flavones (baicalein, quercetin, quercetagetin and myricetin) and two novel catechins [(-)-epicatechin gallate and (-)-epigallocatechin gallate, from the tea plant Camellia sinensis], which are known inhibitors of reverse transcriptase, were shown to induce mammalian topoisomerase II-dependent DNA-cleavage in vitro. The flavones differed from the catechins in causing unwinding of duplex DNA, but both classes of compound induced enzymic DNA breakage at the same sites on DNA. Moreover, the cleavage specificity was the same as that for the known intercalator 4′-(acridin-9-ylamino)methanesulphon-m-anisidide, suggesting that these agents trap the same cleavable complex. Analysis of some 30 flavonoid compounds allowed elucidation of the structure-function relationships for topoisomerase II-mediated DNA cleavage. For flavonoid inhibitors an unsaturated double bond between positions 2 and 3 of the pyrone ring and hydroxy groups at the 5, 7, 3′ and 4′ positions favoured efficient cleavage. Hydroxy substitutions could be tolerated at the 3, 6 and 5′ positions. Indeed, the absence of substituents at the 3′, 4′ and 5′ positions could be compensated by a hydroxy group at position 6 (baicalein). Similar requirements have been reported for flavonoid inhibitors of protein kinase C that act competitively with ATP, suggesting interaction with a conserved protein feature. Formation of the cleavable complex is a cytotoxic lesion that may contribute to the growth-inhibitory properties of flavones observed for three human tumour cell lines. These results are discussed in regard to the selectivity of antiviral agents.

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