Involvement of Cytochromeain Iron Oxidation of a Moderately Thermophilic Iron-Oxidizing Bacterium, Strain TI-1

1999 ◽  
Vol 63 (9) ◽  
pp. 1541-1547 ◽  
Author(s):  
Masaki TAKAI ◽  
Kazuo KAMIMURA ◽  
Tsuyoshi SUGIO
Keyword(s):  
Iron Oxidation ◽  
Moderately Thermophilic ◽  
Bacterium Strain

Ferrous iron oxidation in moderately thermophilic acidophile Sulfobacillus sibiricus N1T

2010 ◽  
Vol 56 (10) ◽  
pp. 803-808 ◽  
Author(s):  
Tatiana Y. Dinarieva ◽  
Anna E. Zhuravleva ◽  
Oksana A. Pavlenko ◽  
Iraida A. Tsaplina ◽  
Alexander I. Netrusov
Keyword(s):  
Ferrous Iron ◽  
High Performance ◽  
Iron Oxidation ◽  
Early Exponential Phase ◽  
Moderately Thermophilic ◽  
Terminal Oxidases ◽  
Cytochrome Bo ◽  
Ferrous Iron Oxidation ◽  
Transport Chain ◽  
Membrane Bound

The iron-oxidizing system of a moderately thermophilic, extremely acidophilic, gram-positive mixotroph, Sulfobacillus sibiricus N1T, was studied by spectroscopic, high-performance liquid chromatography and inhibitory analyses. Hemes B, A, and O were detected in membranes of S. sibiricus N1T. It is proposed that the electron transport chain from Fe2+ to O2 is terminated by 2 physiological oxidases: aa3-type cytochrome, which dominates in the early-exponential phase of growth, and bo3-type cytochrome, whose role in iron oxidation becomes more prominent upon growth of the culture. Both oxidases were sensitive to cyanide and azide. Cytochrome aa3 was more sensitive to cyanide and azide, with Ki values of 4.1 and 2.5 µmol·L–1, respectively, compared with Ki values for cytochrome bo3, which were 9.5 µmol·L–1 for cyanide and 7.0 µmol·L–1 for azide. This is the first evidence for the participation of a bo3-type oxidase in ferrous iron oxidation. The respiratory chain of the mixotroph contains, in addition to the 2 terminal oxidases, a membrane-bound cytochrome b573.

Kinetics of Ferrous Iron Oxidation by Moderately Thermophilic Microorganisms

2007 ◽  
Vol 20-21 ◽  
pp. 517-520
Author(s):  
Pablo S. Pina ◽  
V.A. Leão ◽  
C.A. Silva ◽  
S.N. Medrício ◽  
J. Frenay
Keyword(s):  
Ferrous Iron ◽  
Iron Oxidation ◽  
Thermophilic Microorganisms ◽  
Moderately Thermophilic ◽  
Ferrous Iron Oxidation ◽  
Kinetics Of

scholarly journals Clostridium straminisolvens sp. nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community

2004 ◽  
Vol 54 (6) ◽  
pp. 2043-2047 ◽  
Author(s):  
Souichiro Kato ◽  
Shin Haruta ◽  
Zong Jun Cui ◽  
Masaharu Ishii ◽  
Akira Yokota ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gene Sequence ◽  
Cellulose Degradation ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Cellulolytic Bacterium ◽  
Moderately Thermophilic ◽  
Initial Ph ◽  
Bacterium Strain ◽  
Curved Rod

A novel anaerobic, thermophilic and cellulolytic bacterium (strain CSK1T) was isolated from a cellulose-degrading bacterial community. On the basis of 16S rRNA gene sequence similarity, strain CSK1T was mapped to cluster III of the genus Clostridium. Strain CSK1T is closely related to Clostridium thermocellum (96·2 %) and Clostridium aldrichii (95·1 %). Strain CSK1T is a non-motile, spore-forming, straight or slightly curved rod. The optimum temperature and initial pH for its growth and cellulose degradation are 50–55 °C and pH 7·5. Strain CSK1T grew under a gas phase containing up to 4 % O2. Phylogenetic and phenotypic analyses support the differentiation of strain CSK1T from its closest relatives. Strain CSK1T therefore represents a novel species, for which the name Clostridium straminisolvens sp. nov. is proposed, with CSK1T (=DSM 16021T=IAM 15070T) as the type strain.

scholarly journals Petrobacter succinatimandens gen. nov., sp. nov., a moderately thermophilic, nitrate-reducing bacterium isolated from an Australian oil well

2004 ◽  
Vol 54 (3) ◽  
pp. 645-649 ◽  
Author(s):  
Monica Bonilla Salinas ◽  
Marie-Laure Fardeau ◽  
Jean-Luc Cayol ◽  
Laurence Casalot ◽  
Bharat K. C. Patel ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Growth Conditions ◽  
Thermophilic Bacterium ◽  
Oil Reservoir ◽  
Oil Well ◽  
Terminal Electron Acceptor ◽  
Gram Negative ◽  
Moderately Thermophilic ◽  
Bacterium Strain

A novel Gram-negative, aerobic and moderately thermophilic bacterium, strain 4BONT, was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 °C, pH 6·9 and 0·5 % NaCl. Strain 4BONT was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58·6 mol%. The closest phylogenetic relative of strain 4BONT was Hydrogenophilus thermoluteolus (similarity of 91·8 %), of the β-Proteobacteria. As strain 4BONT is physiologically and phylogenetically different from H. thermoluteolus, it is proposed that it be assigned to a novel species of a novel genus, Petrobacter succinatimandens gen. nov., sp. nov. The type strain is 4BONT (=DSM 15512T=CIP 107790T).

Resistance of Moderately Thermophilic Acidophilic Microorganisms to Ferric Iron Ions

2017 ◽  
Vol 262 ◽  
pp. 471-475
Author(s):  
Aleksander Bulaev
Keyword(s):  
Ferrous Iron ◽  
Ferric Iron ◽  
Iron Oxidation ◽  
Iron Concentration ◽  
Iron Ions ◽  
Moderately Thermophilic ◽  
Ferrous Iron Oxidation ◽  
Low Concentrations ◽  
The Media ◽  
High Concentrations

Resistance of microorganisms predominating in biohydrometallurgical processes including bacteria of the genus Sulfobaсillus and archaea of the genus Acidiplasma to ferric iron ions was studied. Capabilities of the strains for growth and ferrous iron oxidation in the media containing high concentrations of ferric iron ions (of 250 to 1000 mM) were evaluated. Ferric iron ions significantly inhibited oxidative activity and growth of the studied microorganisms. It was revealed that bacteria of the genus Sulfobacillus were not able to oxidize ferrous iron actively when ferric iron concentration exceeded 500 mM, whereas archaea of the genus Acidiplasma completely oxidized ferrous iron in the medium containing 1000 mM of Fe3+. Growth of the microorganisms was inhibited by relatively low concentrations of ferric iron. Microorganisms did not grow in the medium containing more than 750 mM of Fe3+ and cells of all studied strains lysed in presence of high concentrations of ferric iron. It was shown, that archaea of the genus Acidiplasma of the family Ferroplasmaceae were more resistant to high concentrations of ferric iron than bacteria of the genus Sulfobacillus. The results obtained are important for understanding of the regularities of the formation of microbial communities performing technological processes.

Anoxybacillus vitaminiphilus sp. nov., a strictly aerobic and moderately thermophilic bacterium isolated from a hot spring

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4064-4071 ◽  
Author(s):  
Xin-Qi Zhang ◽  
Zhen-Li Zhang ◽  
Nan Wu ◽  
Xu-Fen Zhu ◽  
Min Wu
Keyword(s):  
Hot Spring ◽  
Novel Species ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Bacterium Strain

A strictly aerobic, Gram-stain-positive, motile and spore-forming bacterium, strain 3nP4T, was isolated from the Puge hot spring located in the south-western geothermal area of China. Strain 3nP4T grew at 38–66 °C (optimum 57–60 °C), at pH 6.0–9.3 (optimum 7.0–7.5) and with 0–4 % (w/v) NaCl (optimum 0–0.5 %). Phylogenetic analysis of 16S rRNA gene sequences, as well as DNA–DNA relatedness values, indicated that the isolate represents a novel species of the genus Anoxybacillus , related most closely to Anoxybacillus voinovskiensis DSM 12111T. Strain 3nP4T had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid as major polar lipids and iso-C15 : 0 and iso-C17 : 0 as major fatty acids, which are both typical chemotaxonomic characteristics of the genus Anoxybacillus . The mean DNA G+C content of strain 3nP4T was 39.2±0.95 mol% (HPLC). A distinctive characteristic of the novel isolate was its extreme reliance on vitamin mixture or yeast extract for growth. Based on data from this taxonomic study using a polyphasic approach, strain 3nP4T is considered to represent a novel species of the genus Anoxybacillus , for which the name Anoxybacillus vitaminiphilus sp. nov. is proposed. The type strain is 3nP4T ( = CGMCC 1.8979T = JCM 16594T).

scholarly journals Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales

2006 ◽  
Vol 56 (2) ◽  
pp. 369-372 ◽  
Author(s):  
A. I. Slobodkin ◽  
T. P. Tourova ◽  
N. A. Kostrikina ◽  
A. M. Lysenko ◽  
K. E. German ◽  
...  
Keyword(s):  
Hot Spring ◽  
Phylogenetic Analyses ◽  
Casein Hydrolysate ◽  
Rrna Gene ◽  
Donor Strain ◽  
Moderately Thermophilic ◽  
Physiological Properties ◽  
Bacterium Strain ◽  
Casamino Acids ◽  
Ph Range

A moderately thermophilic, anaerobic bacterium (strain SB91T) was isolated from a freshwater hot spring at Barguzin Valley, Buryatiya, Russia. Cells of strain SB91T were straight to slightly curved rods, 0·5–0·6 μm in diameter and 3·0–7·0 μm in length. Formation of endospores was not observed. The temperature range for growth was 26–62 °C, with an optimum at 50 °C. The pH range for growth was 5·5–9·5, with an optimum at pH 7·5–8·0. The substrates utilized by strain SB91T in the presence of 9,10-anthraquinone 2,6-disulfonate included peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, alanine plus glycine, alanine plus proline, l-valine and n-propanol. Carbohydrates were not utilized. Strain SB91T reduced amorphous Fe(III) oxide, Fe(III) citrate, Fe(III) EDTA or Fe(III) nitrilotriacetate with peptone, l-valine or n-propanol as an electron donor. Strain SB91T reduced 9,10-anthraquinone 2,6-disulfonate, thiosulfate, elemental sulfur, fumarate and selenite. Strain SB91T survived after exposure to gamma-radiation at a dose of 5·4 kGy. The G+C content of the DNA of strain SB91T was 33 mol%. Analysis of the 16S rRNA gene sequence revealed that the isolated organism belonged to cluster XII of the clostridia. On the basis of its physiological properties and the results of phylogenetic analyses, it is proposed that strain SB91T represents the sole species of a novel genus, Tepidimicrobium; the name Tepidimicrobium ferriphilum gen. nov., sp. nov. is proposed, with strain SB91T (=DSM 16624T=VKM B-2348T) as the type strain.

A new iron oxidase from a moderately thermophilic iron oxidizing bacterium strain TI-1

2001 ◽  
Vol 268 (6) ◽  
pp. 1653-1658
Author(s):  
Masaki Takai ◽  
Kazuo Kamimura ◽  
Tsuyoshi Sugio
Keyword(s):  
Moderately Thermophilic ◽  
Bacterium Strain

scholarly journals Desulfotomaculum carboxydivorans sp. nov., a novel sulfate-reducing bacterium capable of growth at 100 % CO

2005 ◽  
Vol 55 (5) ◽  
pp. 2159-2165 ◽  
Author(s):  
Sofiya N. Parshina ◽  
Jan Sipma ◽  
Yutaka Nakashimada ◽  
Anne Meint Henstra ◽  
Hauke Smidt ◽  
...  
Keyword(s):  
Gas Phase ◽  
Paper Mill ◽  
Rrna Gene ◽  
Moderately Thermophilic ◽  
Sulfate Reducing ◽  
Nacl Concentration ◽  
Bacterium Strain ◽  
Ph Range ◽  
Desulfotomaculum Nigrificans ◽  
Paper Mill Wastewater

A moderately thermophilic, anaerobic, chemolithoheterotrophic, sulfate-reducing bacterium, strain CO-1-SRBT, was isolated from sludge from an anaerobic bioreactor treating paper mill wastewater. Cells were Gram-positive, motile, spore-forming rods. The temperature range for growth was 30–68 °C, with an optimum at 55 °C. The NaCl concentration range for growth was 0–17 g l−1; there was no change in growth rate until the NaCl concentration reached 8 g l−1. The pH range for growth was 6·0–8·0, with an optimum of 6·8–7·2. The bacterium could grow with 100 % CO in the gas phase. With sulfate, CO was converted to H2 and CO2 and part of the H2 was used for sulfate reduction; without sulfate, CO was completely converted to H2 and CO2. With sulfate, strain CO-1-SRBT utilized H2/CO2, pyruvate, glucose, fructose, maltose, lactate, serine, alanine, ethanol and glycerol. The strain fermented pyruvate, lactate, glucose and fructose. Yeast extract was necessary for growth. Sulfate, thiosulfate and sulfite were used as electron acceptors, whereas elemental sulfur and nitrate were not. A phylogenetic analysis of 16S rRNA gene sequences placed strain CO-1-SRBT in the genus Desulfotomaculum, closely resembling Desulfotomaculum nigrificans DSM 574T and Desulfotomaculum sp. RHT-3 (99 and 100 % similarity, respectively). However, the latter strains were completely inhibited above 20 and 50 % CO in the gas phase, respectively, and were unable to ferment CO, lactate or glucose in the absence of sulfate. DNA–DNA hybridization of strain CO-1-SRBT with D. nigrificans and Desulfotomaculum sp. RHT-3 showed 53 and 60 % relatedness, respectively. On the basis of phylogenetic and physiological features, it is suggested that strain CO-1-SRBT represents a novel species within the genus Desulfotomaculum, for which the name Desulfotomaculum carboxydivorans is proposed. This is the first description of a sulfate-reducing micro-organism that is capable of growth under an atmosphere of pure CO with and without sulfate. The type strain is CO-1-SRBT (=DSM 14880T=VKM B-2319T).

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