A Cysteine Protease from Young Stems of Asparagus: Isolation, Properties, and Substrate Specificity

1998 ◽  
Vol 62 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Hiroo YONEZAWA ◽  
Makoto KANEDA ◽  
Tetsuya UCHIKOBA
Keyword(s):  
Substrate Specificity ◽  
Cysteine Protease

scholarly journals Substrate Specificity of the Streptococcal Cysteine Protease

2001 ◽  
Vol 276 (48) ◽  
pp. 44551-44556 ◽  
Author(s):  
Motoyoshi Nomizu ◽  
Grzegorz Pietrzynski ◽  
Tamaki Kato ◽  
Paule Lachance ◽  
Robert Menard ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cysteine Protease

scholarly journals Identification of a Novel Maturation Mechanism and Restricted Substrate Specificity for the SspB Cysteine Protease ofStaphylococcus aureus

2002 ◽  
Vol 277 (44) ◽  
pp. 41770-41777 ◽  
Author(s):  
Isabella Massimi ◽  
Ellen Park ◽  
Kelly Rice ◽  
Werner Müller-Esterl ◽  
Daniel Sauder ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cysteine Protease ◽  
Ofstaphylococcus Aureus

scholarly journals Computational Study on Substrate Specificity of a Novel Cysteine Protease 1 Precursor from Zea mays

2014 ◽  
Vol 15 (6) ◽  
pp. 10459-10478 ◽  
Author(s):  
Huimin Liu ◽  
Liangcheng Chen ◽  
Quan Li ◽  
Mingzhu Zheng ◽  
Jingsheng Liu
Keyword(s):  
Zea Mays ◽  
Substrate Specificity ◽  
Cysteine Protease ◽  
Computational Study

scholarly journals Cohesin cleavage by separase is enhanced by a substrate motif distinct from the cleavage site

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Laura E. Rosen ◽  
Joseph E. Klebba ◽  
Jonathan B. Asfaha ◽  
Chloe M. Ghent ◽  
Melody G. Campbell ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Chromosome Segregation ◽  
Cysteine Protease ◽  
Active Site ◽  
Cleavage Site ◽  
Specific Substrate ◽  
Substrate Docking ◽  
Substrate Cleavage ◽  
Docking Interaction ◽  
Substrate Motif

AbstractChromosome segregation begins when the cysteine protease, separase, cleaves the Scc1 subunit of cohesin at the metaphase-to-anaphase transition. Separase is inhibited prior to metaphase by the tightly bound securin protein, which contains a pseudosubstrate motif that blocks the separase active site. To investigate separase substrate specificity and regulation, here we develop a system for producing recombinant, securin-free human separase. Using this enzyme, we identify an LPE motif on the Scc1 substrate that is distinct from the cleavage site and is required for rapid and specific substrate cleavage. Securin also contains a conserved LPE motif, and we provide evidence that this sequence blocks separase engagement of the Scc1 LPE motif. Our results suggest that rapid cohesin cleavage by separase requires a substrate docking interaction outside the active site. This interaction is blocked by securin, providing a second mechanism by which securin inhibits cohesin cleavage.

scholarly journals Structural basis for catalysis and substrate specificity of a 3C-like cysteine protease from a mosquito mesonivirus

Virology ◽  
2019 ◽  
Vol 533 ◽  
pp. 21-33 ◽  
Author(s):  
Manuel Kanitz ◽  
Sandra Blanck ◽  
Andreas Heine ◽  
Anastasia A. Gulyaeva ◽  
Alexander E. Gorbalenya ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cysteine Protease ◽  
Structural Basis

The Substrate Specificity of a Recombinant Cysteine Protease fromLeishmania mexicana: Application of a Combinatorial Peptide Library Approach

ChemBioChem ◽  
2000 ◽  
Vol 1 (2) ◽  
pp. 115-122 ◽  
Author(s):  
Phaedria M. St. Hilaire ◽  
Lira C. Alves ◽  
Sanya J. Sanderson ◽  
Jeremy C. Mottram ◽  
Maria A. Juliano ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cysteine Protease ◽  
Peptide Library ◽  
Combinatorial Peptide Library ◽  
Peptide Library Approach

scholarly journals The substrate specificity of cruzipain 2, a cysteine protease isoform fromTrypanosoma cruzi

2006 ◽  
Vol 259 (2) ◽  
pp. 215-220 ◽  
Author(s):  
Flavia C. G. dos Reis ◽  
Wagner A. S. Júdice ◽  
Maria A. Juliano ◽  
Luiz Juliano ◽  
Julio Scharfstein ◽  
...  
Keyword(s):  
Substrate Specificity ◽  
Cysteine Protease

Fluorescence-Quenched Solid Phase Combinatorial Libraries in the Characterization of Cysteine Protease Substrate Specificity†

1999 ◽  
Vol 1 (6) ◽  
pp. 509-523 ◽  
Author(s):  
Phaedria M. St. Hilaire ◽  
Marianne Willert ◽  
Maria Aparecida Juliano ◽  
Luiz Juliano ◽  
Morten Meldal
Keyword(s):  
Substrate Specificity ◽  
Cysteine Protease ◽  
Solid Phase ◽  
Combinatorial Libraries ◽  
Protease Substrate
Sign in / Sign up

Export Citation Format

Share Document