Expression of Biologically Active Human Calpastatin in Baculovirus-infected Insect Cells and inEscherichia coli

1998 ◽  
Vol 62 (1) ◽  
pp. 136-141 ◽  
Author(s):  
Kiyotaka HITOMI ◽  
Akiko YOKOYAMA ◽  
Masatoshi MAKI
Gene ◽  
1993 ◽  
Vol 128 (2) ◽  
pp. 307-308 ◽  
Author(s):  
Bernard Y. Amegadzie ◽  
Deborah Jiampetti ◽  
Robert J. Craig ◽  
Edward Appelbaum ◽  
Allan R. Shatzman ◽  
...  

1997 ◽  
Vol 9 (1) ◽  
pp. 100-108 ◽  
Author(s):  
Kari J. Airenne ◽  
Christian Oker-Blom ◽  
Varpu S. Marjomäki ◽  
Edward A. Bayer ◽  
Meir Wilchek ◽  
...  

2009 ◽  
Vol 68 (1) ◽  
pp. 90-98 ◽  
Author(s):  
B. Shay ◽  
Y. Gruenbaum-Cohen ◽  
A.S. Tucker ◽  
A.L. Taylor ◽  
E. Rosenfeld ◽  
...  

Gene ◽  
1991 ◽  
Vol 101 (2) ◽  
pp. 255-260 ◽  
Author(s):  
Luca Benatti ◽  
Emanuela Scacheri ◽  
David H.L. Bishop ◽  
Paolo Sarmientos

2009 ◽  
Vol 41 (3) ◽  
pp. 289-297 ◽  
Author(s):  
Marianna Madeo ◽  
Chiara Carrisi ◽  
Domenico Iacopetta ◽  
Loredana Capobianco ◽  
Anna Rita Cappello ◽  
...  

1992 ◽  
Vol 286 (3) ◽  
pp. 677-680 ◽  
Author(s):  
J D Robishaw ◽  
V K Kalman ◽  
K L Proulx

As a result of the inability to resolve the heterogeneous mixture of G protein beta gamma subunits present in tissues, it has not been possible to compare different beta gamma subunits of the G proteins in terms of their proposed roles in receptor-effector coupling. This study was undertaken to establish the utility of the baculovirus expression system in producing homogeneous beta gamma subunits of defined composition for the comparative analysis of these subunits in reconstitution systems. In this study we report the expression, and appropriate post-translational processing, of recombinant beta 2, gamma 2 and gamma 3 subunits. In addition, we show that the recombinant beta gamma subunits can be readily purified, and can functionally interact with the alpha subunits of the G proteins.


1994 ◽  
Vol 14 (2) ◽  
pp. 99-117 ◽  
Author(s):  
J. A. Javitch ◽  
J. Kaback ◽  
X. Li ◽  
A. Karlin
Keyword(s):  

1982 ◽  
Vol 10 (16) ◽  
pp. 4883-4890 ◽  
Author(s):  
Maria L. Gennaro ◽  
Peter J. Greenaway ◽  
David A. Broadbent

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