Chemical Modification of the Hemolytic Lectin CEL-III by Succinic Anhydride: Involvement of Amino Groups in the Oligomerization Process

1998 ◽  
Vol 62 (6) ◽  
pp. 1185-1189 ◽  
Author(s):  
Tomomitsu HATAKEYAMA ◽  
Yumiko MATSUYAMA ◽  
Takako FUNADA ◽  
Sachiko FUKUYAMA ◽  
Hiromiki KUWAHARA ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Succinic Anhydride ◽  
Amino Groups

scholarly journals Chemical modification studies on a lectin from winged-bean [Psophocarpus tetragonolobus (L.) DC] tubers

1988 ◽  
Vol 254 (2) ◽  
pp. 351-357 ◽  
Author(s):  
M S Shet ◽  
M Madaiah
Keyword(s):  
Acetic Anhydride ◽  
Chemical Modification ◽  
Succinic Anhydride ◽  
Structural Changes ◽  
Dicarboxylic Acids ◽  
Winged Bean ◽  
Amino Groups ◽  
Reductive Methylation ◽  
Tryptophan Residues ◽  
Haemagglutinating Activity

The effect of chemical modification on a D(+)-galactose-specific lectin isolated from winged-bean tubers was investigated to identify the type of amino acid involved in its haemagglutinating activity. Various anhydrides of dicarboxylic acids, such as acetic anhydride, succinic anhydride, maleic anhydride and citraconic anhydride, modified 57-68% of the amino groups of the winged-bean tuber lectin. Treatment with N-acetylimidazole modified only 45% of the total amino groups. Reductive methylation of free amino groups modified 57% of the amino groups. Modification of the amino groups of the lectin by acetic anhydride and succinic anhydride did not lead to any significant change in the haemagglutinating activity (greater than or equal to 75% active). However, citraconylation and maleylation of the lectin led to a significant decrease in the haemagglutinating activity (less than or equal to 20% active). Acetylation and succinylation (3-carboxypropionylation) of the lectin led to a decrease in the pI value of the native lectin from approx. 9.5 to approx. 4.5. Treatment of the lectin with N-bromosuccinimide led to the modification of two and four tryptophan residues per molecule in the absence and in the presence of 8 M-urea respectively. The immunological identity of all the modified lectin preparations showed no gross structural changes except the lectin modified with N-bromosuccinimide in the presence of urea at pH 4.0.

scholarly journals Chemical modification of the adeno-associated virus capsid to improve gene delivery

2020 ◽  
Vol 11 (4) ◽  
pp. 1122-1131 ◽  
Author(s):  
Mathieu Mével ◽  
Mohammed Bouzelha ◽  
Aurélien Leray ◽  
Simon Pacouret ◽  
Mickael Guilbaud ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Chemical Modification ◽  
Neutralizing Antibodies ◽  
Cell Tropism ◽  
Amino Groups ◽  
Chemical Modifications ◽  
Virus Capsid ◽  
Adeno Associated Virus

Bioconjugated AAV vectors, achieved by coupling of ligands on amino groups of the capsid, are of great interest for gene delivery. Chemical modifications can be used to enhance cell tropism and to decrease interactions with neutralizing antibodies.

Application of Hyperbranched Polymer with Terminal Amino in Dyeing Process of Microfiber Synthetic Leather

2011 ◽  
Vol 393-395 ◽  
pp. 1114-1118
Author(s):  
Long Fang Ren ◽  
Guo Hui Zhao ◽  
Tao Tao Qiang ◽  
Jing Xian Wang ◽  
Xue Chuan Wang
Keyword(s):  
Uptake Rate ◽  
Hyperbranched Polymer ◽  
Succinic Anhydride ◽  
Amino Group ◽  
Dye Uptake ◽  
Amino Groups ◽  
Synthetic Leather ◽  
Dyeing Process ◽  
Terminal Amino ◽  
Polymerization Method

Hyperbranched polymer with different contents of terminal amino group synthesized with succinic anhydride and DETA through the molten polymerization method was used in the dying process of microfiber synthetic leather substrate as color fixing agent. The effect on dye-uptake, surface chromas of microfiber synthetic leather substrate, wet and dry rub fastness was discussed. The result indicated that when the dosage of hyperbranched polymer with 5.85% terminal amino groups was 0.8%, the dye uptake rate was 92.92% and surface chroma was the best, the wet and dry rub fastness of microfiber synthetic leather substrate were almost unchanged.

Sign in / Sign up

Export Citation Format

Share Document