scholarly journals Thiosulfate Reductase from a Moderately Thermophilic Iron-oxidizing Bacterium, Strain TI-1—Purification and Characterization

1997 ◽  
Vol 61 (3) ◽  
pp. 470-474 ◽  
Author(s):  
Tsuyoshi Sugio ◽  
Kimiko Kishimoto ◽  
Keiichi Oda
Keyword(s):  
Purification And Characterization ◽  
Moderately Thermophilic ◽  
Thiosulfate Reductase ◽  
Bacterium Strain

scholarly journals Clostridium straminisolvens sp. nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community

2004 ◽  
Vol 54 (6) ◽  
pp. 2043-2047 ◽  
Author(s):  
Souichiro Kato ◽  
Shin Haruta ◽  
Zong Jun Cui ◽  
Masaharu Ishii ◽  
Akira Yokota ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Gene Sequence ◽  
Cellulose Degradation ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
Cellulolytic Bacterium ◽  
Moderately Thermophilic ◽  
Initial Ph ◽  
Bacterium Strain ◽  
Curved Rod

A novel anaerobic, thermophilic and cellulolytic bacterium (strain CSK1T) was isolated from a cellulose-degrading bacterial community. On the basis of 16S rRNA gene sequence similarity, strain CSK1T was mapped to cluster III of the genus Clostridium. Strain CSK1T is closely related to Clostridium thermocellum (96·2 %) and Clostridium aldrichii (95·1 %). Strain CSK1T is a non-motile, spore-forming, straight or slightly curved rod. The optimum temperature and initial pH for its growth and cellulose degradation are 50–55 °C and pH 7·5. Strain CSK1T grew under a gas phase containing up to 4 % O2. Phylogenetic and phenotypic analyses support the differentiation of strain CSK1T from its closest relatives. Strain CSK1T therefore represents a novel species, for which the name Clostridium straminisolvens sp. nov. is proposed, with CSK1T (=DSM 16021T=IAM 15070T) as the type strain.

scholarly journals Petrobacter succinatimandens gen. nov., sp. nov., a moderately thermophilic, nitrate-reducing bacterium isolated from an Australian oil well

2004 ◽  
Vol 54 (3) ◽  
pp. 645-649 ◽  
Author(s):  
Monica Bonilla Salinas ◽  
Marie-Laure Fardeau ◽  
Jean-Luc Cayol ◽  
Laurence Casalot ◽  
Bharat K. C. Patel ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Growth Conditions ◽  
Thermophilic Bacterium ◽  
Oil Reservoir ◽  
Oil Well ◽  
Terminal Electron Acceptor ◽  
Gram Negative ◽  
Moderately Thermophilic ◽  
Bacterium Strain

A novel Gram-negative, aerobic and moderately thermophilic bacterium, strain 4BONT, was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 °C, pH 6·9 and 0·5 % NaCl. Strain 4BONT was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58·6 mol%. The closest phylogenetic relative of strain 4BONT was Hydrogenophilus thermoluteolus (similarity of 91·8 %), of the β-Proteobacteria. As strain 4BONT is physiologically and phylogenetically different from H. thermoluteolus, it is proposed that it be assigned to a novel species of a novel genus, Petrobacter succinatimandens gen. nov., sp. nov. The type strain is 4BONT (=DSM 15512T=CIP 107790T).

Anoxybacillus vitaminiphilus sp. nov., a strictly aerobic and moderately thermophilic bacterium isolated from a hot spring

2013 ◽  
Vol 63 (Pt_11) ◽  
pp. 4064-4071 ◽  
Author(s):  
Xin-Qi Zhang ◽  
Zhen-Li Zhang ◽  
Nan Wu ◽  
Xu-Fen Zhu ◽  
Min Wu
Keyword(s):  
Hot Spring ◽  
Novel Species ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Unidentified Phospholipid ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Bacterium Strain

A strictly aerobic, Gram-stain-positive, motile and spore-forming bacterium, strain 3nP4T, was isolated from the Puge hot spring located in the south-western geothermal area of China. Strain 3nP4T grew at 38–66 °C (optimum 57–60 °C), at pH 6.0–9.3 (optimum 7.0–7.5) and with 0–4 % (w/v) NaCl (optimum 0–0.5 %). Phylogenetic analysis of 16S rRNA gene sequences, as well as DNA–DNA relatedness values, indicated that the isolate represents a novel species of the genus Anoxybacillus , related most closely to Anoxybacillus voinovskiensis DSM 12111T. Strain 3nP4T had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid as major polar lipids and iso-C15 : 0 and iso-C17 : 0 as major fatty acids, which are both typical chemotaxonomic characteristics of the genus Anoxybacillus . The mean DNA G+C content of strain 3nP4T was 39.2±0.95 mol% (HPLC). A distinctive characteristic of the novel isolate was its extreme reliance on vitamin mixture or yeast extract for growth. Based on data from this taxonomic study using a polyphasic approach, strain 3nP4T is considered to represent a novel species of the genus Anoxybacillus , for which the name Anoxybacillus vitaminiphilus sp. nov. is proposed. The type strain is 3nP4T ( = CGMCC 1.8979T = JCM 16594T).

Purification and characterization of fumarase from the syntrophic propionate-oxidizing bacterium strain MPOB

1996 ◽  
Vol 165 (2) ◽  
pp. 126-131 ◽  
Author(s):  
Bernardina L. M. Van Kuijk ◽  
Nico-Dirk Van Loo ◽  
Alexander F. Arendsen ◽  
Wilfred R. Hagen ◽  
A. J. M. Stams
Keyword(s):  
Purification And Characterization ◽  
Bacterium Strain

scholarly journals Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales

2006 ◽  
Vol 56 (2) ◽  
pp. 369-372 ◽  
Author(s):  
A. I. Slobodkin ◽  
T. P. Tourova ◽  
N. A. Kostrikina ◽  
A. M. Lysenko ◽  
K. E. German ◽  
...  
Keyword(s):  
Hot Spring ◽  
Phylogenetic Analyses ◽  
Casein Hydrolysate ◽  
Rrna Gene ◽  
Donor Strain ◽  
Moderately Thermophilic ◽  
Physiological Properties ◽  
Bacterium Strain ◽  
Casamino Acids ◽  
Ph Range

A moderately thermophilic, anaerobic bacterium (strain SB91T) was isolated from a freshwater hot spring at Barguzin Valley, Buryatiya, Russia. Cells of strain SB91T were straight to slightly curved rods, 0·5–0·6 μm in diameter and 3·0–7·0 μm in length. Formation of endospores was not observed. The temperature range for growth was 26–62 °C, with an optimum at 50 °C. The pH range for growth was 5·5–9·5, with an optimum at pH 7·5–8·0. The substrates utilized by strain SB91T in the presence of 9,10-anthraquinone 2,6-disulfonate included peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, alanine plus glycine, alanine plus proline, l-valine and n-propanol. Carbohydrates were not utilized. Strain SB91T reduced amorphous Fe(III) oxide, Fe(III) citrate, Fe(III) EDTA or Fe(III) nitrilotriacetate with peptone, l-valine or n-propanol as an electron donor. Strain SB91T reduced 9,10-anthraquinone 2,6-disulfonate, thiosulfate, elemental sulfur, fumarate and selenite. Strain SB91T survived after exposure to gamma-radiation at a dose of 5·4 kGy. The G+C content of the DNA of strain SB91T was 33 mol%. Analysis of the 16S rRNA gene sequence revealed that the isolated organism belonged to cluster XII of the clostridia. On the basis of its physiological properties and the results of phylogenetic analyses, it is proposed that strain SB91T represents the sole species of a novel genus, Tepidimicrobium; the name Tepidimicrobium ferriphilum gen. nov., sp. nov. is proposed, with strain SB91T (=DSM 16624T=VKM B-2348T) as the type strain.

A new iron oxidase from a moderately thermophilic iron oxidizing bacterium strain TI-1

2001 ◽  
Vol 268 (6) ◽  
pp. 1653-1658
Author(s):  
Masaki Takai ◽  
Kazuo Kamimura ◽  
Tsuyoshi Sugio
Keyword(s):  
Moderately Thermophilic ◽  
Bacterium Strain

scholarly journals Desulfotomaculum carboxydivorans sp. nov., a novel sulfate-reducing bacterium capable of growth at 100 % CO

2005 ◽  
Vol 55 (5) ◽  
pp. 2159-2165 ◽  
Author(s):  
Sofiya N. Parshina ◽  
Jan Sipma ◽  
Yutaka Nakashimada ◽  
Anne Meint Henstra ◽  
Hauke Smidt ◽  
...  
Keyword(s):  
Gas Phase ◽  
Paper Mill ◽  
Rrna Gene ◽  
Moderately Thermophilic ◽  
Sulfate Reducing ◽  
Nacl Concentration ◽  
Bacterium Strain ◽  
Ph Range ◽  
Desulfotomaculum Nigrificans ◽  
Paper Mill Wastewater

A moderately thermophilic, anaerobic, chemolithoheterotrophic, sulfate-reducing bacterium, strain CO-1-SRBT, was isolated from sludge from an anaerobic bioreactor treating paper mill wastewater. Cells were Gram-positive, motile, spore-forming rods. The temperature range for growth was 30–68 °C, with an optimum at 55 °C. The NaCl concentration range for growth was 0–17 g l−1; there was no change in growth rate until the NaCl concentration reached 8 g l−1. The pH range for growth was 6·0–8·0, with an optimum of 6·8–7·2. The bacterium could grow with 100 % CO in the gas phase. With sulfate, CO was converted to H2 and CO2 and part of the H2 was used for sulfate reduction; without sulfate, CO was completely converted to H2 and CO2. With sulfate, strain CO-1-SRBT utilized H2/CO2, pyruvate, glucose, fructose, maltose, lactate, serine, alanine, ethanol and glycerol. The strain fermented pyruvate, lactate, glucose and fructose. Yeast extract was necessary for growth. Sulfate, thiosulfate and sulfite were used as electron acceptors, whereas elemental sulfur and nitrate were not. A phylogenetic analysis of 16S rRNA gene sequences placed strain CO-1-SRBT in the genus Desulfotomaculum, closely resembling Desulfotomaculum nigrificans DSM 574T and Desulfotomaculum sp. RHT-3 (99 and 100 % similarity, respectively). However, the latter strains were completely inhibited above 20 and 50 % CO in the gas phase, respectively, and were unable to ferment CO, lactate or glucose in the absence of sulfate. DNA–DNA hybridization of strain CO-1-SRBT with D. nigrificans and Desulfotomaculum sp. RHT-3 showed 53 and 60 % relatedness, respectively. On the basis of phylogenetic and physiological features, it is suggested that strain CO-1-SRBT represents a novel species within the genus Desulfotomaculum, for which the name Desulfotomaculum carboxydivorans is proposed. This is the first description of a sulfate-reducing micro-organism that is capable of growth under an atmosphere of pure CO with and without sulfate. The type strain is CO-1-SRBT (=DSM 14880T=VKM B-2319T).

scholarly journals Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste

2007 ◽  
Vol 57 (4) ◽  
pp. 741-744 ◽  
Author(s):  
Chiu-Chung Young ◽  
Peter Kämpfer ◽  
Wen-Ming Chen ◽  
Wen-Shao Yen ◽  
A. B. Arun ◽  
...  
Keyword(s):  
Food Waste ◽  
Type Strain ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Physiological Data ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Moderately Thermophilic ◽  
Gene Sequence Analysis ◽  
Bacterium Strain

A yellow-pigmented, Gram-negative, rod-shaped bacterium (strain CC-YY255T) was isolated from compost generated from food waste collected from Kinmen County, Taiwan. 16S rRNA gene sequence analysis indicated that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Luteimonas; its closest neighbour was the type strain of Luteimonas mephitis (94.4 % sequence similarity). The isolate was distinguished from Luteimonas mephitis on the basis of several phenotypic properties. The organism utilized glucose, maltose, gentiobiose, melibiose and turanose and only a few organic acids (acetate, propionate) and amino acids (l-alanyl glycine, glycyl l-aspartic acid and glycyl l-glutamic acid) as substrates. The fatty acid profile was slightly different from that reported for Luteimonas mephitis. It is evident from the genotypic, chemotaxonomic and physiological data presented that strain CC-YY255T represents a novel species of the genus Luteimonas, for which the name Luteimonas composti sp. nov. is proposed. The type strain is CC-YY255T (=CCUG 53595T=CIP 109311T=BCRC 17598T).

scholarly journals Purification and Characterization of Sulfur Reductase from a Moderately Thermophilic Bacterial Strain, TI-1, that Oxidizes Iron

1998 ◽  
Vol 62 (4) ◽  
pp. 705-709 ◽  
Author(s):  
Tsuyoshi SUGIO ◽  
Keiichi ODA ◽  
Keiko MATSUMOTO ◽  
Masaki TAKAI ◽  
Satoshi WAKASA ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Purification And Characterization ◽  
Moderately Thermophilic ◽  
Sulfur Reductase
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