scholarly journals Identification of the Essential Amino Acid Residues in LukS for the Hemolytic Activity of Staphylococcal Leukocidin towards Rabbit Erythrocytes

1997 ◽  
Vol 61 (12) ◽  
pp. 2095-2099 ◽  
Author(s):  
Hirofumi Nariya ◽  
Akiko Shimatani ◽  
Toshio Tomita ◽  
Yoshiyuki Kamio
Keyword(s):  
Amino Acid ◽  
Hemolytic Activity ◽  
Essential Amino Acid ◽  
Amino Acid Residues

scholarly journals Development of Antimicrobial Stapled Peptides Based on Magainin 2 Sequence

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 444
Author(s):  
Motoharu Hirano ◽  
Chihiro Saito ◽  
Hidetomo Yokoo ◽  
Chihiro Goto ◽  
Ryuji Kawano ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Amino Acid ◽  
Hemolytic Activity ◽  
Helical Structure ◽  
Antimicrobial Activities ◽  
Side Chain ◽  
Membrane Disruption ◽  
Amino Acid Residues ◽  
Electrophysiological Measurements ◽  
Cell Membrane Disruption

Magainin 2 (Mag2), which was isolated from the skin of the African clawed frog, is a representative antimicrobial peptide (AMP) that exerts antimicrobial activity via microbial membrane disruption. It has been reported that the helicity and amphipathicity of Mag2 play important roles in its antimicrobial activity. We investigated and recently reported that 17 amino acid residues of Mag2 are required for its antimicrobial activity, and accordingly developed antimicrobial foldamers containing α,α-disubstituted amino acid residues. In this study, we further designed and synthesized a set of Mag2 derivatives bearing the hydrocarbon stapling side chain for helix stabilization. The preferred secondary structures, antimicrobial activities, and cell-membrane disruption activities of the synthesized peptides were evaluated. Our analyses revealed that hydrocarbon stapling strongly stabilized the helical structure of the peptides and enhanced their antimicrobial activity. Moreover, peptide 2 stapling between the first and fifth position from the N-terminus showed higher antimicrobial activity than that of Mag2 against both gram-positive and gram-negative bacteria without exerting significant hemolytic activity. To investigate the modes of action of tested peptides 2 and 8 in antimicrobial and hemolytic activity, electrophysiological measurements were performed.

scholarly journals Rational design of novel amphipathic antimicrobial peptides focused on the distribution of cationic amino acid residues

MedChemComm ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 896-900 ◽  
Author(s):  
Takashi Misawa ◽  
Chihiro Goto ◽  
Norihito Shibata ◽  
Motoharu Hirano ◽  
Yutaka Kikuchi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Amino Acid ◽  
Human Cell ◽  
Hemolytic Activity ◽  
Rational Design ◽  
Cell Cytotoxicity ◽  
Amino Acid Residues ◽  
High Antimicrobial Activity ◽  
Cationic Amino Acid ◽  
Helical Peptide

Amphipathic helical peptideStripeshowed high antimicrobial activity, low hemolytic activity, and low human cell cytotoxicity.

Analysis of essential amino acid residues for catalytic activity of cis-epoxysuccinate hydrolase from Bordetella sp. BK-52

2013 ◽  
Vol 98 (4) ◽  
pp. 1641-1649 ◽  
Author(s):  
Wenna Bao ◽  
Haifeng Pan ◽  
Zhenhong Zhang ◽  
Yongqing Cheng ◽  
Zhipeng Xie ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Amino Acid Residues

Analysis of essential amino acid residues for catalytic activity of glutaminase from Micrococcus luteus K-3

2006 ◽  
Vol 102 (4) ◽  
pp. 362-364 ◽  
Author(s):  
Shigekazu Yano ◽  
Aki Kamemura ◽  
Kazuaki Yoshimune ◽  
Mitsuaki Moriguchi ◽  
Sachiko Yamamoto ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Micrococcus Luteus ◽  
Amino Acid Residues
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