Purification and Characterization of Konjac Glucomannan Degrading Enzyme from Anaerobic Human Intestinal Bacterium,Clostridium butyricum–Clostvidium beijerinckiiGroup

1997 ◽  
Vol 61 (10) ◽  
pp. 1739-1742 ◽  
Author(s):  
Nobuyoshi Nakajima ◽  
Yasushi Matsuura
2002 ◽  
Vol 48 (3) ◽  
pp. 212-218 ◽  
Author(s):  
M B Martel ◽  
C Hervé du Penhoat ◽  
R Létoublon ◽  
M Fèvre

Among the lytic enzymes secreted by the phytopathogen fungus Sclerotinia sclerotiorum, a starch-degrading enzyme has been isolated and characterized. This glycoprotein of 72 kDa is composed of several isoforms ranging from pI 4.8 to 5.4. The enzymatic parameters have been determined. Specificity studies together with the analysis of the reaction products show that it is an α-1,4-glucanohydrolase. This result is also corroborated by the analysis of the N-terminal and two inner amino acids sequences that are very similar to fungal glucoamylase genes or enzymes so far sequenced.Key words: hydrolase, glucoamylase, Sclerotinia sclerotiorum, starch-degrading enzyme.


1991 ◽  
Vol 71 (5) ◽  
pp. 313-317 ◽  
Author(s):  
Takushi Hatano ◽  
Mutsuko Kosaka ◽  
Zhifeng Cui ◽  
Mieko Kawaguchi ◽  
Tokichi Miyakawa ◽  
...  

Endocrinology ◽  
1981 ◽  
Vol 108 (4) ◽  
pp. 1527-1532 ◽  
Author(s):  
KOICHI YOKONO ◽  
YOSHIMICHI IMAMURA ◽  
KOZUI SHII ◽  
HIDEYO SAKAI ◽  
SHIGEAKI BABA

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