Posttranscriptional Regulation by the Upstream Open Reading Frame of the PhosphoethanolamineN-Methyltransferase Gene

2006 ◽  
Vol 70 (9) ◽  
pp. 2330-2334 ◽  
Author(s):  
Tomoki TABUCHI ◽  
Tomoyuki OKADA ◽  
Tetsushi AZUMA ◽  
Takashi NANMORI ◽  
Takeshi YASUDA
Keyword(s):  
Posttranscriptional Regulation ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame ◽  
Methyltransferase Gene

scholarly journals An upstream open reading frame represses translation of chicken PPARγ transcript variant 1

2019 ◽  
Author(s):  
Yankai Chu ◽  
Jiaxin Huang ◽  
Guangwei Ma ◽  
Tingting Cui ◽  
Xiaohong Yan ◽  
...  
Keyword(s):  
Mrna Stability ◽  
Posttranscriptional Regulation ◽  
Promoter Activity ◽  
Underlying Mechanism ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Reading Frame

AbstractPeroxisome proliferator-activated receptor γ (PPARγ) is a master regulator of adipogenesis. The PPARγ gene produces various transcripts with different 5′-untranslated regions (5′ UTRs) because of alternative promoter usage and splicing. The 5′ UTR plays important roles in posttranscriptional gene regulation. However, to date, the regulatory role and underlying mechanism of 5′ UTRs in the posttranscriptional regulation of PPARγ expression remain largely unclear. In this study, we investigated the effects of 5′ UTRs on posttranscriptional regulation using reporter assays. Our results showed that the five PPARγ 5′ UTRs exerted different effects on reporter gene activity. Bioinformatics analysis showed that chicken PPARγ transcript 1 (PPARγ1) possessed an upstream open reading frame (uORF) in its 5′ UTR. Mutation analysis showed that a mutation in the uORF led to increased Renilla luciferase activity and PPARγ protein expression, but decreased Renilla luciferase and PPARγ1 mRNA expression. mRNA stability analysis using real-time RT-PCR showed that the uORF mutation did not interfere with mRNA stability, but promoter activity analysis of the cloned 5′ UTR showed that the uORF mutation reduced promoter activity. Furthermore, in vitro transcription/translation assays demonstrated that the uORF mutation markedly increased the translation of PPARγ1 mRNA. Collectively, our results indicate that the uORF represses the translation of chicken PPARγ1 mRNA.

scholarly journals Sucrose Control of Translation Mediated by an Upstream Open Reading Frame-Encoded Peptide

2009 ◽  
Vol 150 (3) ◽  
pp. 1356-1367 ◽  
Author(s):  
Fatemeh Rahmani ◽  
Maureen Hummel ◽  
Jolanda Schuurmans ◽  
Anika Wiese-Klinkenberg ◽  
Sjef Smeekens ◽  
...  
Keyword(s):  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame

scholarly journals Translational regulation of Arabidopsis XIPOTL1 is modulated by phosphocholine levels via the phylogenetically conserved upstream open reading frame 30

2012 ◽  
Vol 63 (14) ◽  
pp. 5203-5221 ◽  
Author(s):  
Fulgencio Alatorre-Cobos ◽  
Alfredo Cruz-Ramírez ◽  
Celine A. Hayden ◽  
Claudia-Anahí Pérez-Torres ◽  
Anne-Laure Chauvin ◽  
...  
Keyword(s):  
Translational Regulation ◽  
Open Reading Frame ◽  
Upstream Open Reading Frame ◽  
Reading Frame
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