A Rapid Method for Protein Extraction from Fission Yeast

Yuzy MATSUO; Kazuhide ASAKAWA; Takashi TODA; Satoshi KATAYAMA

doi:10.1271/bbb.60087

A Rapid Method for Protein Extraction from Fission Yeast

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.60087 ◽

2006 ◽

Vol 70 (8) ◽

pp. 1992-1994 ◽

Cited By ~ 47

Author(s):

Yuzy MATSUO ◽

Kazuhide ASAKAWA ◽

Takashi TODA ◽

Satoshi KATAYAMA

Keyword(s):

Fission Yeast ◽

Rapid Method ◽

Protein Extraction

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A Lallzyme MMX-based rapid method for fission yeast protoplast preparation

Yeast ◽

10.1002/yea.2994 ◽

2013 ◽

Vol 31 (2) ◽

pp. 61-66 ◽

Cited By ~ 12

Author(s):

Ignacio Flor-Parra ◽

Jacob Zhurinsky ◽

Manuel Bernal ◽

Paola Gallardo ◽

Rafael R. Daga

Keyword(s):

Fission Yeast ◽

Rapid Method ◽

Yeast Protoplast ◽

Protoplast Preparation

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Texture measurements in Al2O3 using BEKP

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100170773 ◽

1994 ◽

Vol 52 ◽

pp. 608-609

Author(s):

M. D. Vaudin ◽

J. P. Cline

Keyword(s):

Neutron Diffraction ◽

Rapid Method ◽

Crystallographic Orientation ◽

Specimen Surface ◽

X Ray Diffraction ◽

Anisotropic Crystal ◽

X Ray ◽

Verification Method ◽

Crystal Properties ◽

Backscattered Electron

The study of preferred crystallographic orientation (texture) in ceramics is assuming greater importance as their anisotropic crystal properties are being used to advantage in an increasing number of applications. The quantification of texture by a reliable and rapid method is required. Analysis of backscattered electron Kikuchi patterns (BEKPs) can be used to provide the crystallographic orientation of as many grains as time and resources allow. The technique is relatively slow, particularly for noncubic materials, but the data are more accurate than any comparable technique when a sufficient number of grains are analyzed. Thus, BEKP is well-suited as a verification method for data obtained in faster ways, such as x-ray or neutron diffraction. We have compared texture data obtained using BEKP, x-ray diffraction and neutron diffraction. Alumina specimens displaying differing levels of axisymmetric (0001) texture normal to the specimen surface were investigated.BEKP patterns were obtained from about a hundred grains selected at random in each specimen.

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