Effect of Prey Fish Lipids on the Docosahexaenoic Acid Content of Total Fatty Acids in the Lipid ofThunnus albacaresYellowfin Tuna

1996 ◽  
Vol 60 (6) ◽  
pp. 962-965 ◽  
Author(s):  
Hiroaki Saito ◽  
Kenji Ishihara ◽  
Teruaki Murase
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Acid Content ◽  
Prey Fish ◽  
Fish Lipids ◽  
Total Fatty Acids

scholarly journals Development of Growth Media from Agricultural By-Products for Cultivation of PUFA-Producing Sicyoidochytrium minutum

Marine Drugs ◽  
2021 ◽  
Vol 20 (1) ◽  
pp. 8
Author(s):  
Heiðrún Eiríksdóttir ◽  
Magnús Örn Stefánsson ◽  
Hjörleifur Einarsson
Keyword(s):  
Fatty Acids ◽  
Carbon Source ◽  
Docosahexaenoic Acid ◽  
Basal Medium ◽  
Dry Weight ◽  
Growth Media ◽  
Marine Oils ◽  
Human Diet ◽  
Total Fatty Acids ◽  
By Products

The demand for novel sources of marine oils, which contain polyunsaturated fatty acids (PUFAs), has increased due to the realization of the importance of PUFAs, e.g., docosahexaenoic acid (DHA), in the human diet. However, the natural supply is limited. By-product peptones (BYPP) intended as a growth medium for the PUFA-producing strain Sicyoidochytrium minutum of family Thraustochytriaceae were produced after several experiments on the pancreatic digestion of bovine lungs and spleens. S. minutum was able to grow in a medium containing BYPP made from the pancreatic digestion of lung and spleen with glycerol, resulting in 1.14 ± 0.03 g cell dry weight (CDW)/L and 1.44 ± 0.24 g CDW/L, respectively, after 5 days of incubation at 25 °C, compared to 1.92 ± 0.25 g CDW/L in Basal Medium (BM) containing tryptone, peptone, and glycerol. The lipid content, obtained after growth in lung BYPP media with glycerol as a carbon source, was significantly higher (28.17% ± 1.33 of dry weight) than in the control basal medium (BM) (21.72% ± 2.45); however, DHA as a percentage of total fatty acids was lower in BYPP than in the control BM (25.24% ± 1.56 and 33.02% ± 2.37, respectively). It is concluded that low-value by-products from abattoirs can be used as ingredients for the cultivation of oligogenic Thraustochytriaceae.

Influence of Simazine and Atrazine on Free Fatty Acid Content in Isolated Chloroplasts

Weed Science ◽  
1973 ◽  
Vol 21 (1) ◽  
pp. 57-60 ◽  
Author(s):  
A. E. Smith ◽  
R. E. Wilkinson
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Free Fatty Acid ◽  
Free Fatty Acid Content ◽  
Acid Content ◽  
Spinacia Oleracea ◽  
Saturated Fatty Acids ◽  
Fatty Acid Content ◽  
Total Fatty Acids ◽  
Isolated Chloroplasts

Chloroplasts, isolated from fresh spinach (Spinacia oleracea L.) were treated with four concentrations of each 2-chloro-4,6-bis(ethylamino)-s-triazine (simazine) or 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine (atrazine) to determine the influence of these herbicides on the free fatty acid content in isolated chloroplasts. The treated chloroplast solutions were sampled at 0, 5, 15, 30, 60, and 120 min following treatment. Results indicated that most herbicide treatments increased free fatty acid content in isolated chloroplasts when compared with the zero concentration treatments. The increased fatty acid content was similar for most concentrations of simazine and atrazine. Results of individual fatty acid analyses indicated that the increase in total fatty acids was a result of increases in concentration of unsaturated rather than saturated fatty acids.

The alpha-linolenic Acid Content of Green Vegetables Commonly Available in Australia

2001 ◽  
Vol 71 (4) ◽  
pp. 223-228 ◽  
Author(s):  
Christine Pereira ◽  
Duo Li ◽  
Andrew J. Sinclair
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linolenic Acid ◽  
Acid Composition ◽  
Acid Content ◽  
Omega 3 ◽  
Alpha Linolenic Acid ◽  
Brussels Sprouts ◽  
Total Fatty Acids

Green vegetable consumption has long been considered to have health benefits mainly due to the vitamins, minerals and phytonutrients (such as vitamin C, folate, antioxidants etc) contained in a vegetable-rich diet. Additionally, green vegetables are known to contain a relatively high proportion of omega-3 polyunsaturated fatty acids (PUFAs), primarily in the form of alpha-linolenic acid (18:3n-3). However, there are no data available on the fatty acid composition and concentration of green vegetables commonly consumed in Australia. The present study determined the fatty acid content of 11 green vegetables that are commonly available in Australia. The total fatty acid concentrations of the vegetables under study ranged from 44 mg/100 g wet weight in Chinese cabbage to 372 mg/100 g in watercress. There were three PUFAs in all vegetables analyzed; these were 16:3n-3, 18:2n-6, and 18:3n-3 fatty acids. Sample vegetables contained significant quantities of 16:3n-3 and 18:3n-3, ranging from 23 to 225 mg/100g. Watercress and mint contained the highest amounts of 16:3n-3 and 18:3n-3, and parsley had the highest amount of 18:2n-6 in both percentage composition and concentration. Mint had the highest concentration of 18:3n-3 with a value of 195 mg/100 g, while watercress contained the highest concentration of 16:3n-3 at 45 mg/100 g. All 11 green vegetables contained a high proportion of PUFAs, ranging from 59 to 72% of total fatty acids. The omega-3 PUFA composition ranged from 40 to 62% of total fatty acids. Monounsaturated fatty acid composition was less than 6% of total fatty acids. The proportion of saturated fatty acids ranged from 21% in watercress and mint to 32% of total fatty acids in Brussels sprouts. No eicosapentaenoic and docosahexaenoic acids were detected in any of the samples. Consumption of green vegetables could contribute to 18:3n-3 PUFA intake, especially for vegetarian populations.

scholarly journals Maternal docosahexaenoic acid supplementation and fetal accretion

2003 ◽  
Vol 90 (1) ◽  
pp. 135-145 ◽  
Author(s):  
Colette Montgomery ◽  
Brian K. Speake ◽  
Alan Cameron ◽  
Naveed Sattar ◽  
Lawrence T. Weaver
Keyword(s):  
Fatty Acids ◽  
Nervous System ◽  
Docosahexaenoic Acid ◽  
Venous Blood ◽  
Double Blind Study ◽  
Double Blind ◽  
Blood Samples ◽  
Total Fatty Acids ◽  
Maternal Supplementation ◽  
Maternal Status

Docosahexaenoic acid (DHA) (22:6n−3) is a polyunsaturated fatty acid that is an essential constituent of membranes, particularly of the nervous system. Infants acquire DHA from their mothers, either prenatally via the placenta or postnatally in milk. The present study aimed to test the hypothesis that maternal supplementation during the second and third trimesters of pregnancy enriches maternal and/or fetal DHA status. In a randomised, prospective, double-blind study 100 mothers received either fish-oil capsules containing 400mg DHA/g (200mg/d) (n 50), or placebo containing 810mg oleic acid/g (400mg/d) (n 50) from 15 weeks gestation until term. Venous blood samples were obtained from mothers at 15, 28 and 40 weeks, and from the umbilical cord at birth. Total fatty acids in plasma and erythrocytes were analysed by GC–MS. There were no significant differences between maternal groups in baseline DHA, as a proportion of total fatty acids (g/100g total fatty acids) or concentration (nmol/ml), in plasma and erythrocytes. DHA concentrations in plasma at 28 weeks (P=0·02) and erythrocytes at both 28 weeks (P=0·03) and term (P=0·02) were 20% higher in supplemented mothers than the placebo group. DHA accounted for a higher proportion of total fatty acids in erythrocytes of supplemented mothers at 28 weeks (P=0·003) and term (P=0·01). There were no significant differences between groups in DHA (g/100g total fatty acids or nmol/l) in cord blood. Maternal DHA status was maximal in mid-trimester and declined to term, at a lower rate in supplemented compared with unsupplemented mothers. Maternal DHA supplementation significantly increases maternal DHA status and limits the last trimester decline in maternal status, aiding preferential transfer of DHA from mother to fetus.

Oils rich in docosahexaenoic acid in livers of sharks from temperate Australian waters

1998 ◽  
Vol 49 (7) ◽  
pp. 763 ◽  
Author(s):  
Peter D. Nichols ◽  
Michael J. Bakes ◽  
Nicholas G. Elliott
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Docosahexaenoic Acid ◽  
Wax Ester ◽  
Shark Species ◽  
Flame Ionization ◽  
Total Fatty Acids ◽  
Wet Weight ◽  
Galeorhinus Galeus ◽  
Direct Use

The livers from the two main commercially-targeted shark species in southern Australia (Mustelus antarcticus, gummy shark; Galeorhinus galeus, school shark), together with Squalus acanthias (white-spotted spurdog), were analysed for oil content and composition, and fatty acid composition. The yield of oil from the liver was 30-64% (wet weight) for M. antarcticus and 50-53% (wet weight) for G. galeus. Lipid classes were determined by thin-layer chromatography with flame ionization detection, with the major lipid being triacylglycerol (≥95%); other minor lipids were polar lipid, wax ester, sterol (mainly cholesterol) and free fatty acid. Long-chain ω-3 polyunsaturated fatty acids accounted for between 33% and 39% of the total fatty acids in all three species, and docosahexaenoic acid (DHA) concentrations were between 13% and 18%. The liver oils of M. antarcticus and G. galeus and other shark species may be an attractive source of w-3 fatty acids, specifically DHA, for direct use and/or for adding further value.

Effect of double-muscling in Belgian Blue young bulls on the intramuscular fatty acid composition with emphasis on conjugated linoleic acid and polyunsaturated fatty acids

2001 ◽  
Vol 73 (2) ◽  
pp. 253-260 ◽  
Author(s):  
K. Raes ◽  
S. de Smet ◽  
D. Demeyer
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Polyunsaturated Fatty Acids ◽  
Acid Composition ◽  
Acid Content ◽  
Fatty Acid Content ◽  
Total Fatty Acid Content ◽  
Total Fatty Acids

AbstractThe effect of double-muscling (DM) genotype (double-muscling, mh/mh; heterozygous, mh/+; normal, +/+) of Belgian Blue (BB) young bulls on the intramuscular fatty acid composition, in particular conjugated linoleic acid (CLA) and polyunsaturated fatty acids (PUFA) was examined in five different muscles. The relative fatty acid composition showed only minor differences between muscles within genotypes. However, the DM genotype had a large effect on both the intramuscular total fatty acid content and on the relative fatty acid composition. Across muscles, the mh/mh animals had a lower total fatty acid content compared with the +/+animals (907 v: 2656 mg/100 g muscle;P< 0·01) and a higher PUFA proportion in total fatty acids (27·5 v 11·3 g/100 g total fatty acids;P< 0001), resulting in a higher PUFA/saturated fatty acid ratio (0·55 v 0·18;P< 0·01) and a lower n-6/n-3 ratio (5·34 v. 6·17;P< 0·01). The heterozygous genotype was intermediate between the two homozygous genotypes. The relative CLA content was similar in the mh/mh and +/+ genotypes and approximated 0·4 to 0·5 g/100 g total fatty acids. From the data it is further suggested that differences in the metabolism of the n-3 and n-6 fatty acids could exist between DM genotypes.

scholarly journals Cultivation Method Effect on Schizochytrium sp. Biomass Growth and Docosahexaenoic Acid (DHA) Production with the Use of Waste Glycerol as a Source of Organic Carbon

Energies ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2952
Author(s):  
Natalia Kujawska ◽  
Szymon Talbierz ◽  
Marcin Dębowski ◽  
Joanna Kazimierowicz ◽  
Marcin Zieliński
Keyword(s):  
Fatty Acids ◽  
Docosahexaenoic Acid ◽  
Batch Culture ◽  
Acid Production ◽  
Carbon Sources ◽  
Fed Batch ◽  
Waste Glycerol ◽  
Total Fatty Acids ◽  
Fed Batch Culture ◽  
Dha Production

Inexpensive carbon sources offering an alternative to glucose are searched for to reduce costs of docosahexaenoic acid production by microalgae. The use of waste glycerol seems substantiated and prospective in this case. The objective of this study was to determine the production yield of heterotrophic microalgae Schizochytrium sp. biomass and the efficiency of docosahexaenoic acid production in various types of cultures with waste glycerol. Cultivation conditions were optimized using the Plackett–Burman method and Response Surface Methodology. The highest technological performance was obtained in the fed-batch culture, where the concentration of Schizochytrium sp. biomass reached 103.44 ± 1.50 g/dm3, the lipid concentration in Schizochytrium sp. biomass was at 48.85 ± 0.81 g/dm3, and the docosahexaenoic acid concentration at 21.98 ± 0.36 g/dm3. The highest docosahexaenoic acid content, accounting for 61.76 ± 3.77% of total fatty acids, was determined in lipid bodies of the Schizochytrium sp. biomass produced in the batch culture, whereas the lowest one, accounting for 44.99 ± 2.12% of total fatty acids, in those of the biomass grown in the fed-batch culture.

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