αs1-Casein-specific CD8+T Cell Clone That Inhibits Its Own Interferon-γProduction by Producing Interleukin-10

1995 ◽  
Vol 59 (12) ◽  
pp. 2274-2276 ◽  
Author(s):  
Ken-ichi Nishijima ◽  
Tatsuhiro Hisatsune ◽  
Hiroko Kato ◽  
Osamu Shiho ◽  
Shuichi Kaminogawa
Keyword(s):  
T Cell ◽  
Cell Clone ◽  
Cd8 T Cell ◽  
Interleukin 10 ◽  
T Cell Clone

scholarly journals A porcine CD8+ T Cell Clone with Heterotypic Specificity for Foot-and-mouth Disease Virus

1996 ◽  
Vol 77 (9) ◽  
pp. 2089-2096 ◽  
Author(s):  
A. Rodriguez ◽  
V. Ley ◽  
E. Ortuno ◽  
A. Ezquerra ◽  
A. Saalmuller ◽  
...  
Keyword(s):  
T Cell ◽  
Disease Virus ◽  
Cell Clone ◽  
Foot And Mouth Disease ◽  
Cd8 T Cell ◽  
Mouth Disease ◽  
T Cell Clone ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals IL4I1 Accelerates the Expansion of Effector CD8+ T Cells at the Expense of Memory Precursors by Increasing the Threshold of T-Cell Activation

2020 ◽  
Vol 11 ◽  
Author(s):  
Marie-Line Puiffe ◽  
Aurélie Dupont ◽  
Nouhoum Sako ◽  
Jérôme Gatineau ◽  
José L. Cohen ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
T Cell Activation ◽  
Cd8 T Cells ◽  
Cell Activation ◽  
Cell Clone ◽  
Cd8 T Cell ◽  
Lymphocytic Choriomeningitis ◽  
T Cell Clone

IL4I1 is an immunoregulatory enzyme that inhibits CD8 T-cell proliferation in vitro and in the tumoral context. Here, we dissected the effect of IL4I1 on CD8 T-cell priming by studying the differentiation of a transgenic CD8 T-cell clone and the endogenous repertoire in a mouse model of acute lymphocytic choriomeningitis virus (LCMV) infection. Unexpectedly, we show that IL4I1 accelerates the expansion of functional effector CD8 T cells during the first several days after infection and increases the average affinity of the elicited repertoire, supporting more efficient LCMV clearance in WT mice than IL4I1-deficient mice. Conversely, IL4I1 restrains the differentiation of CD8 T-cells into long-lived memory precursors and favors the memory response to the most immunodominant peptides. IL4I1 expression does not affect the phenotype or antigen-presenting functions of dendritic cells (DCs), but directly reduces the stability of T-DC immune synapses in vitro, thus dampening T-cell activation. Overall, our results support a model in which IL4I1 increases the threshold of T-cell activation, indirectly promoting the priming of high-affinity clones while limiting memory T-cell differentiation.

scholarly journals An expanded parenchymal CD8+ T cell clone in GABA A receptor encephalitis

2020 ◽  
Vol 7 (2) ◽  
pp. 239-244 ◽  
Author(s):  
Aline Bracher ◽  
Carmen Alcalá ◽  
Jaime Ferrer ◽  
Nico Melzer ◽  
Reinhard Hohlfeld ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Clone ◽  
Cd8 T Cell ◽  
Gaba A ◽  
T Cell Clone

scholarly journals Anti-TCR-Specific DNA Vaccination Demonstrates a Role for a CD8+T Cell Clone in the Induction of Allograft Tolerance by Donor-Specific Blood Transfusion

2000 ◽  
Vol 165 (1) ◽  
pp. 96-101 ◽  
Author(s):  
Caroline Vignes ◽  
Elise Chiffoleau ◽  
Patrice Douillard ◽  
Régis Josien ◽  
Hélène Pêche ◽  
...  
Keyword(s):  
T Cell ◽  
Blood Transfusion ◽  
Cell Clone ◽  
Cd8 T Cell ◽  
Dna Vaccination ◽  
T Cell Clone

scholarly journals Cytokine-independent growth and clonal expansion of a primary human CD8+ T-cell clone following retroviral transduction with the IL-15 gene

Blood ◽  
2007 ◽  
Vol 109 (12) ◽  
pp. 5168-5177 ◽  
Author(s):  
Cary Hsu ◽  
Stephanie A. Jones ◽  
Cyrille J. Cohen ◽  
Zhili Zheng ◽  
Keith Kerstann ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cell Clone ◽  
Rare Event ◽  
Cd8 T Cell ◽  
Vector System ◽  
Effector Memory ◽  
Hematopoietic Stem ◽  
Continuous Growth ◽  
T Cell Clone

Abstract Malignancies arising from retrovirally transduced hematopoietic stem cells have been reported in animal models and human gene therapy trials. Whether mature lymphocytes are susceptible to insertional mutagenesis is unknown. We have characterized a primary human CD8+ T-cell clone, which exhibited logarithmic ex vivo growth in the absence of exogenous cytokine support for more than 1 year after transduction with a murine leukemia virus–based vector encoding the T-cell growth factor IL-15. Phenotypically, the clone was CD28−, CD45RA−, CD45RO+, and CD62L−, a profile consistent with effector memory T lymphocytes. After gene transfer with tumor-antigen–specific T-cell receptors, the clone secreted IFN-γ upon encountering tumor targets, providing further evidence that they derived from mature lymphocytes. Gene-expression analyses revealed no evidence of insertional activation of genes flanking the retroviral insertion sites. The clone exhibited constitutive telomerase activity, and the presence of autocrine loop was suggested by impaired cell proliferation following knockdown of IL-15Rα expression. The generation of this cell line suggests that nonphysiologic expression of IL-15 can result in the long-term in vitro growth of mature human T lymphocytes. The cytokine-independent growth of this line was a rare event that has not been observed in other IL-15 vector transduction experiments or with any other integrating vector system. It does not appear that the retroviral vector integration sites played a role in the continuous growth of this cell clone, but this remains under investigation.

Generating of Human CD4+ and CD8+ T Lymphocytes toward NY-ESO-1 by T Cell Receptor (TCR) Modification and Transduction for Adoptive TCR Gene-Transfer

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 3533-3533
Author(s):  
Holger Krönig ◽  
Kathrin Hofer ◽  
Daniel Sommermeyer ◽  
Christian Peschel ◽  
Wolfgang Uckert ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Gene Transfer ◽  
T Cell Receptor ◽  
Cell Clone ◽  
Cd8 T Cell ◽  
Cell Receptor ◽  
Cell Clones ◽  
T Cell Clone ◽  
T Cell Transfer

Abstract The Cancer Testis (CT) antigen NY-ESO-1 is one of the most immunogenic cancer antigens eliciting strong humoral and cellular immune responses in tumor patients and therefore it is a promising candidate antigen for successful adoptive T cell transfer. The aim of our studies is the transfer of autologous T cells re-directed towards CT antigens by T cell receptor (TCR) gene transfer. The first precondition for genetic transfer of CT-Ag-specific TCRs is the availability of tumor-reactive CD4+ and CD8+ T cell clones that express a CT-Ag-specific TCR. Therefore, we generated the autologous CD8+ T cell clone ThP2 through stimulating HLA-A2.1− PBMCs with autologous HLA-A2+DCs loaded with synthetic NY-ESO-1157–165. After two restimulations, FACS-sorting and cloning, the T cell line specifically recognized the NY-ESO-1157–165 peptide and also specifically lysed NY-ESO-1157–165 expressing tumor cells. In addition, we generated NY-ESO-1 specific T helper1 clones from HLA-DR1+ and HLA-DR4+ healthy donors by stimulation of CD4+ T cells with autologous dendritic cells (DC) pulsed with the NY-ESO-187–111 peptide. The specificity of CD4+ T helper cell clones was determined by proliferation assays and IFN gamma ELISPOT through screening with the NY-ESO-187–111 peptide. By limiting dilution of the NYESO- 1-specific T cell populations we succeeded to isolate CD4+ T cell clones, which recognized NY-ESO-1-pulsed target cells and DCs pulsed with NY-ESO-1 protein. The second precondition for TCR gene transfer is the availability of efficient vector systems. Using vectors based upon mouse myelo-proliferative sarcoma virus (MPSV), it was possible to achieve a high transgene expression in the TCR-transduced T cells. Therefore, we cloned the TCR of the HL-A2-restricted NY-ESO-1-specific CTL clone ThP2 in the retroviral vector and documented the correct expression of the TCR-chains using peptide/HLA-multimers following retroviral transduction of peripheral PBMCs. Moreover, the NY-ESO-1 specific lysis of HLA-A2+ NY-ESO-1+ tumor cell lines after transduction in primary T cells was as well effective as the primary T cell clone. Because the expression of naive transgenic T cell receptors in recipient human T cells is often insufficient to achieve highly reactive T cell bulks we modified the TCR of the ThP2 CTL clone by, murinisation, codon optimalization or by introducing cysteins into the constant regions. Afterwards we compared the expression efficiency of the three different modifications on naive T cells by tetramer-staining. We were able to show that codon optimalization leads to an increase in the expression levels of the transgenic TCRs in human CD8+ T cells. The next step is the development of T cell transfer regiments, which are based on class-II-restricted TCR-transduced T cells.

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