Nucleotide Sequence at the 3′-Terminal Region of Sweet Potato Feathery Mottle Virus (Ordinary Strain, SPFMV-O) RNA

1994 ◽  
Vol 58 (5) ◽  
pp. 965-967 ◽  
Author(s):  
Masaki Mori ◽  
Tomio Usugi ◽  
Takaharu Hayashi ◽  
Masamichi Nishiguchi
Keyword(s):  
Nucleotide Sequence ◽  
Sweet Potato ◽  
Terminal Region ◽  
Mottle Virus ◽  
Ordinary Strain

scholarly journals First Report of Sweet potato chlorotic stunt virus and Sweet potato feathery mottle virus Infecting Sweet Potato in Spain

Plant Disease ◽  
2004 ◽  
Vol 88 (4) ◽  
pp. 428-428 ◽  
Author(s):  
R. A. Valverde ◽  
G. Lozano ◽  
J. Navas-Castillo ◽  
A. Ramos ◽  
F. Valdés
Keyword(s):  
Nucleotide Sequence ◽  
Sweet Potato ◽  
Morning Glory ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Degenerate Primers ◽  
Potato Plants ◽  
Elisa Testing ◽  
Two Samples

Sweet potato chlorotic stunt virus (SPCSV), family Closteroviridae and Sweet potato feathery mottle virus (SPFMV), family Potyviridae are whitefly and aphid transmitted, respectively, which in double infections cause sweet potato virus disease (SPVD) that is a serious sweet potato (Ipomoea batatas Lam.) disease in Africa (2). During the past decade, sweet potato plants showing symptoms similar to SPVD have been observed in most areas of Spain. Nevertheless, not much information is available about the identity of the viruses infecting this crop in Spain. During the summer of 2002, sweet potato plants with foliar mosaic, stunting, leaf malformation, chlorosis, and ringspot symptoms were observed in several farms in Málaga (southern Spain) and Tenerife and Lanzarote (Canary Islands, Spain). Vine cuttings were collected from 21 symptomatic plants in Málaga and from eight plants on Lanzarote and six on Tenerife. Scions were grafted to the indicator hosts, Brazilian morning glory (I. setosa) and I. nil cv. Scarlett O'Hara. Three weeks after graft inoculations, all plants showed various degrees of mosaic, chlorosis, leaf malformation, and stunting. Four field collections (two from Málaga, one from Tenerife, and one from Lanzarote) with severe symptoms on I. setosa were selected for whitefly (Bemisia tabaci biotype Q) transmission experiments. Acquisition and transmission periods were 48 h. I. setosa was the acquisition host, and I. nil was the transmission host. For each isolate, groups of 10 whiteflies per I. nil plant were used. All I. nil plants used as transmission hosts with the four, field collections showed chlorosis and leaf malformation. Reverse-transcription polymerase chain reaction (RT-PCR) was performed on I. setosa (grafted with the four selected field collections) and I. nil plants (from the whitefly transmission experiments) with primers for the HSP70h gene of SPCSV. A 450-bp DNA fragment was obtained with all I. setosa and I. nil samples. Sequencing of the 450-bp DNA from two samples from Málaga yielded a nucleotide sequence with 98 to 99% similarity to the HSP70h gene of West African SPCSV isolates. Foliar samples from I. setosa, originally grafted with the 21 vine cuttings, were used for nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA) testing with antiserum specific to SPFMV-RC (provided by J. Moyer, North Carolina State University, Raleigh). Positive control was sap extract from I. setosa that was infected with the common strain of SPFMV. Procedures for NCM-ELISA were as described (4). NCM-ELISA testing suggested that SPFMV was present in all samples. RT-PCR was conducted with degenerate primers POT1/POT2 (1). The nucleotide sequence that was amplified by these two primers spans part of the NIb protein and part of the coat protein gene of potyviruses. All samples yielded the expected 1.3-kb DNA. Sequencing of the RT-PCR products of two isolates from Malaga and sequence comparisons yielded nucleotide sequences with 97% similarity to two East African isolates (Nam 1 and Nam 3) of SPFMV (3). These results confirm the presence of SPCSV and SPFMV in sweet potato in Spain. References: (1) D. Colinet and J. Kummert. J. Virol. Methods 45:149, 1993. (2) R. W. Gibson et al. Plant Pathol. 47:95, 1998. (3) J. F. Kreuze et al. Arch. Virol. 145:567, 2000. (4) E. R. Souto et al. Plant Dis. 87:1226, 2003.

Nucleotide Sequence of the 3′ -terminal Region of Carnation vein mottle virus RNA

2000 ◽  
Vol 66 (3) ◽  
pp. 251-253 ◽  
Author(s):  
Takahide SASAYA ◽  
Gabriela DUJOVNY ◽  
Hiroki KOGANEZAWA
Keyword(s):  
Nucleotide Sequence ◽  
Terminal Region ◽  
Mottle Virus ◽  
Virus Rna

scholarly journals Incidence and distribution of Sweetpotato viruses and their implication on sweetpotato seed system in Malawi

2021 ◽  
Author(s):  
Willard Mbewe ◽  
Andrew Mtonga ◽  
Margret Chiipanthenga ◽  
Kennedy Masamba ◽  
Gloria Chitedze ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Virus Disease ◽  
Disease Incidence ◽  
Mottle Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Foliar Symptoms ◽  
Membrane Enzyme ◽  
Sweet Potato Virus ◽  
Seed System ◽  
Clean Seed

AbstractA survey was carried out in 19 districts to investigate the prevalence and distribution of sweetpotato virus disease (SPVD) and its implication on the sustainability of clean seed system in Malawi. A total of 166 leaf samples were collected and tested for the presence of 8 viruses using nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). SPVD foliar symptoms were observed in 68.42% of the surveyed districts. There were significant variations in disease incidence and severity (p < 0.001) among districts, with the highest incidence in Mulanje (28.34%). Average SPVD severity score was 3.05. NCM-ELISA detected sweet potato feathery mottle virus (SPFMV, 30.54%), sweet potato mild mottle virus (SPMMV, 31.14%), sweet potato mild speckling virus (SPMSV, 16.17%), sweet potato C-6 virus (SPC6V, 13.77%), sweet potato chlorotic stunt virus (SPCSV, 22.16%), sweet potato collusive virus (SPCV, 30.54%), sweet potato virus G (SPVG, 11.38%), cucumber mosaic virus (CMV, 7.78%) either in single or mixed infections. Data from this study indicate a significant SPVD occurrence in the country, and the consequence implications towards national sweetpotato seed system.

scholarly journals Strawberry Mottle Virus (Family Secoviridae, Order Picornavirales) Encodes a Novel Glutamic Protease To Process the RNA2 Polyprotein at Two Cleavage Sites

2018 ◽  
Vol 93 (5) ◽  
Author(s):  
Krin S. Mann ◽  
Joan Chisholm ◽  
Hélène Sanfaçon
Keyword(s):  
Cysteine Proteases ◽  
Terminal Region ◽  
Mottle Virus ◽  
Cleavage Sites ◽  
Virus Family ◽  
Positive Strand ◽  
Viral Proteases ◽  
Bipartite Genome ◽  
The Family ◽  
Positive Strand Rna

ABSTRACT Strawberry mottle virus (SMoV) belongs to the family Secoviridae (order Picornavirales) and has a bipartite genome with each RNA encoding one polyprotein. All characterized secovirids encode a single protease related to the picornavirus 3C protease. The SMoV 3C-like protease was previously shown to cut the RNA2 polyprotein (P2) at a single site between the predicted movement protein and coat protein (CP) domains. However, the SMoV P2 polyprotein includes an extended C-terminal region with a coding capacity of up to 70 kDa downstream of the presumed CP domain, an unusual characteristic for this family. In this study, we identified a novel cleavage event at a P↓AFP sequence immediately downstream of the CP domain. Following deletion of the PAFP sequence, the polyprotein was processed at or near a related PKFP sequence 40 kDa further downstream, defining two protein domains in the C-terminal region of the P2 polyprotein. Both processing events were dependent on a novel protease domain located between the two cleavage sites. Mutagenesis of amino acids that are conserved among isolates of SMoV and of the related Black raspberry necrosis virus did not identify essential cysteine, serine, or histidine residues, suggesting that the RNA2-encoded SMoV protease is not related to serine or cysteine proteases of other picorna-like viruses. Rather, two highly conserved glutamic acid residues spaced by 82 residues were found to be strictly required for protease activity. We conclude that the processing of SMoV polyproteins requires two viral proteases, the RNA1-encoded 3C-like protease and a novel glutamic protease encoded by RNA2. IMPORTANCE Many viruses encode proteases to release mature proteins and intermediate polyproteins from viral polyproteins. Polyprotein processing allows regulation of the accumulation and activity of viral proteins. Many viral proteases also cleave host factors to facilitate virus infection. Thus, viral proteases are key virulence factors. To date, viruses with a positive-strand RNA genome are only known to encode cysteine or serine proteases, most of which are related to the cellular papain, trypsin, or chymotrypsin proteases. Here, we characterize the first glutamic protease encoded by a plant virus or by a positive-strand RNA virus. The novel glutamic protease is unique to a few members of the family Secoviridae, suggesting that it is a recent acquisition in the evolution of this family. The protease does not resemble known cellular proteases. Rather, it is predicted to share structural similarities with a family of fungal and bacterial glutamic proteases that adopt a lectin fold.

scholarly journals Genetic Diversity of Sweet potato feathery mottle virus from Sweet Potatoes in Korea

2007 ◽  
Vol 23 (1) ◽  
pp. 13-21 ◽  
Author(s):  
Hae-Ryun Kwak ◽  
Mi-Kyeong Kim ◽  
Mi-Nam Jung ◽  
Su-Heon Lee ◽  
Jin-Woo Park ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Mottle Virus ◽  
Sweet Potatoes

scholarly journals Nucleotide sequence variation associated with β-amylase deficiency in the sweet potato Ipomoea batatas (L.) Lam

2009 ◽  
Vol 59 (3) ◽  
pp. 209-216 ◽  
Author(s):  
Nadia Anwar ◽  
Akira Kikuchi ◽  
Toru Kumagai ◽  
Kazuo N. Watanabe
Keyword(s):  
Nucleotide Sequence ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Sequence Variation ◽  
Nucleotide Sequence Variation

Nucleotide sequence of the 3′ terminal region of the genome of four Lettuce mosaic virus isolates from Greece and Yemen

1999 ◽  
Vol 144 (8) ◽  
pp. 1619-1626 ◽  
Author(s):  
F. Revers ◽  
R. A. A. van der Vlugt ◽  
S. Souche ◽  
M. Lanneau ◽  
H. Lot ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Terminal Region ◽  
Lettuce Mosaic Virus ◽  
Virus Isolates
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