scholarly journals Enzymatic Functions of Fatty Acid-modified Chymotrypsin and Trypsin in Aqueous-organic Media

1994 ◽  
Vol 58 (3) ◽  
pp. 512-516 ◽  
Author(s):  
Yoshihiro Kawasaki ◽  
Yoshinori Takahashi ◽  
Masaru Nakatani ◽  
Mototake Murakami ◽  
Shun’ichi Dosako ◽  
...  
Keyword(s):  
1994 ◽  
Vol 58 (6) ◽  
pp. 1162-1163 ◽  
Author(s):  
Yoshihiro Kawasaki ◽  
Yoshinori Takahashi ◽  
Masaru Nakatani ◽  
Mototake Murakami ◽  
Shun’ichi Dosako ◽  
...  

2002 ◽  
Vol 92 (3) ◽  
pp. 259-266 ◽  
Author(s):  
Mohamed Gargouri ◽  
Philippe Drouet ◽  
Marie-Dominique Legoy

2020 ◽  
Vol 69 (7) ◽  
pp. 737-742
Author(s):  
Serap Çetinkaya ◽  
Ali Fazıl Yenidünya ◽  
Faika Başoğlu ◽  
Kamuran Saraç

2006 ◽  
Vol 12 (3) ◽  
pp. 147-151 ◽  
Author(s):  
Sasa Sabeder ◽  
Maja Habulin ◽  
Zeljko Knez

Sugar fatty acid esters are biodegradable surface active compounds in foodstuffs and cosmetics or Pharmaceuticals. They have potential in replacing pollutant chemically synthesized surfactants. The enzymatic synthesis of fructose palmitate catalyzed by Candida antarctica B lipase was performed in different organic media in a batch reactor at atmospheric pressure. The influence of the organic solvent and temperature on the esterification was studied. Since supercritical carbon dioxide (SC CO2) has several advantages over organic solvents, such as high reaction rate, high mass transfer n?n-toxicity, non-flammability and low price, it was also chosen as a reaction medium for fructose palmitate production. The influence of temperature on immobilized lipase activity was studied at 10 MPa and the results were compared to the results obtained from reactions performed at atmospheric pressure under the same reaction conditions. The highest conversion (67%) was obtained after 24 hours of reaction in SC CO2 at 80?C. A change of the particle size distribution and morphology of the untreated lipase and lipase treated with 2-methyl 2-butanol and SC ??2 was observed.


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