scholarly journals Folimycin (concanamycin A) and Bafilomycin A1, Inhibitors Specific for V-ATPase, Exert Similar but Distinct Effects on Intracellular Translocation and Processing of Glycoproteins

1994 ◽  
Vol 58 (2) ◽  
pp. 425-427 ◽  
Author(s):  
Makoto Muroi ◽  
Nobue Shiragami ◽  
Kenji Nagao ◽  
Makari Yamasaki ◽  
Akira Takatsuki
Keyword(s):  
Bafilomycin A1 ◽  
Concanamycin A ◽  
Intracellular Translocation

Important role for the V-type H+-ATPase and the Golgi apparatus in the recycling of PTH/PTHrP receptor

2004 ◽  
Vol 286 (5) ◽  
pp. E704-E710 ◽  
Author(s):  
Hesham A. W. Tawfeek ◽  
Abdul B. Abou-Samra
Keyword(s):  
Golgi Apparatus ◽  
Fluorescent Protein ◽  
Brefeldin A ◽  
Receptor Recycling ◽  
Bafilomycin A1 ◽  
Coated Pits ◽  
Concanamycin A ◽  
Hydrogen Atpase ◽  
Green Fluorescent ◽  
Pthrp Receptor

Our previous studies demonstrated that a green fluorescent protein-tagged parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor stably expressed in LLCPK-1 cells undergoes agonist-dependent internalization into clathrin-coated pits. The subcellular localization of the internalized PTH/PTHrP receptor is not known. In the present study, we explored the intracellular pathways of the internalized PTH/PTHrP receptor. Using immunofluorescence and confocal microscopy, we show that the internalized receptors localize at a juxtanuclear compartment identified as the Golgi apparatus. The receptors do not colocalize with lysosomes. Furthermore, whereas the internalized receptors exhibit rapid recycling, treatment with proton pump inhibitors (bafilomycin-A1 and concanamycin A) or brefeldin A, Golgi disrupting agents, reduces PTH/PTHrP receptor recycling. Together, these data indicate an important role for the vacuolar-type hydrogen-ATPase and the Golgi apparatus in postendocytic PTH/PTHrP receptor recovery.

scholarly journals Niclosamide Triggers Non-Canonical LC3 Lipidation

Cells ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 248 ◽  
Author(s):  
Yajun Liu ◽  
Xia Luo ◽  
Hao Shan ◽  
Yuanyuan Fu ◽  
Qianqian Gu ◽  
...  
Keyword(s):  
Bafilomycin A1 ◽  
Catabolic Pathway ◽  
Pharmacological Activities ◽  
Atg Proteins ◽  
Concanamycin A ◽  
Evolutionarily Conserved ◽  
Atpase Inhibitors ◽  
Cellular Stresses ◽  
Mtor Complex 1 ◽  
Working Mechanisms

Autophagy is a highly- evolutionarily-conserved catabolic pathway activated by various cellular stresses. Recently, non-canonical autophagy (NCA), which does not require all of the ATG proteins to form autophagosome or autophagosome-like structures, has been found in various conditions. Moreover, mounting evidence has indicated that non-canonical LC3 lipidation (NCLL) may reflect NCA. We and others have reported that niclosamide (Nic), an anti-helminthic drug approved by the Food and Drug Administration, could induce canonical autophagy via a feedback downregulation of mTOR complex 1. In this study, we found that Nic could also induce NCLL, which is independent of the ULK1 complex and Beclin 1 complex, but dependent on ubiquitin-like conjugation systems. Although bafilomycin A1 and concanamycin A, two known V-ATPase inhibitors, significantly inhibited Nic-induced NCLL, Nic-induced NCLL was demonstrated to be independent of V-ATPase. In addition, the Golgi complex and vimentin were involved in Nic-induced NCLL, which might be a platform or membrane source for Nic-induced LC3-positive structures. These results would be helpful to broaden our understanding of the working mechanisms of Nic and evaluate its pharmacological activities in diseases.

scholarly journals Increased production of reactive oxygen species by the vacuolar-type (H+)-ATPase inhibitors bafilomycin A1 and concanamycin A in RAW 264 cells

2012 ◽  
Vol 37 (5) ◽  
pp. 1045-1048 ◽  
Author(s):  
Aya Yokomakura ◽  
JangJa Hong ◽  
Kazuo Ohuchi ◽  
Seong-Eun Oh ◽  
Jin-Yong Lee ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Bafilomycin A1 ◽  
Concanamycin A ◽  
Atpase Inhibitors

V-type ATPase is involved in biogenesis of GLUT4 vesicles

2004 ◽  
Vol 287 (3) ◽  
pp. E547-E552 ◽  
Author(s):  
Marina Malikova ◽  
Jun Shi ◽  
Konstantin V. Kandror
Keyword(s):  
Protein Trafficking ◽  
Proton Pumps ◽  
Bafilomycin A1 ◽  
Recycling Endosomes ◽  
Intracellular Membranes ◽  
Concanamycin A ◽  
Endocytic Protein ◽  
Insulin Dependent ◽  
Adipose Cells ◽  
Specific Inhibitors

Proton pumps participate in several aspects of endocytic protein trafficking. However, their involvement specifically in the GLUT4 pathway has been a matter of great controversy. Here, we report that incubation of 3T3-L1 adipocytes with specific inhibitors of V-type ATPase, concanamycin A and bafilomycin A1, inhibits insulin-regulated glucose transport and results in accumulation of GLUT4 in heavy, rapidly sedimenting intracellular membranes. Correspondingly, the amount of small responsive GLUT4 vesicles in concanamycin A- and bafilomycin A1-treated cells is decreased. We conclude that these drugs block translocation of GLUT4 in adipose cells by inhibiting formation of small insulin-responsive vesicles on donor intracellular membranes. At the same time, proton pump inhibitors do not affect insulin-dependent translocation of preexisting vesicles or GLUT4 sorting in recycling endosomes. On the contrary, wortmannin acutely inhibits insulin-dependent translocation of the preexisting vesicles but has no effect on vesicle formation.

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