Novel N-Terminal Sequence of a Glycine-rich Protein in the Aleurone Layer of Soybean Seeds

1994 ◽  
Vol 58 (10) ◽  
pp. 1920-1922 ◽  
Author(s):  
Miyoko Matsui ◽  
Isao Toyosawa ◽  
Mitsuru Fukuda
Keyword(s):  
Soybean Seeds ◽  
Aleurone Layer ◽  
Terminal Sequence ◽  
Glycine Rich Protein

scholarly journals The Structure of the Aleurone Layer and Its Glycine-rich Protein Content in Different Bean Seeds.

1995 ◽  
Vol 48 (6) ◽  
pp. 477-483
Author(s):  
Miyoko MATSUI ◽  
Rie NAKAI ◽  
Isao TOYOSAWA ◽  
Mitsuru FUKUDA
Keyword(s):  
Protein Content ◽  
Aleurone Layer ◽  
Glycine Rich Protein

Isolation of Human Fibrinogen and its Derivatives by Affinity Chromatography on Gly-Pro-Arg-Pro-Lys-Fractogel

1990 ◽  
Vol 63 (03) ◽  
pp. 439-444 ◽  
Author(s):  
C Kuyas ◽  
A Haeberli ◽  
P Walder ◽  
P W Straub
Keyword(s):  
Gel Electrophoresis ◽  
Affinity Purification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Ease Of Use ◽  
Terminal Sequence ◽  
Purification Method ◽  
Human Fibrinogen ◽  
Isolation Methods ◽  
One Step ◽  
Complementary Binding

SummaryWith an immobilized synthetic pentapeptide GlyProArgProLys comprising the N-terminal sequence GlyProArg of the α-chain of fibrin, a new affinity method for the quantitative isolation of fibrinogen out of anticoagulated plasma was developed. The method proved to be superior to all known isolation methods in respect to ease of use and yield, since fibrinogen could be isolated in one step out of plasma with a recovery of more than 95% when compared to the immunologically measurable amounts of fibrinogen. Moreover the amounts of contaminating proteins such as fibronectin, factor XIII or plasminogen were negligible and the purity of the isolated fibrinogen was higher than 95% as measured by polyacrylamide gel electrophoresis. The clottability was 90% and more. Another advantage of this affinity purification method is the possibility to isolate fibrinogen quantitatively out of small plasma samples (<5 ml). Further, abnormal fibrinogen molecules, provided their complementary binding site for GlyProArg is preserved, may also be quantitatively isolated independent of any solubility differences as compared to normal fibrinogen. In addition fibrin(ogcn) fragments originating from plasmic digestion can be separated on the basis of their affinity to GlyProArg. The described affinity gel can be used more than 50 times without any loss of capacity.

Productivity and quality of soybean seeds with different fertilization on podzolized chernozem

2020 ◽  
Vol 1 (97) ◽  
pp. 136-144
Author(s):  
H.M. Hospodarenko ◽  
◽  
I.V. Prokopchuk ◽  
K. P. Leonova ◽  
V.P. Boyko
Keyword(s):  
Crop Rotation ◽  
Crop Yields ◽  
Spring Barley ◽  
Mineral Fertilizer ◽  
Nitrogen Fertilizers ◽  
Mineral Fertilizers ◽  
Forest Steppe ◽  
Soybean Seeds ◽  
Different Doses

The productivity of agricultural crops is the most variable and integral indicator of their vital activity, which accumulates their genetic potential, soil fertility, weather conditions and components of agricultural technology. Soybean under optimal growing conditions (the reaction of the soil is close to neutral, sufficient phosphorus and potassium nutrition, the use of nitraginization) assimilates from the air about 70 % of the total nitrogen requirement. Therefore, it is believed that it is enough to apply only a starting dose of nitrogen fertilizers (20–40 kg/ha a. s.), to get a high yield with good indicators of grain quality. The results of studies of the influence of long-term (8 years) application of different doses and ratios of fertilizers in field crop rotation on podzolized chernozem in the conditions of the Right -Bank Forest-Steppe of Ukraine on the yield and quality of soybean seeds preceded by spring barley were presented. It was found that crop yields could be increased by 18–77 % owing to different doses, ratios and types of fertilizers. The highest indicators of seed yields for three years of the research (3,02 t/ha) were obtained under the application of mineral fertilizers at a dose of N110P60K80 per 1 ha of crop rotation area, including under soybean – N60P60K60. Exclusion of the nitrogen component from the complete fertilizer (N60P60K60) reduced its yield by 26 %, phosphorus – by 17, and potassium by 11 %. There was no significant decrease in soybean yield in the variant of the experiment with a decrease in the proportion of potassium in the composition of complete mineral fertilizer (N60P60K30) for three years of study. The largest mass of 1000 soybean seeds was formed at doses of N60К60 fertilizers, and their protein content — under the application of complete mineral fertilizer in doses of N60P60K60 and N60P60K30.

Antisense RNA-mediated GmFAD2-1B Gene Silencing Enhances Accumulation of Oleic Acid in Transgenic Soybean Seeds

2017 ◽  
Vol 43 (11) ◽  
pp. 1588 ◽  
Author(s):  
Jing YANG ◽  
Guo-Jie XING ◽  
Lu NIU ◽  
Hong-Li HE ◽  
Qian DU ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Gene Silencing ◽  
Antisense Rna ◽  
Soybean Seeds ◽  
Transgenic Soybean

Respiration of Soybean Seeds as Related to Weathering Losses during Ripening 1

1959 ◽  
Vol 51 (11) ◽  
pp. 677-679 ◽  
Author(s):  
Robert W. Howell ◽  
F. I. Collins ◽  
V. E. Sedgwick
Keyword(s):  
Soybean Seeds

Variability in Metabolism of Individual Axes of Soybean Seeds and Its Relationship to Vigor 1

Crop Science ◽  
1975 ◽  
Vol 15 (3) ◽  
pp. 424-426 ◽  
Author(s):  
Robert W. Yaklich ◽  
Aref A. Abdul‐Baki
Keyword(s):  
Soybean Seeds
Sign in / Sign up

Export Citation Format

Share Document