Effects of Plant Growth Regulators and Carbon Sources on Theanine Formation in Callus Cultures of Tea (Camellia sinensis)

1994 ◽  
Vol 58 (8) ◽  
pp. 1519-1521 ◽  
Author(s):  
Takanobu Takihara-Matsuura ◽  
Iwao Sakane ◽  
Takami Kakuda ◽  
Takuya Kitada ◽  
Tatsuyuki Kinoshita ◽  
...  
Planta Medica ◽  
1982 ◽  
Vol 46 (09) ◽  
pp. 15-18 ◽  
Author(s):  
Th. Mulder-Krieger ◽  
R. Verpoorte ◽  
Y. de Graaf ◽  
M. van der Kreek ◽  
A. Baerheim-Svendsen

2009 ◽  
Vol 64 (3-4) ◽  
pp. 239-243 ◽  
Author(s):  
Latiporn Udomsuk ◽  
Kanokwan Jarukamjorn ◽  
Hiroyuki Tanaka ◽  
Waraporn Putalun

Pueraria candollei Wall. ex Benth. var. mirifica (Airy Shaw & Suvat.) Niyomdham was investigated for callus induction using Murashige and Skoog (MS) medium containing different plant growth regulators. After 8 weeks of culture, 66 - 100% of leaf or stem explants formed calli. Calli from stem explants cultured on MS medium supplemented with 0.5 mg/l thidiazuron (TDZ) gave the maximum of shoot induction (16%) and the highest level of total isoflavonoids [(50.39 ± 7.06) mg/g dry wt], which was 7-fold higher than that of the native tuber [(7.04 ± 0.29) mg/g dry wt]. These results suggest that addition of TDZ to the culture medium markedly enhances the production of isoflavonoids in calli induced from stem explants of P. candollei var. mirifica.


Plant Science ◽  
2012 ◽  
Vol 185-186 ◽  
pp. 169-175 ◽  
Author(s):  
Paula Alayón-Luaces ◽  
Nora M.A. Ponce ◽  
Luis A. Mroginski ◽  
Carlos A. Stortz ◽  
Gabriel O. Sozzi

Horticulturae ◽  
2021 ◽  
Vol 7 (11) ◽  
pp. 486
Author(s):  
María Eugenia Martínez ◽  
Lorena Jorquera ◽  
Paola Poirrier ◽  
Katy Díaz ◽  
Rolando Chamy

There are several studies on the medicinal properties of dandelions (Taraxacum officinale), but few studies are aimed at understanding the in vitro germination process of this plant to improve its propagation. This research was focused on studying in vitro seed germination and development of seedlings under different carbon sources, glucose (GLU) or sucrose (SUC) and its concentrations (1.0–5.5%). Additionally, the effect of supplementation with plant growth regulators (PGRs) was studied, measuring the germination capacity, uncertainty and synchrony. Germination was promoted under low carbon source concentrations (≤2.3%), whereas higher concentrations (≥3.2%) had a detrimental effect on this process. GLU allowed the final germination percentages to be slightly better than SUC. Uniformity and synchrony values improved with the presence of PGRs. Results suggested that the best condition to assess T. officinale seed germination is in a medium containing GLU 2.3%, 0.5 mg/L 1-Naphthaleneacetic acid (NAA) and 0.5 mg/L 6-Benzylaminopurine acid (BAP). After germination, the best condition for optimal growth of T. officinale seedlings was 1.0% SUC supplemented with 0.225 mg/L of NAA and 3.0 mg/L of BAP for initial shoot development. The survival rate was 97% after greenhouse acclimatization. This new method of germination was implemented for the massive propagation of T. officinale for further medicinal studies.


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