Purification and Characterization of an Adipamidase from a Mutant Strain ofBrevibacteriumsp. Involved in Dinitrile Degradation

1993 ◽  
Vol 57 (2) ◽  
pp. 294-296 ◽  
Author(s):  
Jean Louis Moreau ◽  
Saïd Azza ◽  
Alain Arnaud ◽  
Pierre Galzy
Keyword(s):  
Mutant Strain ◽  
Purification And Characterization

Purification and characterization of 4-hydroxybenzoate 3-hydroxylase from a Klebsiella pneumoniae mutant strain

1995 ◽  
Vol 164 (1) ◽  
pp. 70-77
Author(s):  
Mónica Suárez ◽  
M. Martín ◽  
Estrella Ferrer ◽  
Amando Garrido-Pertierra
Keyword(s):  
Klebsiella Pneumoniae ◽  
Mutant Strain ◽  
Purification And Characterization

Purification and characterization of 4-hydroxybenzoate 3-hydroxylase from aKlebsiella pneumoniae mutant strain

1995 ◽  
Vol 164 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Mónica Suárez ◽  
Margarita Martín ◽  
Estrella Ferrer ◽  
Amando Garrido-Pertierra
Keyword(s):  
Mutant Strain ◽  
Purification And Characterization

scholarly journals Purification of the Formate-Tetrahydrofolate Ligasefrom Methylobacterium extorquens AM1 and Demonstrationof Its Requirement for MethylotrophicGrowth

2003 ◽  
Vol 185 (24) ◽  
pp. 7169-7175 ◽  
Author(s):  
Christopher J. Marx ◽  
Markus Laukel ◽  
Julia A. Vorholt ◽  
Mary E. Lidstrom
Keyword(s):  
Mutant Strain ◽  
Gene Products ◽  
Wild Type ◽  
Methylobacterium Extorquens ◽  
Type Conversion ◽  
Purification And Characterization ◽  
Formaldehyde Oxidation ◽  
Genes Encoding

ABSTRACT The serine cycle methylotroph Methylobacterium extorquens AM1 contains two pterin-dependent pathways for C1 transfers, the tetrahydrofolate (H4F) pathway and the tetrahydromethanopterin (H4MPT) pathway, and both are required for growth on C1 compounds. With the exception of formate-tetrahydrofolate ligase (FtfL, alternatively termed formyl-H4F synthetase), all of the genes encoding the enzymes comprising these two pathways have been identified, and the corresponding gene products have been purified and characterized. We present here the purification and characterization of FtfL from M. extorquens AM1 and the confirmation that this enzyme is encoded by an ftfL homolog identified previously through transposon mutagenesis. Phenotypic analyses of the ftfL mutant strain demonstrated that FtfL activity is required for growth on C1 compounds. Unlike mutants defective for the H4MPT pathway, the ftfL mutant strain does not exhibit phenotypes indicative of defective formaldehyde oxidation. Furthermore, the ftfL mutant strain remained competent for wild-type conversion of [14C]methanol to [14C]CO2. Collectively, these data confirm our previous presumptions that the H4F pathway is not the key formaldehyde oxidation pathway in M. extorquens AM1. Rather, our data suggest an alternative model for the role of the H4F pathway in this organism in which it functions to convert formate to methylene H4F for assimilatory metabolism.

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