Antifeedant and Insecticidal Activity of Quassinoids against Diamondback Moth (Plutella xylostella)

1993 ◽  
Vol 57 (2) ◽  
pp. 244-246 ◽  
Author(s):  
Masayuki Daido ◽  
Narihiko Fukamiya ◽  
Masayoshi Okano ◽  
Kiyoshi Taoahara ◽  
Makoto Hatakoshi ◽  
...  
Keyword(s):  
Insecticidal Activity ◽  
Plutella Xylostella ◽  
Diamondback Moth

scholarly journals Identification of a native Bacillus thuringiensis strain from Sri Lanka active against Dipel-resistant Plutella xylostella

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7535
Author(s):  
Rashini Yasara Baragamaarachchi ◽  
Jayanetti Koralage Ramani Radhika Samarasekera ◽  
Ovitigala Vithanage Don Sisira Jagathpriya Weerasena ◽  
Kurt Lamour ◽  
Juan Luis Jurat-Fuentes
Keyword(s):  
Bacillus Thuringiensis ◽  
Sri Lanka ◽  
Insecticidal Activity ◽  
Plutella Xylostella ◽  
Multilocus Sequence Typing ◽  
Diamondback Moth ◽  
Sustainable Use ◽  
Field Resistance ◽  
Protein Crystal ◽  
Isolation And Characterization

Background Biopesticides based on strains of the bacterium Bacillus thuringiensis (Bt) are used globally for effective and environmentally friendly pest control. The most serious threat to the sustainable use of these microbial pesticides is the development of resistance on targeted pests. Populations of Plutella xylostella (diamondback moth) have evolved field resistance to Bt pesticides at diverse locations worldwide. Discovery of novel Bt strains with varied toxin profiles that overcome resistance is one of the strategies to increase sustainability of Bt pesticides against P. xylostella. In this study, we report isolation and characterization of a Bt strain named AB1 from Sri Lanka displaying toxicity towards larvae of P. xylostella resistant to the commercial Bt pesticide Dipel. Methods Strains of Bt from diverse environments in Sri Lanka were evaluated for protein crystal production through Differential Interference Contrast (DIC) microscopic examination, and for insecticidal activity against P. xylostella in bioassays. The genome of the AB1 strain was sequenced by Hiseq Illumina sequencing to identify the insecticidal genes present in the genome and nano liquid chromatography followed by tandem mass spectrometry (nanoLC/MS/MS) of purified crystal proteins of AB1 was performed to identify the expressed insecticidal proteins. Multilocus sequence typing and Gyrase B gene sequence analyses were performed to identify the phylogenetic origin of the AB1 strain. Results The AB1 strain was identified as producing high levels of bipyramidal crystals and displaying insecticidal activity against susceptible and Dipel-resistant strains of P. xylostella. Multilocus sequence typing and phylogenetic analysis of the Gyrase B gene identified that AB1 belongs to the B. thuringiensis subsp. aizawai serotype. Comparative analysis of genomic and proteomic data showed that among the insecticidal protein coding genes annotated from the AB1 genome (cry1Aa, cry1Ca, cry1Da, cry1Ia, cry2Ab and cry9), Cry1Ca and Cry1Da toxins represented most of the toxin fraction in parasporal crystals from AB1. Overall findings warrant further development of B. thuringiensis subsp. aizawai AB1 strain as a pesticide to control P. xylostella.

Antifeedant and Insecticidal Activity of Quassinoids against the Diamondback Moth(Plutella xylostella)and Structure—Activity Relationships

1995 ◽  
Vol 59 (6) ◽  
pp. 974-979 ◽  
Author(s):  
Masayuki Daido ◽  
Nobuhiro Ohno ◽  
Kazunori Imamura ◽  
Narihiko Fukamiya ◽  
Makoto Hatakoshi ◽  
...  
Keyword(s):  
Insecticidal Activity ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Structure Activity Relationships ◽  
Structure Activity

scholarly journals AKTIVITAS INSEKTISIDA EKSTRAK TITHONIA DIVERSIFOLIA (HEMSL.) A GRAY (ASTERACEAE) TERHADAP ULAT DAUN KUBIS PLUTELLA XYLOSTELLA (L.) (LEPIDOPTERA: YPONOMEUTIDAE)

2017 ◽  
Vol 17 (2) ◽  
pp. 185
Author(s):  
Efrin Firmansyah ◽  
Dadang . ◽  
Ruli Anwar
Keyword(s):  
Topical Application ◽  
Insecticidal Activity ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Probit Analysis ◽  
Botanical Insecticides ◽  
Tithonia Diversifolia ◽  
Mortality Effect ◽  
Insect Mortality ◽  
Cruciferous Plants

Insecticidal activity of extracts of Tithonia diversifolia (Hemsl.) A. Gray (Asteraceae) against the diamondback moth Plutella xylostella (L.) (Lepidoptera: Yponomeutidae). The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Yponomeutidae) is one of the most destructive pests on cruciferous plants. Generally farmers use synthetic insecticides to control this pest. Intensive and excessive use of insecticides can cause some undesirable effects such as resistance, resurgence, and contamination of insecticide on environment. One effort to solve the problems caused by synthetic insecticides use is by utilization of botanical materials that are potential to be used as botanical insecticides, one of them is Tithonia diversifolia (Asteraceae). The purpose of this research was to study the insecticidal activity of T. diversifolia as a botanical insecticide against P. xylostella. The leaf residual and topical application methods were used to assess the mortality effect of the extracts. Insect mortality rate was analyzed using probit analysis to obtain LC50 and LC95 values. The results showed that the flower extract had better activity on insect mortality than the leaf extract both in leaf residual and topical application treatments.

Insecticidal and synergistic activity of two monoterpenes against diamondback moth (Lepidoptera: Plutellidae)

2018 ◽  
Vol 150 (2) ◽  
pp. 258-264 ◽  
Author(s):  
A.E. Webster ◽  
P. Manning ◽  
J.M. Sproule ◽  
N. Faraone ◽  
G.C. Cutler
Keyword(s):  
Insecticidal Activity ◽  
Plutella Xylostella ◽  
Diamondback Moth ◽  
Oral Exposure ◽  
Synergistic Activity ◽  
Low Concentrations

AbstractWe examined the insecticidal activity of linalool and thymol, against diamondback moth,Plutella xylostella(Linnaeus) (Lepidoptera: Plutellidae), and whether they would synergise the activity of spinosad against this pest. Both linalool and thymol were toxic to diamondback moth larvae by topical and oral exposure, but orders of magnitude less so than spinosad. We found that low concentrations of linalool weakly synergised spinosad, increasing its toxicity more than twofold. An interaction between spinosad, and acetone meant it was not possible to identify any potential synergisms between thymol and spinosad. Our results demonstrate limited potential for thymol and linalool to act as biopesticides or synergists for managing diamondback moth.

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