Overproduction of Hyperthermostable β-1,4-Endoglucanase from the ArchaeonPyrococcus horikoshiiby Tobacco Chloroplast Engineering

2013 ◽  
Vol 77 (10) ◽  
pp. 2140-2143 ◽  
Author(s):  
Yoichi NAKAHIRA ◽  
Kazuhiko ISHIKAWA ◽  
Kunisuke TANAKA ◽  
Yuzuru TOZAWA ◽  
Takashi SHIINA
Keyword(s):  
Tobacco Chloroplast

scholarly journals An additional promoter within the protein-coding region of thepsbD-psbC gene cluster in tobacco chloroplast DNA

1989 ◽  
Vol 17 (23) ◽  
pp. 9583-9591 ◽  
Author(s):  
Wen Biao Yao ◽  
Bing Yuan Meng ◽  
Minoru Tanaka ◽  
Masahiro Sugiura
Keyword(s):  
Chloroplast Dna ◽  
Gene Cluster ◽  
Coding Region ◽  
Protein Coding ◽  
Tobacco Chloroplast

Import and processing of the precursor of the delta subunit of tobacco chloroplast ATP synthase

1992 ◽  
Vol 20 (3) ◽  
pp. 549-554 ◽  
Author(s):  
J. A. Napier ◽  
K. H. Larsson ◽  
F. Madue�o ◽  
J. C. Gray
Keyword(s):  
Atp Synthase ◽  
Chloroplast Atp Synthase ◽  
Tobacco Chloroplast ◽  
Delta Subunit

scholarly journals Cloning and Nucleotide Sequence of a Tobacco Chloroplast Translational Elongation Factor, EF-Tu

1993 ◽  
Vol 101 (1) ◽  
pp. 333-334 ◽  
Author(s):  
V. M. Ursin ◽  
C. K. Becker ◽  
C. K. Shewmaker
Keyword(s):  
Nucleotide Sequence ◽  
Elongation Factor ◽  
Tobacco Chloroplast

The gene for the 9 kd polypeptide, a possible apoprotein for the iron-sulfur centers A and B of the photosystem I complex, in tobacco chloroplast DNA

1987 ◽  
Vol 12 (4) ◽  
pp. 247-250 ◽  
Author(s):  
Nobuaki Hayashidal ◽  
Tohru Matsubayashi ◽  
Kazuo Shinozaki ◽  
Masahiro Sugiura ◽  
Keisuke Inoue ◽  
...  
Keyword(s):  
Chloroplast Dna ◽  
Photosystem I ◽  
Tobacco Chloroplast ◽  
Iron Sulfur ◽  
Photosystem I Complex

scholarly journals A novel protein with DNA binding activity from tobacco chloroplast nucleoids.

1997 ◽  
Vol 9 (9) ◽  
pp. 1673-1682 ◽  
Author(s):  
T Nakano ◽  
S Murakami ◽  
T Shoji ◽  
S Yoshida ◽  
Y Yamada ◽  
...  
Keyword(s):  
Dna Binding ◽  
Binding Activity ◽  
Tobacco Chloroplast ◽  
Dna Binding Activity ◽  
Chloroplast Nucleoids ◽  
Novel Protein

scholarly journals Complementation of the yeast deletion mutant DeltaNCE103 by members of the beta class of carbonic anhydrases is dependent on carbonic anhydrase activity rather than on antioxidant activity

2004 ◽  
Vol 379 (3) ◽  
pp. 609-615 ◽  
Author(s):  
Daniel CLARK ◽  
Roger S. ROWLETT ◽  
John R. COLEMAN ◽  
Daniel F. KLESSIG
Keyword(s):  
Antioxidant Activity ◽  
Type Strain ◽  
Deletion Mutant ◽  
Wild Type Strain ◽  
Carbonic Anhydrase Activity ◽  
Site Directed Mutagenesis ◽  
Carbonic Anhydrases ◽  
Wild Type ◽  
Tobacco Chloroplast ◽  
Low Levels

In recent years, members of the β class of CAs (carbonic anhydrases) have been shown to complement ΔNCE103, a yeast strain unable to grow under aerobic conditions. The activity required for complementation of ΔNCE103 by tobacco chloroplast CA was studied by site-directed mutagenesis. E196A (Glu196→Ala), a mutated tobacco CA with low levels of CA activity, complemented ΔNCE103. To determine whether restoration of ΔNCE103 was due to residual levels of CA activity or whether it was related to previously proposed antioxidant activity of CAs [Götz, Gnann and Zimmermann (1999) Yeast 15, 855–864], additional complementation analysis was performed using human CAII, an α CA structurally unrelated to the β class of CAs to which the tobacco protein belongs. Human CAII complemented ΔNCE103, strongly arguing that CA activity is responsible for the complementation of ΔNCE103. Consistent with this conclusion, recombinant NCE103 synthesized in Escherichia coli shows CA activity, and ΔNCE103 expressing the tobacco chloroplast CA exhibits the same sensitivity to H2O2 as the wild-type strain.

An atpE-specific promoter within the coding region of the atpB gene in tobacco chloroplast DNA

1994 ◽  
Vol 26 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Sanjay Kapoor ◽  
Tatsuya Wakasugi ◽  
Hiroshi Deno ◽  
Masahiro Sugiura
Keyword(s):  
Chloroplast Dna ◽  
Coding Region ◽  
Tobacco Chloroplast
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