Protective Effect of DetoxifiedRhus vernicifluaStokes on Human Keratinocytes and Dermal Fibroblasts against Oxidative Stress and Identification of the Bioactive Phenolics

2013 ◽  
Vol 77 (8) ◽  
pp. 1682-1688 ◽  
Author(s):  
Chun-Shan LIU ◽  
Tae-Gyu NAM ◽  
Min-Woo HAN ◽  
Soo-mi AHN ◽  
Han Seok CHOI ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Human Keratinocytes ◽  
Dermal Fibroblasts

Protective effect of polysaccharides from Sargassum fusiforme against UVB-induced oxidative stress in HaCaT human keratinocytes

2017 ◽  
Vol 36 ◽  
pp. 332-340 ◽  
Author(s):  
Desheng Ji ◽  
Lijun You ◽  
Yinglang Ren ◽  
Lingrong Wen ◽  
Guiqing Zheng ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Human Keratinocytes ◽  
Sargassum Fusiforme

Protective effect of indigo naturalis extract against oxidative stress in cultured human keratinocytes

2012 ◽  
Vol 139 (3) ◽  
pp. 893-896 ◽  
Author(s):  
Yin-Ku Lin ◽  
Hsiao-Wen Chen ◽  
Sien-Hung Yang ◽  
Yann-Lii Leu ◽  
Yu-Hsiang Huang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Human Keratinocytes ◽  
Indigo Naturalis

UVB induced oxidative stress in human keratinocytes and protective effect of antioxidant agents

2007 ◽  
Vol 46 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Guang-Hui Jin ◽  
Yang Liu ◽  
Shun-Zi Jin ◽  
Xiao-Dong Liu ◽  
Shu-Zheng Liu
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Human Keratinocytes ◽  
Antioxidant Agents

Protective effect of Opuntia ficus - indica L. cladodes against UVA-induced oxidative stress in normal human keratinocytes

2017 ◽  
Vol 27 (24) ◽  
pp. 5485-5489 ◽  
Author(s):  
Ganna Petruk ◽  
Flaviana Di Lorenzo ◽  
Paola Imbimbo ◽  
Alba Silipo ◽  
Andrea Bonina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Human Keratinocytes ◽  
Opuntia Ficus Indica ◽  
Normal Human Keratinocytes ◽  
Normal Human

scholarly journals Protective Effect of Merbau (Intsia bijuga) Extract on Hydrogen Peroxide-Treated HaCaT Human Keratinocytes and Its Formulation as Antioxidant Cream

2020 ◽  
Vol 27 (1) ◽  
pp. 131-138
Author(s):  
Leonny Yulita Hartiadi ◽  
Agnes Anania Triavika sahamastuti ◽  
Cynthia Valentina Chandra ◽  
Erlina Febriani ◽  
Shakila Angyaja Adiyanto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Human Keratinocytes ◽  
Skin Aging ◽  
Ph Values ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
Dried Extract ◽  
Evaluation Parameters

Background: The excessive generation of ROS in the skin results in oxidative stress that can contribute to premature skin aging, inflammation, and skin carcinogenesis. To prevent these detrimental effects, the development of herbal medicine with a potent antioxidant activity into cosmetic products is required. This study aims to formulate cream that contains a safe and effective concentration of merbau (Intsia bijuga), which has been shown to have a strong antioxidant activity. Methods: Powdered merbau wood was macerated with methanol and the dried extract was evaluated for its cytotoxic effect and antioxidant activity on human keratinocytes cell line using MTS assay. Five cream formulations containing the extract were made and subjected to stability and physical evaluations, including organoleptic, types of cream, pH, viscosity, and homogeneity. Results: Cytotoxicity assay revealed that merbau extracts had an IC50 of 181.3 μg/mL (95% confidence interval (CI): 165.4 - 200.1 µg/mL). At a concentration of 31.25 μg/mL, the extract exhibited a protective effect against H2O2-induced oxidative stress, comparable to vitamin E. Five cream formulas that were developed demonstrated good physical properties that fulfilled the evaluation parameters, including o/w type of cream, homogenous, and stable based on centrifugation and freeze-thaw cycle tests. The pH values were between 5.65 ± 0.067 - 7.4 ± 0.050, while the viscosity values were between 131 ± 1.249 - 56,011 ± 2,729.27 mPa.s. All cream formulas exhibited shear-thinning properties upon increasing shear stress. Conclusion: Overall, this study has successfully formulated several cream formulations containing merbau extract at a concentration that shows antioxidant activity.

scholarly journals Oat (Avena sativa) Extract against Oxidative Stress-Induced Apoptosis in Human Keratinocytes

Molecules ◽  
2021 ◽  
Vol 26 (18) ◽  
pp. 5564
Author(s):  
Sooji Song ◽  
Yoon-Mi Lee ◽  
Yu Young Lee ◽  
Kyung-Jin Yeum
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Cell Death ◽  
Protective Effect ◽  
Avena Sativa ◽  
Ethanol Extract ◽  
Human Keratinocytes ◽  
Hacat Cells ◽  
Ethanol Extracts ◽  
Induced Apoptosis

Oat (Avena sativa) is well known for its various health benefits. The protective effect of oat extract against oxidative stress-induced apoptosis in human keratinocytes HaCaT was determined. First, extracts of two varieties of oat, Daeyang and Choyang, were analyzed for fat-soluble antioxidants such as α-tocotrienol, γ-oryzanols, lutein and zeaxanthin using an UPLC system and for antioxidant activity using a DPPH assay. Specifically, an 80% ethanol extract of Daeyang oat (Avena sativa cv. Daeyang), which had high amounts of antioxidants and potent radical scavenging activity, was further evaluated for protective effect against oxidative stress-induced cell death, intracellular reactive oxygen species levels, the phosphorylation of DNA damage mediating genes such as H2AX, checkpoint kinase 1 and 2, and p53 and the activation of apoptotic genes such as cleaved caspase-3 and 7 and poly (ADP-ribose) polymerase in HaCaT cells. The Daeyang and Choyang oat 80% ethanol extracts had 26.9 and 24.1 mg/100 g γ-oryzanols, 7.69 and 8.38 mg/100 g α-tocotrienol, 1.25 and 0.34 mg/100 g of lutein and 1.20 and 0.17 mg/100 g of zeaxanthin, respectively. The oat 80% ethanol extract treatment (Avena sativa cv. Daeyang) had a protective effect on oxidative stress-induced cell death in HaCaT cells. In addition, the oat 80% ethanol extracts led to a significant decrease in the intracellular ROS level at a concentration of 50–200 μg/mL, the attenuation of DNA damage mediating genes and the inhibition of apoptotic caspase activities in a dose dependent manner (50–200 μg/mL). Thus, the current study indicates that an oat (Avena sativa cv. Daeyang) extract rich in antioxidants, such as polyphenols, avenanthramides, γ-oryzanols, tocotrienols and carotenoids, has a protective role against oxidative stress-induced keratinocyte injuries and that oat may a useful source for oxidative stress-associated skin damage.

scholarly journals The Protective Effect of Carotenoids, Polyphenols, and Estradiol on Dermal Fibroblasts under Oxidative Stress

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2023
Author(s):  
Aya Darawsha ◽  
Aviram Trachtenberg ◽  
Joseph Levy ◽  
Yoav Sharoni
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Fruits And Vegetables ◽  
Antioxidant Defense System ◽  
Cell Number ◽  
Dermal Fibroblasts ◽  
Hormonal Changes ◽  
Skin Damage ◽  
Collagen Secretion ◽  
Skin Physiology

Skin ageing is influenced by several factors including environmental exposure and hormonal changes. Reactive oxygen species (ROS), which mediate many of the effects of these factors, induce inflammatory processes in the skin and increase the production of matrix metalloproteinases (MMPs) in dermal fibroblasts, which leads to collagen degradation. Several studies have shown the protective role of estrogens and a diet rich in fruits and vegetables on skin physiology. Previous studies have shown that dietary carotenoids and polyphenols activate the cell’s antioxidant defense system by increasing antioxidant response element/Nrf2 (ARE/Nrf2) transcriptional activity and reducing the inflammatory response. The aim of the current study was to examine the protective effect of such dietary-derived compounds and estradiol on dermal fibroblasts under oxidative stress induced by H2O2. Human dermal fibroblasts were used to study the effect of H2O2 on cell number and apoptosis, MMP-1, and pro-collagen secretion as markers of skin damage. Treatment of cells with H2O2 led to cell death, increased secretion of MMP-1, and decreased pro-collagen secretion. Pre-treatment with tomato and rosemary extracts, and with estradiol, reversed the effects of the oxidative stress. This was associated with a reduction in intracellular ROS levels, probably through the measured increased activity of ARE/Nrf2. Conclusions: This study indicates that carotenoids, polyphenols, and estradiol protect dermal fibroblasts from oxidative stress-induced damage through a reduction in ROS levels.

Antioxidant Activity and Protective Effect against Oxidative Stress on Hippocampal HT22 Cells of Tea Seed Oil

2018 ◽  
Vol 24 (1) ◽  
pp. 53-59
Author(s):  
Jong Min Kim ◽  
Seon Kyeong Park ◽  
Jin Yong Kang ◽  
Seong-kyeong Bae ◽  
Ga-Hee Jeong ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Protective Effect ◽  
Seed Oil ◽  
Ht22 Cells ◽  
Tea Seed Oil

Protective effect of DJ-1 against oxidative stress: an advance

2012 ◽  
Vol 32 (1) ◽  
pp. 88-91
Author(s):  
Zhi-yong WANG ◽  
Ling-zhen TANG ◽  
Tian-wen GAO
Keyword(s):  
Oxidative Stress ◽  
Protective Effect
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