The Mutational Effect of Ile58 at Subsite C in Hen Egg-White Lysozyme on Substrate Binding, Enzymatic Activity, and Protein Stability

2013 ◽  
Vol 77 (3) ◽  
pp. 560-565 ◽  
Author(s):  
Shunsuke KAWAMURA ◽  
Yuki CHIJIIWA ◽  
Takao TORIKATA ◽  
Tomohiro ARAKI
Keyword(s):  
Enzymatic Activity ◽  
Protein Stability ◽  
Substrate Binding ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Mutational Effect ◽  
Hen Egg

scholarly journals HEAVY METAL-INDUCED INHIBITION OF ENZYMATIC ACTIVITY IN HEN EGG WHITE LYSOZYME

2018 ◽  
Vol 11 (11) ◽  
pp. 212
Author(s):  
Michel Pazmiño ◽  
Silva Monica ◽  
Welbaum James ◽  
Gaibor Juan ◽  
Altuna Jose Luis ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Enzymatic Activity ◽  
Egg White ◽  
Activity Levels ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

Objective: The objective of this study was to determine the content of chromium (Cr), nickel (Ni), cadmium (Cd), boron (B), mercury (Hg), and lead (Pb) in chicken hen eggs in hen’s eggs and to evaluate their influence on the enzymatic activity of hen egg white lysozyme (HEWL).Methods: In this study, we have determined the content of Cr, Ni, Cd, B, Hg, and Pb in chicken hen eggs using absorption atomic (AA) method. The residual enzymatic activity of HEWL, HEWL was evaluated using spectrophotometric method.Results: The levels of Cr, Ni, Cd, B, Hg, and Pb in hen eggs were 1.8300, 1.1300, 0.5350, 0.5200, 0.0025, and 0.0021 mg/kg, respectively. The inhibition of enzymatic activity of HEWL, by exposure to heavy metals was studied. We found that Cr, Ni, Cd, B, Hg, and Pb produced a loss of enzymatic activity. Cr III was the metal with the highest reduction of HEWL enzymatic activity. 0.01 M of Cr III was able to reduce HEWL enzymatic activity levels up to 49% after 24 h of exposure to this metal. An exposure of 0.05 M of Cr III reduced HEWL enzymatic activity to 1%. 24 h exposure to 0.01 M of Cd presented a reduction of 37% of HEWL enzymatic activity and 0.05 M of Cd resulted in only 75% of HEWL enzymatic residual activity.Conclusions: Content of heavy metals in hen egg from Tungurahua region was high. Cr, Ni, Cd, B, Hg, and Pb produced a loss of enzymatic activity of HEWL.

scholarly journals Effects of subsite alterations on substrate-binding mode in the active site of hen egg-white lysozyme

1993 ◽  
Vol 212 (1) ◽  
pp. 151-156 ◽  
Author(s):  
Izumi KUMAGAI ◽  
Katsumi MAENAKA ◽  
Futoshi SUNADA ◽  
Shigeki TAKEDA ◽  
Kin-ichiro MIURA
Keyword(s):  
Active Site ◽  
Substrate Binding ◽  
Binding Mode ◽  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

Inactivation of hen egg-white lysozyme. The azide radical

1997 ◽  
Vol 94 ◽  
pp. 356-364 ◽  
Author(s):  
M Faraggi ◽  
E Bettelheim ◽  
M Weinstein
Keyword(s):  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Hen Egg White ◽  
Hen Egg

The inhibitory role of clioquinol in the fibrillation of hen egg white lysozyme

2021 ◽  
pp. 138830
Author(s):  
Baoliang Ma ◽  
Haohao Wang ◽  
Yujie Liu ◽  
Fang Wu ◽  
Xudong Zhu
Keyword(s):  
Egg White ◽  
Hen Egg White Lysozyme ◽  
Egg White Lysozyme ◽  
Inhibitory Role ◽  
Hen Egg White ◽  
Hen Egg
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