scholarly journals Visualization of the Mycelia of Wood-Rotting Fungi by Fluorescence in Situ Hybridization Using a Peptide Nucleic Acid Probe

2013 ◽  
Vol 77 (2) ◽  
pp. 405-408 ◽  
Author(s):  
Yuji NAKADA ◽  
Satoshi NAKABA ◽  
Hiroshi MATSUNAGA ◽  
Ryo FUNADA ◽  
Makoto YOSHIDA
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Peptide Nucleic Acid Probe

scholarly journals Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Cronobacter Genomospecies (Enterobacter sakazakii) in Powdered Infant Formula

2009 ◽  
Vol 75 (9) ◽  
pp. 2925-2930 ◽  
Author(s):  
C. Almeida ◽  
N. F. Azevedo ◽  
C. Iversen ◽  
S. Fanning ◽  
C. W. Keevil ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Infant Formula ◽  
Peptide Nucleic Acid ◽  
Powdered Infant Formula ◽  
Enterobacter Sakazakii ◽  
Specific Detection ◽  
Peptide Nucleic Acid Probe ◽  
Bacterial Contaminants ◽  
Specificity And Sensitivity

ABSTRACT Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.

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