scholarly journals Transcriptional Control of the Isoeugenol Monooxygenase ofPseudomonas nitroreducensJin1 inEscherichia coli

2012 ◽  
Vol 76 (10) ◽  
pp. 1891-1896 ◽  
Author(s):  
Ji-Young RYU ◽  
Jiyoung SEO ◽  
Joong-Hoon AHN ◽  
Michael J. SADOWSKY ◽  
Hor-Gil HUR
Keyword(s):  
Transcriptional Control ◽  
Inescherichia Coli ◽  
Isoeugenol Monooxygenase

scholarly journals Post-transcriptional control of Crp-cAMP by RNase LS inEscherichia coli

2008 ◽  
Vol 70 (6) ◽  
pp. 1570-1578 ◽  
Author(s):  
Akira Iwamoto ◽  
Sébastien Lemire ◽  
Tetsuro Yonesaki
Keyword(s):  
Transcriptional Control ◽  
Inescherichia Coli

scholarly journals Regulation of iron-sulphur cluster homeostasis through transcriptional control of the Isc pathway by [2Fe-2S]-IscR inEscherichia coli

2012 ◽  
Vol 87 (3) ◽  
pp. 478-492 ◽  
Author(s):  
Jennifer L. Giel ◽  
April D. Nesbit ◽  
Erin L. Mettert ◽  
Angela S. Fleischhacker ◽  
Brendan T. Wanta ◽  
...  
Keyword(s):  
Transcriptional Control ◽  
Inescherichia Coli

scholarly journals Negative Control of rpoS Expression by Phosphoenolpyruvate:Carbohydrate Phosphotransferase System inEscherichia coli

2001 ◽  
Vol 183 (2) ◽  
pp. 520-527 ◽  
Author(s):  
Chiharu Ueguchi ◽  
Naoko Misonou ◽  
Takeshi Mizuno
Keyword(s):  
Cyclic Amp ◽  
Carbon Metabolism ◽  
Translational Control ◽  
Transcriptional Control ◽  
Molecular Mechanisms ◽  
Negative Control ◽  
Inescherichia Coli ◽  
Phosphotransferase System ◽  
Number Of Factors ◽  
Complex Manner

ABSTRACT The ςS (or ς38) subunit of RNA polymerase, encoded by the rpoS gene, is a crucial regulator in the transcriptional control of a set of genes under stressful conditions, such as nutrient starvation. The expression ofrpoS is regulated in a complex manner at the levels of transcription, translation, and stability of the product. Although a number of factors involved in the regulation of rpoSexpression have been identified, the underlying molecular mechanisms are not fully understood. In this study, we identified the Crr (or EIIAGlc) protein as a novel factor that plays an important role not only in the transcriptional control but also in the translational control of rpoS expression. Crr is an important component in glucose uptake through the well-characterized phosphoenolpyruvate:carbohydrate phosphotransferase system. The results of a series of genetic analyses revealed that Crr negatively controlsrpoS translation and transcription. The observed transcriptional control by Crr appears to be mediated by cyclic AMP. However, it was found that Crr negatively controls rpoStranslation rather directly. These results suggest a possible linkage between the control of rpoS expression and carbon metabolism.

Nutrient-regulated gene expression in eukaryotes

2006 ◽  
Vol 73 ◽  
pp. 85-96 ◽  
Author(s):  
Richard J. Reece ◽  
Laila Beynon ◽  
Stacey Holden ◽  
Amanda D. Hughes ◽  
Karine Rébora ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Transcriptional Control ◽  
Direct Interaction ◽  
Signalling Pathways ◽  
A Cell ◽  
One Step ◽  
Higher Eukaryotes ◽  
Regulated Gene Expression

The recognition of changes in environmental conditions, and the ability to adapt to these changes, is essential for the viability of cells. There are numerous well characterized systems by which the presence or absence of an individual metabolite may be recognized by a cell. However, the recognition of a metabolite is just one step in a process that often results in changes in the expression of whole sets of genes required to respond to that metabolite. In higher eukaryotes, the signalling pathway between metabolite recognition and transcriptional control can be complex. Recent evidence from the relatively simple eukaryote yeast suggests that complex signalling pathways may be circumvented through the direct interaction between individual metabolites and regulators of RNA polymerase II-mediated transcription. Biochemical and structural analyses are beginning to unravel these elegant genetic control elements.

MicroRNAs regulate aldosterone signaling by post-transcriptional control of mineralocorticoid receptor expression

2019 ◽  
Author(s):  
Thi-An Vu ◽  
Ingrid Lema ◽  
Jerome Bouligand ◽  
Laetitia Martinerie ◽  
Marc Lombes ◽  
...  
Keyword(s):  
Mineralocorticoid Receptor ◽  
Transcriptional Control ◽  
Receptor Expression

Post-Transcriptional Regulation of Cardiac Sarcomere Protein Titin Through 3’ Untranslated Regions

2013 ◽  
Vol 9 ◽  
Author(s):  
Tara A Shrout
Keyword(s):  
Gene Expression ◽  
Transcriptional Control ◽  
Striated Muscle ◽  
Binding Capacity ◽  
Structural Features ◽  
Neonatal Rat ◽  
Regulatory Control ◽  
Untranslated Regions ◽  
Luciferase Reporter ◽  
Regulatory Effects

Titin is the largest known protein in the human body, and forms the backbone of all striated muscle sarcomeres. The elastic nature of titin is an important component of muscle compliance and functionality. A significant amount of energy is expended to synthesize titin, thus we postulate that titin gene expression is under strict regulatory control in order to conserve cellular resources. In general, gene expression is mediated in part by post-transcriptional control elements located within the 5’ and 3’ untranslated regions (UTRs) of mature mRNA. The 3’UTR in particular contains structural features that affect binding capacity to other RNA components, such as MicroRNA, which control mRNA localization, translation, and degradation. The degree and significance of the regulatory effects mediated by two determined variants of titin’s 3’ UTR were evaluated in Neonatal Rat Ventricular Myocyte and Human Embryonic Kidney cell lines. Recombinant plasmids to transfect these cells lines were engineered by insertion of the variant titin 3’UTR 431- and 1047-base pairs sequences into luciferase reporter vectors. Expression due to an unaltered reporter vector served as the control. Quantitative changes in luciferase activity due to the recombinants proportionally represented the effect titin’s respective 3’UTR conferred on downstream post-transcriptional expression relative to the control. The effect due to titin’s shorter 3’UTR sequence was inconclusive; however, results illustrated that titin’s longer 3’UTR sequence caused a 35 percent decrease in protein expression. Secondary structural analysis of the two sequences revealed differential folding patterns that affect the stability and degree of MicroRNA-binding within titin’s variant 3’UTR sequences.

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