The JAK2/STAT3 Signal Pathway Regulates the Expression of Genes Related to Skeletal Muscle Development and Energy Metabolism in Mice and Mouse Skeletal Muscle Cells

2012 ◽  
Vol 76 (10) ◽  
pp. 1866-1870 ◽  
Author(s):  
Jing LIU ◽  
Xubin JING ◽  
Lu GAN ◽  
Chao SUN
Keyword(s):  
Skeletal Muscle ◽  
Energy Metabolism ◽  
Muscle Development ◽  
Muscle Cells ◽  
Signal Pathway ◽  
Skeletal Muscle Cells ◽  
Skeletal Muscle Development ◽  
Mouse Skeletal Muscle ◽  
Expression Of Genes

scholarly journals Differentially expressed fibroblast growth factors regulate skeletal muscle development through autocrine and paracrine mechanisms.

1996 ◽  
Vol 132 (6) ◽  
pp. 1151-1159 ◽  
Author(s):  
K Hannon ◽  
A J Kudla ◽  
M J McAvoy ◽  
K L Clase ◽  
B B Olwin
Keyword(s):  
Skeletal Muscle ◽  
Dna Synthesis ◽  
Muscle Cell ◽  
Muscle Development ◽  
Muscle Cells ◽  
Skeletal Muscle Cell ◽  
Skeletal Muscle Cells ◽  
Dominant Negative Mutant ◽  
Skeletal Muscle Development ◽  
Global Effect

Several FGF family members are expressed in skeletal muscle; however, the roles of these factors in skeletal muscle development are unclear. We examined the RNA expression, protein levels, and biological activities of the FGF family in the MM14 mouse skeletal muscle cell line. Proliferating skeletal muscle cells express FGF-1, FGF-2, FGF-6, and FGF-7 mRNA. Differentiated myofibers express FGF-5, FGF-7, and reduced levels of FGF-6 mRNA. FGF-3, FGF-4, and FGF-8 were not detectable by RT-PCR in either proliferating or differentiated skeletal muscle cells. FGF-I and FGF-2 proteins were present in proliferating skeletal muscle cells, but undetectable after terminal differentiation. We show that transfection of expression constructs encoding FGF-1 or FGF-2 mimics the effects of exogenously applied FGFs, inhibiting skeletal muscle cell differentiation and stimulating DNA synthesis. These effects require activation of an FGF tyrosine kinase receptor as they are blocked by transfection of a dominant negative mutant FGF receptor. Transient transfection of cells with FGF-1 or FGF-2 expression constructs exerted a global effect on myoblast DNA synthesis, as greater than 50% of the nontransfected cells responded by initiating DNA synthesis. The global effect of cultures transfected with FGF-2 expression vectors was blocked by an anti-FGF-2 monoclonal antibody, suggesting that FGF-2 was exported from the transfected cells. Despite the fact that both FGF-l and FGF-2 lack secretory signal sequences, when expressed intracellularly, they regulate skeletal muscle development. Thus, production of FGF-1 and FGF-2 by skeletal muscle cells may act as a paracrine and autocrine regulator of skeletal muscle development in vivo.

scholarly journals Loss of caveolin-3 induced by the dystrophy-associated P104L mutation impairs L-type calcium channel function in mouse skeletal muscle cells

2007 ◽  
Vol 580 (3) ◽  
pp. 745-754 ◽  
Author(s):  
Harold Couchoux ◽  
Bruno Allard ◽  
Claude Legrand ◽  
Vincent Jacquemond ◽  
Christine Berthier
Keyword(s):  
Skeletal Muscle ◽  
Calcium Channel ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Mouse Skeletal Muscle ◽  
Channel Function

Energy metabolism profile of the effects of amino acid treatment on skeletal muscle cells: Leucine inhibits glycolysis of myotubes

Nutrition ◽  
2020 ◽  
Vol 77 ◽  
pp. 110794 ◽  
Author(s):  
Reiko Suzuki ◽  
Yoriko Sato ◽  
Kodwo Amuzuah Obeng ◽  
Daisuke Suzuki ◽  
Yusuke Komiya ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Amino Acid ◽  
Energy Metabolism ◽  
Acid Treatment ◽  
Muscle Cells ◽  
Skeletal Muscle Cells
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