Bacterial Surface Display of a Co-Factor Containing Enzyme, ω-Transaminase fromVibrio fluvialisUsing theBacillus subtilisSpore Display System

2011 ◽  
Vol 75 (9) ◽  
pp. 1862-1865 ◽  
Author(s):  
Bum-Yeol HWANG ◽  
Byung-Gee KIM ◽  
June-Hyung KIM
Keyword(s):  
Surface Display ◽  
Display System ◽  
Bacterial Surface ◽  
Bacterial Surface Display

Development of a novel bacterial surface display system using truncated OmpT as an anchoring motif

2019 ◽  
Vol 41 (6-7) ◽  
pp. 763-777 ◽  
Author(s):  
Chang-ye Hui ◽  
Yan Guo ◽  
Lisa Liu ◽  
Hao-qu Zheng ◽  
Hong-min Wu ◽  
...  
Keyword(s):  
Surface Display ◽  
Display System ◽  
Bacterial Surface ◽  
Bacterial Surface Display ◽  
Anchoring Motif

scholarly journals An Effective Platform for Exploring Rotavirus Receptors by Bacterial Surface Display System

2019 ◽  
Vol 35 (1) ◽  
pp. 103-109
Author(s):  
Danlei Liu ◽  
Haoran Geng ◽  
Zilei Zhang ◽  
Yifan Xing ◽  
Danlu Yang ◽  
...  
Keyword(s):  
Surface Display ◽  
Display System ◽  
Bacterial Surface ◽  
Bacterial Surface Display

scholarly journals Chimeric FimH adhesin of type 1 fimbriae: a bacterial surface display system for heterologous sequences

Microbiology ◽  
1995 ◽  
Vol 141 (11) ◽  
pp. 2839-2848 ◽  
Author(s):  
L. Pallesen ◽  
L. K. Poulsen ◽  
G. Christiansen ◽  
P. Klemm
Keyword(s):  
Surface Display ◽  
Display System ◽  
Bacterial Surface ◽  
Type 1 Fimbriae ◽  
Bacterial Surface Display

scholarly journals Ice nucleation protein as a bacterial surface display protein

2011 ◽  
Vol 63 (4) ◽  
pp. 943-948 ◽  
Author(s):  
Mohammed Sarhan
Keyword(s):  
Recombinant Proteins ◽  
Surface Display ◽  
Ice Nucleation ◽  
Display System ◽  
Ice Nucleation Protein ◽  
Bacterial Surface ◽  
Sorting Signals ◽  
Bacterial Surface Display ◽  
Display Technology ◽  
Anchoring Motif

Surface display technology can be defined as that phenotype (protein or peptide) which is linked to a genotype (DNA or RNA) through an appropriate anchoring motif. A bacterial surface display system is based on expressing recombinant proteins fused to sorting signals (anchoring motifs) that direct their incorporation on the cell surface.

scholarly journals Bacterial Surface Display of Levansucrase of Zymomonas mobilis Using Bacillus Subtilis Spore Display System

KSBB Journal ◽  
2011 ◽  
Vol 26 (3) ◽  
pp. 243-247
Author(s):  
June-Hyung Kim ◽  
Soo-Keun Choi ◽  
Heung-Chae Jung ◽  
Jae-Gu Pan ◽  
Byung-Gee Kim
Keyword(s):  
Bacillus Subtilis ◽  
Zymomonas Mobilis ◽  
Surface Display ◽  
Subtilis Spore ◽  
Display System ◽  
Bacterial Surface ◽  
Bacillus Subtilis Spore ◽  
Bacterial Surface Display

scholarly journals Simultaneous Display of Multiple Foreign Peptides in the FliD Capping and FliC Filament Proteins of the Escherichia coli Flagellum

2005 ◽  
Vol 71 (8) ◽  
pp. 4263-4268 ◽  
Author(s):  
Katariina Majander ◽  
Timo K. Korhonen ◽  
Benita Westerlund-Wikström
Keyword(s):  
Surface Display ◽  
Variable Region ◽  
Biotechnological Applications ◽  
Display System ◽  
Bacterial Flagellum ◽  
Bacterial Surface ◽  
Capping Protein ◽  
Optimal Region ◽  
Bacterial Surface Display ◽  
Insertion Sites

ABSTRACT The bacterial flagellum is composed of more than 20 different proteins. The filament, which constitutes the major extracellular part of the flagellum, is built up of approximately 20,000 FliC molecules that assemble at the growing distal end of the filament. A capping structure composed of five FliD molecules located at the tip of the filament promotes polymerization of FliC. Lack of FliD leads to release of the subunits into the growth medium. We show here that FliD can be successfully used in bacterial surface display. We tested various insertion sites in the capping protein, and the optimal region for display was at the variable region in FliD. Deletion and/or insertion at other sites resulted in decreased formation of flagella. We further developed the technique into a multihybrid display system in which three foreign peptides are simultaneously expressed within the same flagellum, i.e., D repeats of FnBPA from Staphylococcus aureus at the tip and fragments of YadA from Yersinia enterocolitica as well as SlpA from Lactobacillus crispatus along the filament. This technology can have biotechnological applications, e.g., in simultaneous delivery of several effector molecules.

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