scholarly journals Human Serum Albumin as an Antioxidant in the Oxidation of (−)-Epigallocatechin Gallate: Participation of Reversible Covalent Binding for Interaction and Stabilization

2011 ◽  
Vol 75 (1) ◽  
pp. 100-106 ◽  
Author(s):  
Takeshi ISHII ◽  
Tatsuya ICHIKAWA ◽  
Kanako MINODA ◽  
Koji KUSAKA ◽  
Sohei ITO ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Covalent Binding ◽  
Epigallocatechin Gallate ◽  
Reversible Covalent

Reversible Covalent Binding of Neratinib to Human Serum Albumin In Vitro

2010 ◽  
Vol 4 (4) ◽  
pp. 220-227 ◽  
Author(s):  
Appavu Chandrasekaran ◽  
Li Shen ◽  
Susan Lockhead ◽  
Aram Oganesian ◽  
Jianyao Wang ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Covalent Binding ◽  
Reversible Covalent

RADIOIMMUNOASSAYS OF 3,5,3′-TRIIODO-L-THYRONINE WITH AND WITHOUT A PRIOR EXTRACTION STEP

1975 ◽  
Vol 80 (1) ◽  
pp. 58-69 ◽  
Author(s):  
A. Burger ◽  
C. Sakoloff ◽  
V. Staeheli ◽  
M. B. Vallotton ◽  
S. H. Ingbar
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Normal Values ◽  
Covalent Binding ◽  
Extraction Procedure ◽  
Mixed Population ◽  
Coupling Agents ◽  
Extraction Step ◽  
The Mean

ABSTRACT Two radioimmunoassays for triiodothyronine (T3) are described, one of which includes an extraction step, while the other does not. To raise antibodies, two carrier proteins and different coupling agents were used, namely haemocyanin and diazotized benzidine or human serum albumin and carbodiimide. In the case of T3 coupled to haemocyanin by diazotized benzidine, evidence of covalent binding of the hapten to the protein was obtained. In the case of T3 coupled to human serum albumin, little evidence of covalent linkage was available. Nevertheless immunization was successful in both cases. The radioimmunoassay in unextracted serum was highly reproducible and precise (intra-assay variability 5.2% inter-assay variability 8.1%). Normal values were determined which clearly indicate a fall in the serum T3 concentration with increasing age. In men the fall occurs in the fifth decade. In women the T3 starts to fall only after 70 years of age. In 31 cases of hyperthyroidism the serum T3 concentration ranged from 2.26 to 10.4 ng T3/ml. In 10 cases of hypothyroidism the values ranged from 0 to 0.8 ng T3/ml. The radioimmunoassay using an extraction procedure was less extensively used since it was found to be less reproducible (intra-assay variability 7.5%, inter-assay 12.25%). The normal values were determined with a mixed population aged 20–50. The mean ± 2 sd was 0.9 ± 0.36 ng T3/ml (n=52). In 17 cases of hypothyroidism the values ranged from 0 to 0.6 ng T3/ml and in 22 cases of hyperthyroidism from 2 to 14.4 ng T3/ml.

Kinetic Analysis of the Covalent Binding of Captopril to Human Serum Albumin

1997 ◽  
Vol 86 (2) ◽  
pp. 215-219 ◽  
Author(s):  
Ryuichi Narazaki ◽  
Kumiko Harada ◽  
Atsushi Sugii ◽  
Masaki Otagiri
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Kinetic Analysis ◽  
Covalent Binding

scholarly journals Covalent binding of cefotaxime to human serum albumin.

1983 ◽  
Vol 6 (2) ◽  
pp. 139-140 ◽  
Author(s):  
TOSHIMASA TOYO'OKA ◽  
AKIMITSU SANO ◽  
TAKEO KURIKI ◽  
NOBUO SUZUKI
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Covalent Binding

scholarly journals Photoactivated covalent binding of [3H]bilirubin to human serum albumin

1979 ◽  
Vol 181 (3) ◽  
pp. 779-781 ◽  
Author(s):  
D W Hutchinson ◽  
D S Mutopo
Keyword(s):  
Aqueous Solution ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Good Yield ◽  
Covalent Binding ◽  
Covalent Attachment ◽  
Step Procedure ◽  
One Step ◽  
Degradation Studies

A one-step procedure has been developed for the preparation of [3H]bilirubin IX-alpha in good yield from unlabelled bilirubin. Irradiation of an aqueous solution of [3H]bilirubin IX-alpha in the presence of human serum albumin results in the covalent attachment of the bilirubin to the protein. Preliminary degradation studies have been carried out to locate the site of attachment of the bilirubin to the albumin.

scholarly journals Photoinduced covalent binding of frusemide and frusemide glucuronide to human serum albumin

1999 ◽  
Vol 48 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Takashi Mizuma§ ◽  
Antony F. McDonagh ◽  
Emil T. Lin ◽  
Leslie Z. Benet
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Covalent Binding
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