scholarly journals Aspartyl Aminopeptidase, Encoded by an Evolutionarily Conserved Syntenic Gene, Is Colocalized with Its Cluster in Secretory Granules of Pancreatic Islet Cells

2010 ◽  
Vol 74 (10) ◽  
pp. 2050-2055 ◽  
Author(s):  
Wendy W. CAI ◽  
Lin WANG ◽  
Yuanxiu CHEN
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Secretory Granules ◽  
Pancreatic Islet Cells ◽  
Evolutionarily Conserved ◽  
Syntenic Gene ◽  
Aspartyl Aminopeptidase

scholarly journals Transport of ascorbic acid and dehydroascorbic acid by pancreatic islet cells from neonatal rats

1991 ◽  
Vol 274 (3) ◽  
pp. 739-744 ◽  
Author(s):  
A Zhou ◽  
J H Nielsen ◽  
O Farver ◽  
N A Thorn
Keyword(s):  
Ascorbic Acid ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Secretory Granules ◽  
Dehydroascorbic Acid ◽  
Pancreatic Tissue ◽  
Biologically Active ◽  
Pancreatic Islet Cells ◽  
High Concentration ◽  
Old Rats

Several amidated biologically active peptides such as pancreastatin, thyrotropin-releasing hormone, pancreatic polypeptide and amylin are produced in endocrine pancreatic tissue which contains the enzyme necessary for their final processing, i.e. peptidylglycine alpha-amidating mono-oxygenase (EC 1.14.17.3). The enzyme needs ascorbic acid for activity as well as copper and molecular oxygen. The present work shows that pancreatic islet cells prepared from overnight cultures of isolated islets from 5-7-day-old rats accumulate 14C-labelled ascorbic acid by a Na(+)-dependent active transport mechanism which involves a saturable process (estimated Km 17.6 microM). Transport was inhibited by ouabain, phloridzin, cytochalasin B, amiloride and probenecid. Glucose inhibited or stimulated uptake, depending on the length of incubation time of the cells. The uptake of dehydroascorbic acid was linearly dependent on concentration. Dehydroascorbic acid was converted to ascorbic acid by an unknown mechanism after uptake. The uptake of both ascorbic acid and dehydroascorbic acid was inhibited by tri-iodothyronine, and uptake of ascorbic acid, but not of dehydroascorbic acid, was inhibited by glucocorticoids. Isolated secretory granules contained a fairly low concentration of iron but a high concentration of copper.

scholarly journals Menin Immunoreactivity in Secretory Granules of Human Pancreatic Islet Cells

2014 ◽  
Vol 22 (10) ◽  
pp. 748-755 ◽  
Author(s):  
Larisa V. Debelenko ◽  
Sunita Agarwal ◽  
Qiang Du ◽  
Wusheng Yan ◽  
Heidi S. Erickson ◽  
...  
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Secretory Granules ◽  
Pancreatic Islet Cells ◽  
Human Pancreatic Islet

Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts

Diabetes ◽  
1980 ◽  
Vol 29 (6) ◽  
pp. 497-500 ◽  
Author(s):  
P. Meda ◽  
E. L. Hooghe-Peters ◽  
L. Orci
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Monolayer Cultures

Use of liposomes to introduce substances into pancreatic islet cells

Diabetes ◽  
1988 ◽  
Vol 37 (8) ◽  
pp. 1123-1128
Author(s):  
N. Welsh ◽  
A. Hallberg ◽  
S. Sandler ◽  
C. Hellerstrom
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Superfusion of dissociated pancreatic islet cells attached to Cytodex beads

Diabetes ◽  
1982 ◽  
Vol 31 (3) ◽  
pp. 189-193 ◽  
Author(s):  
Y. Spiess ◽  
M. A. Smith ◽  
W. Vale
Keyword(s):  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells

Efficient gene transfer to dispersed human pancreatic islet cells in vitro using adenovirus-polylysine/DNA complexes or polycationic liposomes

Diabetes ◽  
1996 ◽  
Vol 45 (9) ◽  
pp. 1197-1203 ◽  
Author(s):  
J. Saldeen ◽  
D. T. Curiel ◽  
D. L. Eizirik ◽  
A. Andersson ◽  
E. Strandell ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Pancreatic Islet ◽  
Islet Cells ◽  
Pancreatic Islet Cells ◽  
Dna Complexes ◽  
Human Pancreatic Islet ◽  
Efficient Gene
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