Light Stimulation Triggered Expression of Genes Coding for Vacuolar Proton-Pump Enzymes V-ATPase and V-PPase in Buckwheat

2010 ◽  
Vol 74 (7) ◽  
pp. 1507-1511 ◽  
Author(s):  
Masanobu KOJIMA ◽  
Yoko NAKANO ◽  
Hiroshi FUJII
Keyword(s):  
Proton Pump ◽  
Light Stimulation ◽  
Expression Of Genes ◽  
Vacuolar Proton Pump

scholarly journals Regulatory assembly of the vacuolar proton pump V o V 1 -ATPase in yeast cells by FLIM-FRET

2010 ◽  
Author(s):  
Stefan Ernst ◽  
Claire Batisse ◽  
Nawid Zarrabi ◽  
Bettina Böttcher ◽  
Michael Börsch
Keyword(s):  
Proton Pump ◽  
Yeast Cells ◽  
Vacuolar Proton Pump

The COOH termini of NBC3 and the 56-kDa H+-ATPase subunit are PDZ motifs involved in their interaction

2003 ◽  
Vol 284 (3) ◽  
pp. C667-C673 ◽  
Author(s):  
Alexander Pushkin ◽  
Natalia Abuladze ◽  
Debra Newman ◽  
Vladimir Muronets ◽  
Pejvak Sassani ◽  
...  
Keyword(s):  
Amino Acid ◽  
Proton Pump ◽  
Rat Kidney ◽  
Pdz Domain ◽  
Intercalated Cells ◽  
Regulatory Factor ◽  
Atpase Subunit ◽  
Sodium Bicarbonate Cotransporter ◽  
Terminal Amino ◽  
Vacuolar Proton Pump

The electroneutral sodium bicarbonate cotransporter 3 (NBC3) coimmunoprecipitates from renal lysates with the vacuolar H+-ATPase. In renal type A and B intercalated cells, NBC3 colocalizes with the vacuolar H+-ATPase. The involvement of the COOH termini of NBC3 and the 56-kDa subunit of the proton pump in the interaction of these proteins was investigated. The intact and modified COOH termini of NBC3 and the 56-kDa subunit of the proton pump were synthesized, coupled to Sepharose beads, and used to pull down kidney membrane proteins. Both the 56- and the 70-kDa subunits of the proton pump, as well as a PDZ domain containing protein Na+/H+ exchanger regulatory factor 1 (NHERF-1), were bound to the intact 18 amino acid NBC3 COOH terminus. A peptide truncated by five COOH-terminal amino acids did not bind these proteins. Replacement of the COOH-terminal leucine with glycine blocked binding of both the proton pump subunits but did not affect binding of NHERF-1. The 18 amino acid COOH terminus of the 56-kDa subunit of the proton pump bound NHERF-1 and NBC3, but the truncated and modified peptide did not. A complex of NBC3, the 56-kDa subunit of the proton pump, and NHERF-1 was identified in rat kidney. The data indicate that the COOH termini of NBC3 and the 56-kDa subunit of the vacuolar proton pump are PDZ-interacting motifs that are necessary for the interaction of these proteins. NHERF-1 is involved in the interaction of NBC3 and the vacuolar proton pump.

scholarly journals Effects of inhibitors of the vacuolar proton pump on hepatic heterophagy and autophagy

2001 ◽  
Vol 1510 (1-2) ◽  
pp. 243-257 ◽  
Author(s):  
Seyed Ali Mousavi ◽  
Rune Kjeken ◽  
Trond Olav Berg ◽  
Per Ottar Seglen ◽  
Trond Berg ◽  
...  
Keyword(s):  
Proton Pump ◽  
Vacuolar Proton Pump

scholarly journals Association of the 16-kDa Subunit c of Vacuolar Proton Pump with the Ileal Na+-dependent Bile Acid Transporter

2004 ◽  
Vol 279 (16) ◽  
pp. 16295-16300 ◽  
Author(s):  
An-Qiang Sun ◽  
Natarajan Balasubramaniyan ◽  
Chuan-Ju Liu ◽  
Mohammad Shahid ◽  
Frederick J. Suchy
Keyword(s):  
Bile Acid ◽  
Proton Pump ◽  
Subunit C ◽  
Bile Acid Transporter ◽  
Acid Transporter ◽  
Vacuolar Proton Pump

The vacuolar proton pump of Dictyostelium discoideum: molecular cloning and analysis of the 100 kDa subunit

1996 ◽  
Vol 109 (5) ◽  
pp. 1041-1051 ◽  
Author(s):  
T. Liu ◽  
M. Clarke
Keyword(s):  
Dictyostelium Discoideum ◽  
Proton Pump ◽  
Single Gene ◽  
Consensus Sequence ◽  
Amino Acid Sequences ◽  
Contractile Vacuole ◽  
Proton Pumps ◽  
Variable Regions ◽  
Endosomal Membranes ◽  
Vacuolar Proton Pump

The vacuolar proton pump is a highly-conserved multimeric enzyme that catalyzes the translocation of protons across the membranes of eukaryotic cells. Its largest subunit (95-116 kDa) occurs in tissue and organelle-specific isoforms and thus may be involved in targeting the enzyme or modulating its function. In amoebae of Dictyostelium discoideum, proton pumps with a 100 kDa subunit are found in membranes of the contractile vacuole complex, an osmoregulatory organelle. We cloned the cDNA that encodes this 100 kDa protein and found that its sequence predicts a protein 45% identical (68% similar) to the corresponding mammalian proton pump subunit. Like the mammalian protein, the predicted Dictyostelium sequence contains six possible transmembrane domains and a single consensus sequence for N-linked glycosylation. Southern blot analysis detected only a single gene, which was designated vatM. Using genomic DNA and degenerate oligonucleotides based on conserved regions of the protein as primers, we generated products by polymerase chain reaction that included highly variable regions of this protein family. The cloned products were identical in nucleotide sequence to vatM, arguing that Dictyostelium cells contain only a single isoform of this proton pump subunit. Consistent with this interpretation, the amino acid sequences of peptides derived from a protein associated with endosomal membranes (Adessu et al. (1995) J. Cell Sci. 108, 3331–3337) match the predicted sequence of the protein encoded by vatM. Thus, a single isoform of the 100 kDa proton pump subunit appears to serve in both the contractile vacuole system and the endosomal/lysosomal system of Dictyostelium, arguing that this subunit is not responsible for regulating the differing abundance and function of proton pumps in these two compartments. Gene targeting experiments suggest that this subunit plays important (possibly essential) roles in Dictyostelium cells.

Defects in TCIRG1 subunit of the vacuolar proton pump are responsible for a subset of human autosomal recessive osteopetrosis

10.1038/77131 ◽  
2000 ◽  
Vol 25 (3) ◽  
pp. 343-346 ◽  
Author(s):  
Annalisa Frattini ◽  
Paul J. Orchard ◽  
Cristina Sobacchi ◽  
Silvia Giliani ◽  
Mario Abinun ◽  
...  
Keyword(s):  
Proton Pump ◽  
Autosomal Recessive ◽  
Vacuolar Proton Pump ◽  
Autosomal Recessive Osteopetrosis

Novel mutations in theTCIRG1gene encoding the a3 subunit of the vacuolar proton pump in patients affected by infantile malignant osteopetrosis

2003 ◽  
Vol 21 (2) ◽  
pp. 151-157 ◽  
Author(s):  
Jean-Claude Scimeca ◽  
Danielle Quincey ◽  
Hugues Parrinello ◽  
Delphine Romatet ◽  
Josiane Grosgeorge ◽  
...  
Keyword(s):  
Proton Pump ◽  
Novel Mutations ◽  
Malignant Osteopetrosis ◽  
A3 Subunit ◽  
Infantile Malignant Osteopetrosis ◽  
Vacuolar Proton Pump
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