Molecular Characterization of Two Genes with High Similarity to the Dihydroxyacetone Synthase Gene in the Methylotrophic YeastPichia methanolica

2010 ◽  
Vol 74 (7) ◽  
pp. 1491-1493 ◽  
Author(s):  
Tomoyuki NAKAGAWA ◽  
Shuki FUJIMURA ◽  
Takashi ITO ◽  
Yoshimi MATSUFUJI ◽  
Shotaro OZAWA ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
High Similarity ◽  
Dihydroxyacetone Synthase

scholarly journals Belo Horizonte virus: a vaccinia-like virus lacking the A-type inclusion body gene isolated from infected mice

2004 ◽  
Vol 85 (7) ◽  
pp. 2015-2021 ◽  
Author(s):  
Giliane S. Trindade ◽  
Flávio G. da Fonseca ◽  
João T. Marques ◽  
Sueli Diniz ◽  
Juliana A. Leite ◽  
...  
Keyword(s):  
Growth Factor ◽  
Thymidine Kinase ◽  
Molecular Characterization ◽  
Inclusion Body ◽  
High Similarity ◽  
Cross Hybridization ◽  
Naturally Occurring ◽  
Belo Horizonte ◽  
Type Inclusion

Here is described the isolation of a naturally occurring A-type inclusion body (ATI)-negative vaccinia-like virus, Belo Horizonte virus (VBH), obtained from a mousepox-like outbreak in Brazil. The isolated virus was identified and characterized as an orthopoxvirus by conventional methods. Molecular characterization of the virus was done by DNA cross-hybridization using Vaccinia virus (VACV) DNA. In addition, conserved orthopoxvirus genes such as vaccinia growth factor, thymidine kinase and haemagglutinin were amplified by PCR and sequenced. All sequences presented high similarity to VACV genes. Based on the sequences, phenograms were constructed for comparison with other poxviruses; VBH clustered consistently with VACV strains. Attempts to amplify the ATI gene (ati) by PCR, currently used to identify orthopoxviruses, were unsuccessful. Results presented here suggest that most of the ati gene is deleted in the VBH genome.

scholarly journals Molecular characterization of potato cultivars using SSR markers

2011 ◽  
Vol 29 (4) ◽  
pp. 542-547 ◽  
Author(s):  
Patrícia Favoretto ◽  
Elizabeth Ann Veasey ◽  
Paulo César Tavares de Melo
Keyword(s):  
Molecular Characterization ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Binary Data ◽  
Potato Crop ◽  
Similarity Coefficient ◽  
High Similarity ◽  
Commercial Cultivars ◽  
Simple Sequence

The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified.

1446: Molecular Characterization of Cyclooxygenase-2 in a Stimulated Prostate Stroma Cells

2006 ◽  
Vol 175 (4S) ◽  
pp. 467-467
Author(s):  
Victor K. Lin ◽  
Shih-Ya Wang ◽  
Claus G. Roehrbom
Keyword(s):  
Molecular Characterization ◽  
Cyclooxygenase 2 ◽  
Prostate Stroma

Molecular characterization of lincRNAs in Down syndrome associated leukemia

2012 ◽  
Vol 224 (03) ◽  
Author(s):  
A Streltsov ◽  
S Emmrich ◽  
F Engeland ◽  
JH Klusmann
Keyword(s):  
Down Syndrome ◽  
Molecular Characterization

Molecular Characterization of Radiation-induced Glioblastoma

2018 ◽  
Author(s):  
MY Deng ◽  
D Sturm ◽  
E Pfaff ◽  
GP Balasubrama ◽  
J Schittenhelm ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Radiation Induced
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