scholarly journals Identification of a Proteolytic Bacterium, HW08, and Characterization of Its Extracellular Cold-Active Alkaline Metalloprotease Ps5

2010 ◽  
Vol 74 (6) ◽  
pp. 1220-1225 ◽  
Author(s):  
Chengye YANG ◽  
Fang WANG ◽  
Jianhua HAO ◽  
Kai ZHANG ◽  
Na YUAN ◽  
...  
Keyword(s):  
Cold Active ◽  
Proteolytic Bacterium

scholarly journals Isolation and characterization of a new cold-active protease from psychrotrophic bacteria of Western Himalayan glacial soil

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Saleem Farooq ◽  
Ruqeya Nazir ◽  
Shabir Ahmad Ganai ◽  
Bashir Ahmad Ganai
Keyword(s):  
Specific Activity ◽  
Temperature Range ◽  
Psychrotrophic Bacteria ◽  
Isolation And Characterization ◽  
Cold Active ◽  
Active Protease ◽  
Quantitative Screening ◽  
First Time ◽  
Low K

AbstractAs an approach to the exploration of cold-active enzymes, in this study, we isolated a cold-active protease produced by psychrotrophic bacteria from glacial soils of Thajwas Glacier, Himalayas. The isolated strain BO1, identified as Bacillus pumilus, grew well within a temperature range of 4–30 °C. After its qualitative and quantitative screening, the cold-active protease (Apr-BO1) was purified. The Apr-BO1 had a molecular mass of 38 kDa and showed maximum (37.02 U/mg) specific activity at 20 °C, with casein as substrate. It was stable and active between the temperature range of 5–35 °C and pH 6.0–12.0, with an optimum temperature of 20 °C at pH 9.0. The Apr-BO1 had low Km value of 1.0 mg/ml and Vmax 10.0 µmol/ml/min. Moreover, it displayed better tolerance to organic solvents, surfactants, metal ions and reducing agents than most alkaline proteases. The results exhibited that it effectively removed the stains even in a cold wash and could be considered a decent detergent additive. Furthermore, through protein modelling, the structure of this protease was generated from template, subtilisin E of Bacillus subtilis (PDB ID: 3WHI), and different methods checked its quality. For the first time, this study reported the protein sequence for psychrotrophic Apr-BO1 and brought forth its novelty among other cold-active proteases.

Characterization of a cold-active lipase from Psychrobacter cryohalolentis K5T and its deletion mutants

2013 ◽  
Vol 78 (4) ◽  
pp. 385-394 ◽  
Author(s):  
K. A. Novototskaya-Vlasova ◽  
L. E. Petrovskaya ◽  
E. M. Rivkina ◽  
D. A. Dolgikh ◽  
M. P. Kirpichnikov
Keyword(s):  
Deletion Mutants ◽  
Cold Active ◽  
Active Lipase

scholarly journals Identification and Characterization of a Halotolerant, Cold-Active Marine Endo-β-1,4-Glucanase by Using Functional Metagenomics of Seaweed-Associated Microbiota

2014 ◽  
Vol 80 (16) ◽  
pp. 4958-4967 ◽  
Author(s):  
Marjolaine Martin ◽  
Sophie Biver ◽  
Sébastien Steels ◽  
Tristan Barbeyron ◽  
Murielle Jam ◽  
...  
Keyword(s):  
Metagenomic Library ◽  
Functional Screening ◽  
Sample Collection ◽  
Prolonged Incubation ◽  
Content Type ◽  
Cold Active ◽  
Esterase Loci ◽  
Collection Period ◽  
Identification And Characterization

ABSTRACTA metagenomic library was constructed from microorganisms associated with the brown algaAscophyllum nodosum. Functional screening of this library revealed 13 novel putative esterase loci and two glycoside hydrolase loci. Sequence and gene cluster analysis showed the wide diversity of the identified enzymes and gave an idea of the microbial populations present during the sample collection period. Lastly, an endo-β-1,4-glucanase having less than 50% identity to sequences of known cellulases was purified and partially characterized, showing activity at low temperature and after prolonged incubation in concentrated salt solutions.

scholarly journals Crystal structure and functional characterization of a cold-active acetyl xylan esterase (PbAcE) from psychrophilic soil microbe Paenibacillus sp.

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0206260 ◽  
Author(s):  
Sun-Ha Park ◽  
Wanki Yoo ◽  
Chang Woo Lee ◽  
Chang Sook Jeong ◽  
Seung Chul Shin ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Functional Characterization ◽  
Acetyl Xylan Esterase ◽  
Soil Microbe ◽  
Paenibacillus Sp ◽  
Cold Active

Characterization of cold-active uracil-DNA glycosylase from Bacillus sp. HJ171 and its use for contamination control in PCR

2008 ◽  
Vol 80 (5) ◽  
pp. 785-794 ◽  
Author(s):  
Gun A. Kim ◽  
Mi Sun Lee ◽  
Younguk Sun ◽  
Byung Doo Lee ◽  
Jong Il Lee ◽  
...  
Keyword(s):  
Dna Glycosylase ◽  
Bacillus Sp ◽  
Uracil Dna Glycosylase ◽  
Contamination Control ◽  
Cold Active

Purification and characterization of a cold-active protease from psychrotrophicSerratia marcescensAP3801

1997 ◽  
Vol 74 (11) ◽  
pp. 1377-1383 ◽  
Author(s):  
Yasutaka Morita ◽  
Kenji Kondoh ◽  
Quamrul Hasan ◽  
Toshifumi Sakaguchi ◽  
Yuji Murakami ◽  
...  
Keyword(s):  
Purification And Characterization ◽  
Cold Active ◽  
Active Protease

Purification and characterization of cold-active endo-1,4-β-glucanase produced by Pseudoalteromonas sp. AN545 from Antarctica

2011 ◽  
Vol 29 (5) ◽  
pp. 1086-1092
Author(s):  
Jihong Shen ◽  
Guangfeng Kan ◽  
Cuijuan Shi ◽  
Zhenhuan Lei ◽  
Qiuju Xie ◽  
...  
Keyword(s):  
Purification And Characterization ◽  
Cold Active
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