scholarly journals Detection of epistatic interaction of two QTLs, gw8.1 and gw9.1, underlying grain weight using nearly isogenic lines in rice

2011 ◽  
Vol 61 (1) ◽  
pp. 69-75 ◽  
Author(s):  
Feng-Xue Jin ◽  
Shi-Dong Ji ◽  
Xiao-Bo Xie ◽  
Ju-Won Kang ◽  
Hong-Guang Ju ◽  
...  
Keyword(s):  
Epistatic Interaction ◽  
Grain Weight ◽  
Isogenic Lines ◽  
Nearly Isogenic Lines

scholarly journals Abiotic Stress Response of Near-Isogenic Spring Durum Wheat Lines under Different Sowing Densities

2021 ◽  
Vol 22 (4) ◽  
pp. 2053
Author(s):  
Judit Bányai ◽  
Marco Maccaferri ◽  
László Láng ◽  
Marianna Mayer ◽  
Viola Tóth ◽  
...  
Keyword(s):  
Drought Stress ◽  
Durum Wheat ◽  
Chlorophyll Content ◽  
Vegetation Index ◽  
Flag Leaf ◽  
Kernel Weight ◽  
Grain Weight ◽  
Abiotic Stress Response ◽  
Grain Number ◽  
Isogenic Lines

A detailed study was made of changes in the plant development, morphology, physiology and yield biology of near-isogenic lines of spring durum wheat sown in the field with different plant densities in two consecutive years (2013–2014). An analysis was made of the drought tolerance of isogenic lines selected for yield QTLs (QYld.idw-2B and QYld.idw-3B), and the presence of QTL effects was examined in spring sowings. Comparisons were made of the traits of the isogenic pairs QYld.idw-3B++ and QYld.idw-3B−− both within and between the pairs. Changes in the polyamine content, antioxidant enzyme activity, chlorophyll content of the flag leaf and the normalized difference vegetation index (NDVI) of the plot were monitored in response to drought stress, and the relationship between these components and the yield was analyzed. In the case of moderate stress, differences between the NIL++ and NIL−− pairs appeared in the early dough stage, indicating that the QYld.idw-3B++ QTL region was able to maintain photosynthetic activity for a longer period, resulting in greater grain number and grain weight at the end of the growing period. The chlorophyll content of the flag leaf in phenophases Z77 and Z83 was significantly correlated with the grain number and grain weight of the main spike. The grain yield was greatly influenced by the treatment, while the genotype had a significant effect on the thousand-kernel weight and on the grain number and grain weight of the main spike. When the lines were compared in the non-irrigated treatment, significantly more grains and significantly higher grain weight were observed in the main spike in NIL++ lines, confirming the theory that the higher yields of the QYld.idw-3B++ lines when sown in spring and exposed to drought stress could be attributed to the positive effect of the “Kofa” QTL on chromosome 3B.

scholarly journals Analysis of QTL Interaction for Grain Weight using Near Isogenic Lines in Rice

2015 ◽  
Vol 3 (1) ◽  
pp. 30-38 ◽  
Author(s):  
Hae-hwang Kim ◽  
Dong-min Kim ◽  
Ju-won Kang ◽  
Hyun-Sook Lee ◽  
Yun-ju Kang ◽  
...  
Keyword(s):  
Grain Weight ◽  
Near Isogenic Lines ◽  
Isogenic Lines

scholarly journals Ubiquitin-related genes are differentially expressed in isogenic lines contrasting for pericarp cell size and grain weight in hexaploid wheat

2017 ◽  
Author(s):  
Jemima Brinton ◽  
James Simmonds ◽  
Cristobal Uauy
Keyword(s):  
Cell Size ◽  
Crop Yields ◽  
Grain Weight ◽  
Differentially Expressed ◽  
Reference Sequence ◽  
Complex Nature ◽  
Grain Development ◽  
Diverse Range ◽  
Isogenic Lines ◽  
Gene Models

AbstractBackgroundThere is an urgent need to increase global crop production. Identifying and combining genes controlling indidviual yield components, such as grain weight, holds the potential to enhance crop yields. Transcriptomics is a powerful tool to gain insights into the complex gene regulatory networks that underlie such traits, but relies on the availability of a high-quality reference sequence and accurate gene models. Previously, we identified a grain weight QTL on wheat chromosome 5A (5A QTL) which acts during early grain development to increase grain length through cell expansion in the pericarp. In this study, we performed RNA-sequencing on near isogenic lines (NILs) segregating for the 5A QTL and used the latest gene models to identify differentially expressed (DE) genes and pathways that potentially influence pericarp cell size and grain weight in wheat.ResultsWe sampled grains at four and eight days post anthesis and found genes associated with metabolism, biosynthesis, proteoloysis and defence response to be upregulated during this stage of grain development in both NILs. We identified a specific set of 112 transcripts DE between 5A NILs at either time point, including seven potential candidates for the causal gene underlying the 5A QTL. The 112 DE transcripts had functional annotations including non-coding RNA, transpon-associated, cell-cycle control, and ubiquitin-related processes. Many of the wheat genes identified belong to families that have been previously associated with seed/grain development in other species. However, few of these wheat genes are the direct orthologs and none have been previously characterised in wheat. Notably, we identified DE transcripts at almost all steps of the pathway associated with ubiquitin-mediated protein degradation. In the promoters of a subset of DE transcripts we identified enrichment of binding sites associated with C2H2, MYB/SANT, YABBY, AT HOOK and Trihelix transcription factor families.ConclusionsIn this study, we identified DE transcripts with a diverse range of predicted biological functions, reflecting the complex nature of the pathways that control early grain development. Further functional characterisation of these candidates and how they interact could provide new insights into the control of grain size in cereals, ultimately improving crop yield.

Mapping QTLs for Grain Weight and Shape Using Four Sister Near Isogenic Lines of Rice

2010 ◽  
Vol 36 (8) ◽  
pp. 1310-1317
Author(s):  
Guo-Xin YAO ◽  
Jin-Jie LI ◽  
Qiang ZHANG ◽  
Guang-Long HU ◽  
Chao CHEN ◽  
...  
Keyword(s):  
Grain Weight ◽  
Near Isogenic Lines ◽  
Isogenic Lines

Construction and evaluation of near-isogenic lines for major QTLs of basal root thickness and 1000-grain-weight in lowland and upland rice

2007 ◽  
Vol 4 (3) ◽  
pp. 199-205 ◽  
Author(s):  
Liu Li-Feng ◽  
Zhang Hong-Liang ◽  
Mu Ping ◽  
Qu Yan-Ying ◽  
Li Zi-Chao
Keyword(s):  
Molecular Marker ◽  
Upland Rice ◽  
Grain Weight ◽  
Recurrent Parent ◽  
Near Isogenic Lines ◽  
Major Qtls ◽  
Isogenic Lines ◽  
Root Thickness ◽  
Selection For ◽  
1000 Grain Weight

AbstractThe development of near-isogenic lines (NILs) is an important fundamental step in the cloning of quantitative trait loci (QTL) and molecular marker assisted breeding. In the present study, NILs for two major QTLs of basal root thickness (BRT) and 1000-grain-weight (TGW) were obtained by molecular marker-assisted selection (MAS) through foreground selection for target QTL and background selection in three backcross generations (BC1F1, BC2F1 and BC3F1). After phenotypic evaluation in the BC3F2 generation, nine BRT QTL-NILs with BRT ranges of 1.07–1.16 mm (6.11–15.18% greater than the recurrent parent) and an average recovery ratio of genetic background (RRGB) of 97.22%, and 11 TGW QTL-NILs with ranges of 21.25–26.25 g (7.05–32.16% greater than the recurrent parent) and 95.97% of RRGB, were selected.

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