scholarly journals Biological nitrification inhibition (BNI)—Is there potential for genetic interventions in the Triticeae?

2009 ◽  
Vol 59 (5) ◽  
pp. 529-545 ◽  
Author(s):  
Guntur Venkata Subbarao ◽  
Masahiro Kishii ◽  
Kazuhiko Nakahara ◽  
Takayuki Ishikawa ◽  
Tomohiro Ban ◽  
...  
Keyword(s):  
Nitrification Inhibition ◽  
Biological Nitrification ◽  
Genetic Interventions

scholarly journals Biological nitrification inhibition in maize—isolation and identification of hydrophobic inhibitors from root exudates

2021 ◽  
Author(s):  
Junnosuke Otaka ◽  
Guntur Venkata Subbarao ◽  
Hiroshi Ono ◽  
Tadashi Yoshihashi
Keyword(s):  
Root Exudation ◽  
Root Systems ◽  
Root Surface ◽  
Maize Root ◽  
Nitrification Inhibition ◽  
Isolation And Identification ◽  
N Losses ◽  
Chemical Structures ◽  
Maize Roots ◽  
Biological Nitrification

AbstractTo control agronomic N losses and reduce environmental pollution, biological nitrification inhibition (BNI) is a promising strategy. BNI is an ecological phenomenon by which certain plants release bioactive compounds that can suppress nitrifying soil microbes. Herein, we report on two hydrophobic BNI compounds released from maize root exudation (1 and 2), together with two BNI compounds inside maize roots (3 and 4). On the basis of a bioassay-guided fractionation method using a recombinant nitrifying bacterium Nitrosomonas europaea, 2,7-dimethoxy-1,4-naphthoquinone (1, ED50 = 2 μM) was identified for the first time from dichloromethane (DCM) wash concentrate of maize root surface and named “zeanone.” The benzoxazinoid 2-hydroxy-4,7-dimethoxy-2H-1,4-benzoxazin-3(4H)-one (HDMBOA, 2, ED50 = 13 μM) was isolated from DCM extract of maize roots, and two analogs of compound 2, 2-hydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (HMBOA, 3, ED50 = 91 μM) and HDMBOA-β-glucoside (4, ED50 = 94 μM), were isolated from methanol extract of maize roots. Their chemical structures (1–4) were determined by extensive spectroscopic methods. The contributions of these four isolated BNI compounds (1–4) to the hydrophobic BNI activity in maize roots were 19%, 20%, 2%, and 4%, respectively. A possible biosynthetic pathway for zeanone (1) is proposed. These results provide insights into the strength of hydrophobic BNI activity released from maize root systems, the chemical identities of the isolated BNIs, and their relative contribution to the BNI activity from maize root systems.

scholarly journals Biological nitrification inhibition activity in a soil-grown biparental population of the forage grass, Brachiaria humidicola

2018 ◽  
Vol 426 (1-2) ◽  
pp. 401-411 ◽  
Author(s):  
Jonathan Nuñez ◽  
Ashly Arevalo ◽  
Hannes Karwat ◽  
Konrad Egenolf ◽  
John Miles ◽  
...  
Keyword(s):  
Forage Grass ◽  
Nitrification Inhibition ◽  
Inhibition Activity ◽  
Brachiaria Humidicola ◽  
Biparental Population ◽  
Biological Nitrification

Biological nitrification inhibition by weeds: wild radish, brome grass, wild oats and annual ryegrass decrease nitrification rates in their rhizospheres

2017 ◽  
Vol 68 (8) ◽  
pp. 798 ◽  
Author(s):  
Cathryn A. O'Sullivan ◽  
Kelley Whisson ◽  
Karen Treble ◽  
Margaret M. Roper ◽  
Shayne F. Micin ◽  
...  
Keyword(s):  
Root Exudates ◽  
Pure Culture ◽  
Nitrification Inhibition ◽  
Annual Ryegrass ◽  
Wild Radish ◽  
Weed Species ◽  
Wild Oats ◽  
Biological Nitrification ◽  
Brome Grass ◽  
The Impact

This study investigated the ability of several plant species commonly occurring as weeds in Australian cropping systems to produce root exudates that inhibit nitrification via biological nitrification inhibition (BNI). Seedlings of wild radish (Raphanus raphanistrum), great brome grass (Bromus diandrus), wild oats (Avena fatua), annual ryegrass (Lolium rigidum) and Brachiaria humidicola (BNI-positive control) were grown in hydroponics, and the impact of their root exudates on NO3– production by Nitrosomonas europaea was measured in a pure-culture assay. A pot study (soil-based assay) was then conducted to confirm the ability of the weeds to inhibit nitrification in whole soils. All of the tested weeds slowed NO3– production by N. europaea in the pure-culture assay and significantly inhibited potential nitrification rates in soil-based assays. Root exudates produced by wild radish were the most inhibitory, slowing NO3– production by the pure culture of N. europaea by 53 ± 6.1% and completely inhibiting nitrification in the soil-based assay. The other weed species all had BNI capacities comparable to that of B. humidicola and significantly higher than that previously reported for wheat cv. Janz. This study demonstrates that several commonly occurring weed species have BNI capacity. By altering the N cycle, and retaining NH4+ in the soils in which they grow, these weeds may gain a competitive advantage over species (including crops) that prefer NO3–. Increasing our understanding of how weeds compete with crops for N may open avenues for novel weed-management strategies.

NH 4 + triggers the synthesis and release of biological nitrification inhibition compounds in Brachiaria humidicola roots

2006 ◽  
Vol 290 (1-2) ◽  
pp. 245-257 ◽  
Author(s):  
G. V. Subbarao ◽  
H. Y. Wang ◽  
O. Ito ◽  
K. Nakahara ◽  
W. L. Berry
Keyword(s):  
Nitrification Inhibition ◽  
Brachiaria Humidicola ◽  
Biological Nitrification

scholarly journals Biological nitrification inhibition and forage productivity of Megathyrsus maximus in Colombian dry tropics

2021 ◽  
Author(s):  
Juliana Carvajal-Tapia ◽  
Sandra Morales Velasco ◽  
Daniel M. Villegas ◽  
Jacobo Arango ◽  
Nelson José Vivas Quila
Keyword(s):  
Dry Matter ◽  
High Capacity ◽  
Quality Parameters ◽  
Nitrification Inhibition ◽  
Soil Nitrification ◽  
Nutrition Quality ◽  
Dry Tropics ◽  
Biological Nitrification ◽  
Grassland Production ◽  
Germplasm Accessions

Agronomic, nutritional, and environmental aspects are integrated to promote sustainable tropical grassland production. Biological nitrification inhibition (BNI) is a plant-based strategy to improve nitrogen use efficiency by grasses in which they suppress the pace of soil nitrification via exudation of inhibitory compounds. To evaluate the effect of BNI on the productive performance of Megathyrsus maximus under field conditions, we evaluated a collection of 27 germplasm accessions and commercial cultivars of the forage grass in the dry tropics of Colombia. We measured plant yield dry matter, nutrition quality parameters, and nitrification rates of soil at 22 months after pasture establishment. Our results highlighted germplasm accessions of superior agronomic performance (for dry matter production and nutrition quality) and high capacity to decrease nitrification. Although no relation was observed between agronomic aspects, nutritional aspects, and nitrification rates, we conclude that there is no agronomic or nutritional penalty on environmentally friendly grasses, and BNI could be adopted as a target trait in plant breeding programs toward the development of eco-efficient forages and contribute to the sustainable intensification of livestock systems.  

scholarly journals Developing methods to evaluate phenotypic variability in biological nitrification inhibition (BNI) capacity of Brachiaria grasses

2014 ◽  
Vol 2 (1) ◽  
pp. 6 ◽  
Author(s):  
Jacobo Arango ◽  
Danilo Moreta ◽  
Jonathan Núñez ◽  
Katharina Hartmann ◽  
Moralba Domínguez ◽  
...  
Keyword(s):  
Phenotypic Variability ◽  
Nitrification Inhibition ◽  
Biological Nitrification

scholarly journals Biological nitrification inhibition by root exudates of native species,Hibiscus splendensandSolanum echinatum

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4960 ◽  
Author(s):  
Chelsea K. Janke ◽  
Laura A. Wendling ◽  
Ryosuke Fujinuma
Keyword(s):  
Plant Species ◽  
Root Exudates ◽  
Native Species ◽  
N Uptake ◽  
Nitrification Inhibition ◽  
Native Plant ◽  
Native Plant Species ◽  
Potential Nitrification ◽  
Australian Native Plant ◽  
Biological Nitrification

Australian native species grow competitively in nutrient limited environments, particularly in nitrogen (N) limited soils; however, the mechanism that enables this is poorly understood. Biological nitrification inhibition (BNI), which is the release of root exudates into the plant rhizosphere to inhibit the nitrification process, is a hypothesized adaptive mechanism for maximizing N uptake. To date, few studies have investigated the temporal pattern and components of root exudates by Australian native plant species for BNI. This study examined root exudates from two Australian native species,Hibiscus splendensandSolanum echinatum,and contrasted with exudates ofSorghum bicolor, a plant widely demonstrated to exhibit BNI capacity. Root exudates were collected from plants at two, four, and six weeks after transplanting to solution culture. Root exudates contained three types of organic acids (OAs), oxalic, citric and succinic acids, regardless of the species. However, the two Australian natives species released larger amount of OAs in earlier development stages thanS. bicolor. The total quantity of these OAs released per unit root dry mass was also seven-ten times greater for Australian native plant species compared toS. bicolor. The root exudates significantly inhibited nitrification activity over six weeks’ growth in a potential nitrification assay, withS. echinatum(ca. 81% inhibition) >S. bicolor(ca. 80% inhibition) >H. splendens(ca. 78% inhibition). The narrow range of BNI capacity in the study plants limited the determination of a relationship between OAs and BNI; however, a lack of correlation between individual OAs and inhibition of nitrification suggests OAs may not directly contribute to BNI. These results indicate that Australian native species generate a strongly N conserving environment within the rhizosphere up to six weeks after germination, establishing a competitive advantage in severely N limited environments.

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