scholarly journals Restricted distribution of orf687 as the pollen fertility restorer gene for Ogura male sterility in Japanese wild radish

2008 ◽  
Vol 58 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Keita Yasumoto ◽  
Yoshitaka Matsumoto ◽  
Toru Terachi ◽  
Hiroshi Yamagishi
Keyword(s):  
Male Sterility ◽  
Pollen Fertility ◽  
Restorer Gene ◽  
Wild Radish ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Restricted Distribution ◽  
Ogura Male Sterility ◽  
Pollen Fertility Restorer

scholarly journals A single nucleotide substitution in the coding region of Ogura male sterile gene, orf138, determines effectiveness of a fertility restorer gene, Rfo, in radish

2021 ◽  
Author(s):  
Hiroshi Yamagishi ◽  
Megumi Jikuya ◽  
Kanako Okushiro ◽  
Ayako Hashimoto ◽  
Asumi Fukunaga ◽  
...  
Keyword(s):  
Male Sterility ◽  
Nucleotide Substitution ◽  
Fertility Restoration ◽  
Type A ◽  
Restorer Gene ◽  
Single Nucleotide Substitution ◽  
Coding Region ◽  
Fertility Restorer ◽  
Single Nucleotide ◽  
Fertility Restorer Gene

AbstractCytoplasmic male sterility (CMS) observed in many plants leads defect in the production of functional pollen, while the expression of CMS is suppressed by a fertility restorer gene in the nuclear genome. Ogura CMS of radish is induced by a mitochondrial orf138, and a fertility restorer gene, Rfo, encodes a P-type PPR protein, ORF687, acting at the translational level. But, the exact function of ORF687 is still unclear. We found a Japanese variety showing male sterility even in the presence of Rfo. We examined the pollen fertility, Rfo expression, and orf138 mRNA in progenies of this variety. The progeny with Type H orf138 and Rfo showed male sterility when their orf138 mRNA was unprocessed within the coding region. By contrast, all progeny with Type A orf138 were fertile though orf138 mRNA remained unprocessed in the coding region, demonstrating that ORF687 functions on Type A but not on Type H. In silico analysis suggested a specific binding site of ORF687 in the coding region, not the 5′ untranslated region estimated previously, of Type A. A single nucleotide substitution in the putative binding site diminishes affinity of ORF687 in Type H and is most likely the cause of the ineffectiveness of ORF687. Furthermore, fertility restoration by RNA processing at a novel site in some progeny plants indicated a new and the third fertility restorer gene, Rfs, for orf138. This study clarified that direct ORF687 binding to the coding region of orf138 is essential for fertility restoration by Rfo.

Inheritance of male sterility and presence of dominant fertility restorer gene in ridge gourd (Luffa acutangula (Roxb.) L.)

2012 ◽  
Vol 144 ◽  
pp. 60-64 ◽  
Author(s):  
T. Pradeepkumar ◽  
V.C. Hegade ◽  
D. Kannan ◽  
R. Sujatha ◽  
T.E. George ◽  
...  
Keyword(s):  
Male Sterility ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Fertility Restorer Gene ◽  
Luffa Acutangula

A novel Rf gene controlling fertility restoration of Ogura male sterility by RNA processing of orf138 found in Japanese wild radish and its STS markers

Genome ◽  
2009 ◽  
Vol 52 (6) ◽  
pp. 495-504 ◽  
Author(s):  
Keita Yasumoto ◽  
Toru Terachi ◽  
Hiroshi Yamagishi
Keyword(s):  
Male Sterility ◽  
Northern Blot ◽  
Blot Analysis ◽  
Pollen Fertility ◽  
Northern Blot Analysis ◽  
Fertility Restoration ◽  
Wild Radish ◽  
Male Sterile ◽  
Sts Markers ◽  
Ogura Male Sterility

To reveal the molecular and genetic mechanism of fertility restoration in Ogura male sterility in Japanese wild radish ( Raphanus sativus var. hortensis f. raphanistroides), we investigated fertility restoration of a plant that lacks the dominant type of orf687, a previously identified fertility restorer gene. A total of 100 F2 plants were made from the cross between a male-sterile strain with the Ogura cytoplasm, ‘MS-Gensuke’, and a Japanese wild radish plant. Segregation of pollen fertility in the F2 plants led us to assume that 2 dominant complementary genes controlled the fertility restoration of the plants. However, the fertility of 27 of 59 male-fertile plants was not completely restored, resulting in a group of plants with partial male fertility. Northern blot analysis of the CMS-associated gene orf138 indicated that one restorer allele (termed Rft) was involved in the processing of orf138 RNA. Rapid amplification of cDNA ends (RACE) and subsequent Northern blot analysis confirmed that the orf138 transcript lost a 5′ part of the coding region of the orf138 gene in the restored plants. The accumulation of ORF138 protein was significantly reduced by Rft, but trace amounts of the protein were recognized in both partially male-fertile and male-sterile plants with Rft. The relationship of pollen fertility and segregation of co-dominant sequence tagged site (STS) markers in the F2 generation suggested that the penetrance of Rft was so low that Rft needs suitable conditions to function sufficiently for the complete restoration of fertility.

Sign in / Sign up

Export Citation Format

Share Document