scholarly journals Partial Synchronization of Cell Division and Micronucleation in Suspension-cultured Cells of Hemerocallis hybrida: The effects of Hydroxyurea and Various Spindle Toxins.

2001 ◽  
Vol 51 (4) ◽  
pp. 285-291 ◽  
Author(s):  
Hiroyuki Saito ◽  
Masaru Nakano
Keyword(s):  
Cell Division ◽  
Cultured Cells ◽  
Partial Synchronization ◽  
Suspension Cultured Cells ◽  
Synchronization Of Cell Division

Partial synchronization of cell division in root meristem induced by 5-aminouracil

1979 ◽  
Vol 21 (1) ◽  
pp. 51-56 ◽  
Author(s):  
F. J. Novák ◽  
Květuše Schwammenhöferová ◽  
Jarmila Čihalíková ◽  
M. Ondřej
Keyword(s):  
Cell Division ◽  
Root Meristem ◽  
Partial Synchronization ◽  
Synchronization Of Cell Division

Changes in Fructose-2,6-bisphosphate Level during the Growth of Suspension Cultured Cells of Catharanthus roseus

1986 ◽  
Vol 41 (5-6) ◽  
pp. 529-531 ◽  
Author(s):  
Hiroshi Ashihara
Keyword(s):  
Cell Division ◽  
Suspension Culture ◽  
Carbohydrate Metabolism ◽  
Catharanthus Roseus ◽  
Cultured Cells ◽  
Lag Phase ◽  
Fresh Weight ◽  
Suspension Cultured Cells

The level of fructose-2,6-bisphosphate (F2,6BP), a potent regulator of carbohydrate metabolism , varied from 23 to 448 pmol·g fresh weight-1 during the growth of the cells of Catharanthusroseus in suspension culture. The maximum F2,6BP level was found in the cells at the lag phase of the growth, and its level decreased with cell division and enlargement.

Induction of freezing tolerance in potato (Solanum commersonii) suspension cultured cells

1992 ◽  
Vol 84 (1) ◽  
pp. 41-48 ◽  
Author(s):  
Stephen P. Lee ◽  
Baolong Zhu ◽  
Tony H. H. Chen ◽  
Paul H. Li
Keyword(s):  
Freezing Tolerance ◽  
Cultured Cells ◽  
Solanum Commersonii ◽  
Suspension Cultured Cells

Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

2020 ◽  
Author(s):  
Ian Sims ◽  
K Middleton ◽  
AG Lane ◽  
AJ Cairns ◽  
A Bacic
Keyword(s):  
Cultured Cells ◽  
Oryza Sativa L ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Phleum Pratense ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Type Ii ◽  
Hordeum Vulgare L ◽  
Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

Characterisation of extracellular polysaccharides from suspension cultures of members of the Poaceae

2020 ◽  
Author(s):  
Ian Sims ◽  
K Middleton ◽  
AG Lane ◽  
AJ Cairns ◽  
A Bacic
Keyword(s):  
Cultured Cells ◽  
Oryza Sativa L ◽  
Suspension Cultures ◽  
Exchange Chromatography ◽  
Phleum Pratense ◽  
Arabinogalactan Protein ◽  
Extracellular Polysaccharides ◽  
Type Ii ◽  
Hordeum Vulgare L ◽  
Suspension Cultured Cells

Microscopic examination of suspension-cultured cells of Phleum pratense L., Panicum miliaceum L., Phalaris aquatica L. and Oryza sativa L. showed that they were comprised of numerous root primordia. Polysaccharides secreted by these suspension cultures contained glycosyl linkages consistent with the presence of high proportions of root mucilage-like polysaccharides. In contrast, suspension-cultured cells of Hordeum vulgare L. contained mostly undifferentiated cells more typical of plant cells in suspension culture. The polysaccharides secreted by H. vulgare cultures contained mostly linkages consistent with the presence of glucuronoarabinoxylan. The soluble polymers secreted by cell-suspension cultures of Phleum pratense contained 70% carbohydrate, 14% protein and 6% inorganic material. The extracellular polysaccharides were separated into four fractions by anion-exchange chromatography using a gradient of imidazole-HCl at pH 7.0. From glycosyl-linkage analyses, five polysaccharides were identified: an arabinosylated xyloglucan (comprising 20% of the total polysaccharide), a glucomannan (6%), a type-II arabinogalactan (an arabinogalactan-protein; 7%), an acidic xylan (3%), and a root-slime-like polysaccharide, which contained features of type-II arabinogalactans and glucuronomannans (65%).

scholarly journals Transcriptional Response of Soybean Suspension-cultured Cells Induced by Nod Factors Obtained from Bradyrhizobium japonicum USDA110

2002 ◽  
Vol 43 (11) ◽  
pp. 1314-1322
Author(s):  
Tsuneo Hakoyama ◽  
Tadashi Yokoyama ◽  
Hiroshi Kouchi ◽  
Ken-ichi Tsuchiya ◽  
Hisatoshi Kaku ◽  
...  
Keyword(s):  
Bradyrhizobium Japonicum ◽  
Cultured Cells ◽  
Transcriptional Response ◽  
Nod Factors ◽  
Suspension Cultured Cells

Phytotropins. IV. Effect of Phytotropins on Cultured Plant Cells and Protoplasts

1982 ◽  
Vol 9 (3) ◽  
pp. 297 ◽  
Author(s):  
PJ Larkin ◽  
W Scowcroft ◽  
AE Geissler ◽  
GF Katekar
Keyword(s):  
Petunia Hybrida ◽  
Cultured Cells ◽  
Plant Cells ◽  
Dichlorophenoxyacetic Acid ◽  
Polar Transport ◽  
Suspension Cultured Cells ◽  
Specific Receptors ◽  
Nicotiana Debneyi ◽  
2,4 Dichlorophenoxyacetic Acid

The phytotropins l-(2'-carboxyphenyl)-3-phenylpropane-1,3-dione (CPP) and 5-(2-carboxyphenyl)- 3-phenylpyrazole (CPD) reduced the net efflux of radiolabel from suspension-cultured cells treated with [14C]2,4-dichlorophenoxyacetic acid when present at concentrations comparable to those that inhibit polar transport of auxins in bean petioles. These phytotropins stimulated division of protoplasts of both Nicotiana debneyi and Petunia hybrida at concentrations of exogenous auxins that were otherwise suboptimal for divisions. The results are consistent with the proposal that phytotropins interact with specific receptors to reduce auxin efflux, resulting in increased intracellular auxin concentrations.

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