Changes in Anthocyanin Content and Composition of Developing Storage Root of Purple-Fleshed Sweet Potato(Ipomoea batatas(L.) Lam).

2000 ◽  
Vol 50 (1) ◽  
pp. 59-64 ◽  
Author(s):  
Masaru Yoshinaga ◽  
Masaru Tanaka ◽  
Makoto Nakatani
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Anthocyanin Content ◽  
Storage Root

scholarly journals The Expression of IbMYB1 Is Essential to Maintain the Purple Color of Leaf and Storage Root in Sweet Potato [Ipomoea batatas (L.) Lam]

2021 ◽  
Vol 12 ◽  
Author(s):  
Daowei Zhang ◽  
Yongjun Tan ◽  
Fang Dong ◽  
Ya Zhang ◽  
Yanlan Huang ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Anthocyanin Biosynthesis ◽  
Expression Patterns ◽  
Anthocyanin Content ◽  
Storage Root ◽  
Storage Roots ◽  
Cultivated Species ◽  
Purple Color ◽  
And Storage

IbMYB1 was one of the major anthocyanin biosynthesis regulatory genes that has been identified and utilized in purple-fleshed sweet potato breeding. At least three members of this gene, namely, IbMYB1-1, -2a, and -2b, have been reported. We found that IbMYB1-2a and -2b are not necessary for anthocyanin accumulation in a variety of cultivated species (hexaploid) with purple shoots or purplish rings/spots of flesh. Transcriptomic and quantitative reverse transcription PCR (RT-qPCR) analyses revealed that persistent and vigorous expression of IbMYB1 is essential to maintain the purple color of leaves and storage roots in this type of cultivated species, which did not contain IbMYB1-2 gene members. Compared with IbbHLH2, IbMYB1 is an early response gene of anthocyanin biosynthesis in sweet potato. It cannot exclude the possibility that other MYBs participate in this gene regulation networks. Twenty-two MYB-like genes were identified from 156 MYBs to be highly positively or negatively correlated with the anthocyanin content in leaves or flesh. Even so, the IbMYB1 was most coordinately expressed with anthocyanin biosynthesis genes. Differences in flanking and coding sequences confirm that IbMYB2s, the highest similarity genes of IbMYB1, are not the members of IbMYB1. This phenomenon indicates that there may be more members of IbMYB1 in sweet potato, and the genetic complementation of these members is involved in the regulation of anthocyanin biosynthesis. The 3′ flanking sequence of IbMYB1-1 is homologous to the retrotransposon sequence of TNT1-94. Transposon movement is involved in the formation of multiple members of IbMYB1. This study provides critical insights into the expression patterns of IbMYB1, which are involved in the regulation of anthocyanin biosynthesis in the leaf and storage root. Notably, our study also emphasized the presence of a multiple member of IbMYB1 for genetic improvement.

scholarly journals Potassium Fertilization Stimulates Sucrose-to-Starch Conversion and Root Formation in Sweet Potato (Ipomoea batatas (L.) Lam.)

2021 ◽  
Vol 22 (9) ◽  
pp. 4826
Author(s):  
Yang Gao ◽  
Zhonghou Tang ◽  
Houqiang Xia ◽  
Minfei Sheng ◽  
Ming Liu ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Intercellular Co2 Concentration ◽  
Biomass Accumulation ◽  
Starch Accumulation ◽  
Storage Root ◽  
Root Yield ◽  
Starch Conversion ◽  
And Storage ◽  
Low K

A field experiment was established to study sweet potato growth, starch dynamic accumulation, key enzymes and gene transcription in the sucrose-to-starch conversion and their relationships under six K2O rates using Ningzishu 1 (sensitive to low-K) and Xushu 32 (tolerant to low-K). The results indicated that K application significantly improved the biomass accumulation of plant and storage root, although treatments at high levels of K, i.e., 300–375 kg K2O ha−1, significantly decreased plant biomass and storage root yield. Compared with the no-K treatment, K application enhanced the biomass accumulation of plant and storage root by 3–47% and 13–45%, respectively, through promoting the biomass accumulation rate. Additionally, K application also enhanced the photosynthetic capacity of sweet potato. In this study, low stomatal conductance and net photosynthetic rate (Pn) accompanied with decreased intercellular CO2 concentration were observed in the no-K treatment at 35 DAT, indicating that Pn was reduced mainly due to stomatal limitation; at 55 DAT, reduced Pn in the no-K treatment was caused by non-stomatal factors. Compared with the no-K treatment, the content of sucrose, amylose and amylopectin decreased by 9–34%, 9–23% and 6–19%, respectively, but starch accumulation increased by 11–21% under K supply. The activities of sucrose synthetase (SuSy), adenosine-diphosphate-glucose pyrophosphorylase (AGPase), starch synthase (SSS) and the transcription of Susy, AGP, SSS34 and SSS67 were enhanced by K application and had positive relationships with starch accumulation. Therefore, K application promoted starch accumulation and storage root yield through regulating the activities and genes transcription of SuSy, AGPase and SSS in the sucrose-to-starch conversion.

scholarly journals Characterization of Anthocyanins in Sweet Potato Leaves Grown in Various Stages and Conditions

2019 ◽  
pp. 253-262
Author(s):  
Xiaoyu Su ◽  
Zhenbao Jia ◽  
Fei Tao ◽  
Jiamin Shen ◽  
Jingwen Xu ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Anthocyanin Biosynthesis ◽  
Total Phenolic ◽  
Growth Stages ◽  
Anthocyanin Content ◽  
Potential Health ◽  
Significant Difference ◽  
Young Leaves ◽  
Sweet Potato Leaves

Phytochemical-enriched edible greens, sweet potato leaves (Ipomoea batatas L.), have become popular due to potential health benefits. However, the phytochemical contents in sweet potato leaves and their subsequent change over harvest stages and growth condition are mostly unknown. In this study, the anthocyanin profile and content in leaves of four sweet potato cultivars, i.e., white-skinned and white-fleshed Bonita, red-skinned and orange-fleshed Beauregard, red-skinned and white-fleshed Murasaki and purple-skinned and purple-fleshed P40, were evaluated. Fourteen anthocyanins were isolated and identified by HPLC-MSI/MS. The most abundant was cyanidin 3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, which comprised up to 20% of the total anthocyanins. Of the young leaves (1st and 2nd slip cuttings), Bonita contained the highest anthocyanin content followed by P40. Of the mature leaves (vine stage), Beauregard had the greatest anthocyanin (592.5 ± 86.4 mg/kg DW) and total phenolic (52.2 ± 3 mg GAE/g DW). It should be noted that the lowest anthocyanin and total phenolic content of shoots were found in P40, while tubers of P40 contain the highest content of each. Furthermore, the increase in leaf anthocyanin content over the growth stages that was observed in three of the cultivars but not in P40. No significant difference of anthocyanin content was found in Beauregard leaves grown in the high tunnels when compared with that in the open field. This study demonstrated for the first time that anthocyanin levels were significantly changed in response to various growth stages but not high tunnel condition, indicating that the effect of anthocyanin biosynthesis in sweet potato leaves is highly variable and genotype specific.

scholarly journals The Effects of Temperature and Potassium Fertilizer on the Growth, Yield, and Biochemical Parameters of Ipomoea batatas var. Antin-1

2020 ◽  
Vol 73 (3) ◽  
Author(s):  
Rini Sulistiani ◽  
Rosmayati ◽  
Luthfi A. M. Siregar ◽  
Fauziyah Harahap
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Growth Yield ◽  
Glucose Production ◽  
Morphological Properties ◽  
Anthocyanin Content ◽  
Potassium Fertilizer ◽  
Glucose Content ◽  
Four Levels ◽  
The Impact

The impact of temperature and potassium fertilizers on the growth, yield, and basic biochemical characteristics of sweet potato, <em>Ipomoea batatas </em>var. Antin- 1, was investigated in lowland and highland terrains. In this study we aimed to determine the different traits of the sweet potato, based on its morphology, physiology, and biochemical composition, following treatments with a potassium fertilizer at different temperatures. The study was undertaken using a nested factorial design. The first factor was temperature at each of the two sites: T1 (lowland) and T2 (highland). The second factor was potassium fertilizer (K<sub>2</sub>O) nested within the temperature factor and consisting of four levels of K: K0 (0 kg/ha), K1 (50 kg/ha), K2 (100 kg/ha), and K3 (150 kg/ha). Each potassium treatment was replicated three times at each location. Plant vegetative growth in the lowland region, with a daily average temperature of 26.8 °C, tended to be more bushy, with several different morphological properties: The main stem was longer (160.7 cm) and spreading, the stem diameter was greater, and the petioles were significantly longer in the lowland plants compared with the highland plants; however, no differences were observed in the leaf characteristics. The differential temperature (depending on altitude) resulted in significant differences in relative growth rates (RGR) and net assimilation rates (NAR); the values for RGR-1 and NAR-1 in the lowlands were higher than those in the highlands. The tuber weight, yield index, anthocyanin accumulation, and glucose content were significantly higher in the highland crops than in the lowland plants, which grew under relatively higher temperatures. Potassium applications caused significant differences in the anthocyanin content (mg/100 g fresh weight), anthocyanin production (kg/ha), and glucose production (kg/ha) at different locations.

scholarly journals Genetic analyses of anthocyanin content using polyploid GWAS followed by QTL detection in the sweetpotato (Ipomoea batatas L.) storage root

Plant Root ◽  
2020 ◽  
Vol 14 (0) ◽  
pp. 11-21
Author(s):  
Emdadul Haque ◽  
Eiji Yamamoto ◽  
Kenta Shirasawa ◽  
Hiroaki Tabuchi ◽  
Ung-Han Yoon ◽  
...  
Keyword(s):  
Ipomoea Batatas ◽  
Anthocyanin Content ◽  
Storage Root ◽  
Qtl Detection ◽  
Genetic Analyses

scholarly journals Effect of cutting position and vine pruning level on yield of Sweet Potato (Ipomoea Batatas L.)

1970 ◽  
pp. 01-05
Author(s):  
Ncube Netsai ◽  
Mutetwa Moses, Mtaita Tuarira
Keyword(s):  
Sweet Potato ◽  
Root Length ◽  
Ipomoea Batatas ◽  
Block Design ◽  
Root Diameter ◽  
Root Weight ◽  
Stem Cuttings ◽  
Storage Root ◽  
Storage Roots ◽  
Significant Difference

There is significant variation in yield of storage roots and vines of sweet potato (Ipomoea batatas) among farmers due to use of different cutting positions and pruning of vines at different levels. This study was carried out to establish the cutting position and the vine pruning level that give the best yield of both the storage roots and vines. The study was conducted in a 3x3 factorial arrangement in Randomized Complete Block Design (RCBD) with three replications. Treatments included cutting position at three levels (apical cutting, middle cutting and basal cutting) and pruning at three levels, 0%, 25% and 50% respectively. Pruning was done. 50 days after planting. And storage root harvesting was done 100 days after planting. The two measurements were summed up to give the total vine weight. Storage root length, diameter and weight were measured at 100 DAP. Storage root length indicated significant difference (P<0.05) only among cutting positions with highest mean length (16.20 cm) obtained from apical cutting and the lowest (11.98 cm) from basal cutting. Storage root diameter, storage root weight and vine weight indicated significant interaction (P<0.05) of cutting position and vine pruning level. Highest mean root diameter and root weight were obtained from middle cutting and 25% vine pruning level, with the lowest being obtained from basal cutting and 50% vine pruning level. Highest vine weight was recorded from middle cutting and 50% vine pruning level, with the lowest being recorded from basal cutting and 0% vine pruning level. Both middle and apical stem cuttings can be recommended for higher storage root and vine yield. Vine pruning at 25% can be adopted for higher storage root yield while pruning at 50% can be suggested for higher vine yield.

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