scholarly journals DNA marker for resistance to Puccinia horiana in chrysanthemum (Chrysanthemum morifolium Ramat.) “Southern Pegasus”

2021 ◽  
Author(s):  
Katsuhiko Sumitomo ◽  
Kenta Shirasawa ◽  
Sachiko N. Isobe ◽  
Hideki Hirakawa ◽  
Akiho Harata ◽  
...  
Keyword(s):  
Dna Marker ◽  
Chrysanthemum Morifolium ◽  
Puccinia Horiana

scholarly journals USE OF INTERSPECIFIC HYBRIDIZATION IN THE BREEDING OF ADAPTIVE HYBRIDS AND SORTS OF GARDEN CHRYSANTHEMUM (Chrysanthemum morifolium Ramat.)

2018 ◽  
Vol 22 (4) ◽  
pp. 476-483
Author(s):  
A. I. Nedoluzhko
Keyword(s):  
Interspecific Hybridization ◽  
Wild Species ◽  
Selection Process ◽  
Chrysanthemum Morifolium ◽  
Winter Hardiness ◽  
Local Conditions ◽  
The World ◽  
Research Objects ◽  
Chrysanthemum Morifolium Ramat ◽  
Puccinia Horiana

Many garden chrysanthemums bred across the world are not fully winter hardy. Many are damaged by fungal diseases due to a high humidity and are late flowering. This makes them unsuitable for general commercial marketing. Since 2000 we have been conducting a breeding program using natural species of the genus Chrysanthemum that are adapted to the local conditions. The strategy of breeding adaptive hybrids and varieties of chrysanthemum native to Russia was proposed based on their biological, genetic peculiarities and natural resources of Chrysanthemum with the use of interspecific hybridization. Research objects are the first generations of interspecific hyb rids of F1obtained previously by the author as a result of the hybridization of varieties and wild species of Chrysanthemum. Derived from different species and varieties, F1hybrids were crossed among themselves to obtain the multicomponent F2progeny. F2seedlings with winter hardiness, resistance to Puccinia horiana Henn. and early flowering were used in closely related crosses. The offspring of F3from closely related crosses were also assessed and selected according to adaptive and decorative characteristics. Inclusion in the selection process of various sources of winter hardiness and resistance to P. horiana allowed positive characteristics to be increased in F2and to be revealed in F3. Adaptive signs of the wild species Chrysanthemum naktongense Nakai, C. coreanum (H. Lév. et Vaniot) Nakai, C. zawadzkii var. tenuisectum Kitag., C. leiophyllum Nakai, and C. zawadzkii subsp. acutilobum (DC.) Kitag., which have formed and fixed during evolution, were inherited and manifested in offspring of the multicomponent hybrids and the closely related crosses. Promising interspecific forms with biological signs determining the possibility of growing in extreme conditions of the subregion were selected.

scholarly journals Morphology of Puccinia horiana, Causal Agent of Chrysanthemum White Rust, Sampled From Naturally Infected Plants

Plant Disease ◽  
2015 ◽  
Vol 99 (12) ◽  
pp. 1738-1743 ◽  
Author(s):  
G. O’Keefe ◽  
D. D. Davis
Keyword(s):  
Chrysanthemum Morifolium ◽  
Linear Pattern ◽  
White Rust ◽  
Internal Cell ◽  
High Infection ◽  
Commercially Important ◽  
Close Relatives ◽  
Puccinia Horiana ◽  
Germ Tubes

Chrysanthemum white rust (CWR), caused by Puccinia horiana, is pathogenic on many Chrysanthemum spp. and close relatives, and infects commercially important florist chrysanthemum cultivars (Chrysanthemum × morifolium) throughout the world. Due to regulations, most research and observations with CWR are done in vitro with symptomatic plants. In contrast, research presented herein is based on microscopic examination of symptomatic and asymptomatic plants collected from natural outbreaks in the field. We observed scattered (not in a linear pattern) telial sori on infected chrysanthemum leaves, stems, and flowers that coalesced at high infection levels. Teliospores were mainly two-celled but occasionally one- or three-celled. Promycelia arose from the apical teliospore cell, the basal cell, or both. The number of basidiospores on promycelia varied from one to four. Germ tubes, arising from P. horiana basidiospores, penetrated the host epidermis directly without appressoria. A mucilaginous exudate formed at the site of attachment and penetration of leaf and stem tissue, as well as on internal cell walls. P. horiana colonization was systemic, with intercellular mycelium and intracellular M-haustoria in both symptomatic and asymptomatic infected host tissue. Hyphal anastomosis was observed within infected plants, suggesting that asexual fusion between different P. horiana pathotypes or genotypes might occur.

Chemical control of Puccinia horiana in Chrysanthemum morifolium

1968 ◽  
Vol 74 (5) ◽  
pp. 170-173 ◽  
Author(s):  
R. Zandvoort ◽  
C. A. M. Groenewegen ◽  
J. C. Zadoks
Keyword(s):  
Chemical Control ◽  
Chrysanthemum Morifolium ◽  
Puccinia Horiana

scholarly journals PCR-Based Assays for the Detection of Puccinia horiana on Chrysanthemums

Plant Disease ◽  
2009 ◽  
Vol 93 (12) ◽  
pp. 1252-1258 ◽  
Author(s):  
Kerry F. Pedley
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Leaf Tissue ◽  
Chrysanthemum Morifolium ◽  
Nuclear Ribosomal Dna ◽  
Infected Leaf ◽  
White Rust ◽  
Pcr Assays ◽  
Species Specific ◽  
Puccinia Horiana

Puccinia horiana, the causal agent of chrysanthemum white rust, is a pathogen of quarantine status in many countries where Chrysanthemum × morifolium cultivars are grown. Historically, identification protocols for white rust relied upon macroscopic symptom development and microscopic examination of infected leaves for teliospores. Symptoms become visible 7 to 10 days after initial infection under favorable conditions followed by the production of telia. Infected plants can therefore evade detection before symptoms and fruiting bodies are evident. Conventional and real-time polymerase chain reaction (PCR) assays were developed to detect P. horiana using primers designed to amplify portions of the internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA (rDNA). The species-specific primers could detect the pathogen from 1 ng of DNA isolated from infected leaf tissue in conventional PCR assays and from 1 pg in real-time PCR assays. While both assays were capable of detecting P. horiana in symptomatic tissue, the greater sensitivity offered by the real-time PCR assay makes it more reliable for detecting the pathogen during the latent stage of infection. The P. horiana primers did not amplify the rDNA target using DNA isolated from leaf tissue infected with P. chrysanthemi.

Phylogenetic relationships of Puccinia horiana and other rust pathogens of Chrysanthemum×morifolium based on rDNA ITS sequence analysis

2009 ◽  
Vol 113 (6-7) ◽  
pp. 668-683 ◽  
Author(s):  
Hossein Alaei ◽  
Mathias De Backer ◽  
Jorinde Nuytinck ◽  
Martine Maes ◽  
Monica Höfte ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Phylogenetic Relationships ◽  
Chrysanthemum Morifolium ◽  
Its Sequence ◽  
Rdna Its ◽  
Its Sequence Analysis ◽  
Rust Pathogens ◽  
Puccinia Horiana

Methods for the inoculation of Chrysanthemum morifolium with Puccinia horiana

1968 ◽  
Vol 74 (5) ◽  
pp. 174-176 ◽  
Author(s):  
R. Zandvoort ◽  
C. A. M. Groenewegen ◽  
J. C. Zadoks
Keyword(s):  
Chrysanthemum Morifolium ◽  
Puccinia Horiana

DNA MARKER STUDY OF RCG1 LOCUS IN THE GRAPE GENOTYPES

2019 ◽  
Vol 23 ◽  
pp. 40-44
Author(s):  
E.T. Ilnitskaya ◽  
◽  
M.V. Makarkina ◽  
S.V. Tokmakov ◽  
◽  
...  
Keyword(s):  
Dna Marker ◽  
Marker Study
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