scholarly journals Identification of stress defensive proteins in common wheat-Thinopyron intermedium translocation line YW642 developing grains via comparative proteome analysis

2020 ◽  
Vol 70 (5) ◽  
pp. 517-529
Author(s):  
Yuxia Lu ◽  
Jisu Wu ◽  
Ruomei Wang ◽  
Yueming Yan
Keyword(s):  
Common Wheat ◽  
Proteome Analysis ◽  
Translocation Line ◽  
Developing Grains

A spontaneous translocation that transfers wheat curl mite resistance from decaploid Agropyron elongatum to common wheat

Genome ◽  
1988 ◽  
Vol 30 (3) ◽  
pp. 289-292 ◽  
Author(s):  
E. D. P. Whelan ◽  
G. E. Hart
Keyword(s):  
Common Wheat ◽  
Dominant Gene ◽  
Triticum Aestivum L ◽  
Translocation Line ◽  
Structural Genes ◽  
Agropyron Elongatum ◽  
Selfed Progeny ◽  
Wheat Curl Mite ◽  
Group 6 ◽  
Homoeologous Chromosomes

The wheat curl mite (Eriophyes tulipae Keifer) is the vector of wheat streak mosaic virus, a damaging disease of winter wheat. A translocation between a common wheat (Triticum aestivum L.) chromosome and a group 6 chromosome (6Ag) from decaploid Agropyron elongatum (Host) Beauv. resulted in transfer of resistance to colonization by the wheat curl mite. Transmission of resistance through the pollen and the egg were similar and not significantly different from 50%. The frequency of resistance in the F2 generation (65.6%) was lower than expected for a single, dominant gene. In the F2, 26.7% of the resistant plants were homozygous for resistance. Selfed progeny from monosomic and disomic F1 plants from crosses between the translocation line and monosomics for 6A and 6B segregated with frequencies similar to normal F2 progeny but the progeny of monosomics for 6D were primarily resistant (93.2%). Crosses between the translocation line and chromosome 6D telocentrics and studies of four enzymes that are encoded by genes on the group 6 homoeologous chromosomes showed that the translocated chromosome consists of the q arm of chromosome 6D of 'Rescue' and the p arm of chromosome 6 of A. elongatum. Because the new stock was derived from a double monosomic, the translocation was probably a Robertsonian fusion of misdivided centromeres. The resistance is being backcrossed into winter wheat.Key words: Agropyron elongatum, Thinopyron, Elytrigia, Lophopyrum, Robertsonian translocation, isozyme structural genes, wheat curl mite.

scholarly journals New Insights Into the Introgression Between Agropyron Cristatum P Genome and Wheat Genome

2021 ◽  
Author(s):  
Zhi Zhang ◽  
Shenghui Zhou ◽  
Weihua Liu ◽  
Liqiang Song ◽  
Jinpeng Zhang ◽  
...  
Keyword(s):  
Disease Resistance ◽  
In Situ Hybridization ◽  
Common Wheat ◽  
Agronomic Traits ◽  
Wheat Genome ◽  
Agropyron Cristatum ◽  
Translocation Line ◽  
Gene Markers ◽  
Physiological Races

Abstract Agropyron cristatum (2n = 4x = 28, PPPP) is an important wild relative of common wheat and confers desirable agronomic traits to common wheat. A previous report showed that the wheat-A. cristatum 6P translocation line WAT655 carrying A. cristatum 6PS (0.81–1.00) exhibited high resistance to prevalent physiological races (CYR32 and CYR33). In this study, three disease resistance-related transcriptomes, which were mapped to A. cristatum 6PS (0.81–1.00) through the analysis of specific molecular markers, were searched from among A. cristatum full-length transcriptomes. Then, three disease resistance-related gene markers, A. cristatum P genome-specific markers, and fluorescence in situ hybridization (FISH)/genomic in situ hybridization (GISH) probes made from the DNA of three bacterial artificial chromosome (BAC) clones, three genes, and A. cristatum “Z559” were used to analyze the BC5F2 and BC5F2:3 genetic populations of the translocation line WAT655. The results revealed the introgression can spontaneously occur between A. cristatum P genome and wheat genome, and indicated the three genes could constitute a gene cluster according to the positions of their FISH signals. Additionally, kompetitive allele-specific PCR (KASP) markers of the three genes were developed to detect and acquire 24 wheat-A. cristatum breeding materials, which showed resistance to physiological races (CYR32 and CYR33) and other desirable agronomic traits according to the field investigation. In conclusion, our study not only provides new insights into the introgression between A. cristatum P genome and wheat genome, but also provides the desirable breeding materials for breeding practice.

scholarly journals Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit

2018 ◽  
Vol 9 ◽  
Author(s):  
Xiong Deng ◽  
Yue Liu ◽  
Xuexin Xu ◽  
Dongmiao Liu ◽  
Genrui Zhu ◽  
...  
Keyword(s):  
Water Deficit ◽  
Proteome Analysis ◽  
Flag Leaves ◽  
Developing Grains

scholarly journals Small RNA and Degradome Analyses Uncovering the Extensive Effects of miRNAs and Targets in Early Developing Grains of Common Wheat

2020 ◽  
Author(s):  
Qiaoyan Chen ◽  
Lina Xu ◽  
Yuanyuan Guan ◽  
Zeeshan Ali Buttar ◽  
Gan Li ◽  
...  
Keyword(s):  
Common Wheat ◽  
Small Rna ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Wheat Breeding ◽  
Differentially Expressed ◽  
Grain Development ◽  
Wheat Grain ◽  
Small Regulatory Rnas ◽  
Developing Grains

Abstract Background: The development of grains is important for wheat production, and wheat (Triticum aestivum) is one of the staple food crops worldwide. MicroRNAs (miRNAs), as a kind of small regulatory RNAs, play important roles during plant growth and development. Although the development of plant grain/seed is widely researched, there is limited knowledge on miRNAs’s regulation of early developing wheat grains. Results: In the present study, miRNAs and their targets were explored in early developing grains of wheat cultivar “Bainong 4199” at 7DAP and 14 DAP using high-throughput small RNA and degradome sequencing. A total of 105 known and 79 novel miRNAs were identified, including 46 known and 32 novel miRNAs from 7 DAP library and 87 known and 78 novel miRNAs from 14 DAP library, respectively. Expression analysis of miRNAs revealed that 39 miRNAs including 19 known and 20 novel miRNAs were differentially expressed between 7 DAP and 14 DAP. In total, 266 targets for 40 known wheat miRNAs, 152 targets for 13 other known plant miRNAs and 258 targets for 25 novel miRNAs were predicted across small RNA and degradome analyses. For differentially expressed miRNAs, 23 targets were predicted to be cleaved by 7 miRNAs, including 3 known and 4 novel miRNAs. Majority of the miRNAs potentially regulated multiple targets, whereas some miRNAs only acted on a single target gene. Functional analyses showed that miRNAs and their targets widely participated in the regulations of early wheat grain development and metabolism. The expression patterns of the randomly selected miRNAs and targets were validated using quantitative real-time polymerase chain reaction, and showed consistent and reliable results. Conclusion: This study suggests that quite a few known and novel miRNAs and their targets play extensive roles during the early grain development of common wheat. Understanding of miRNA-mediated regulatory network involved in wheat grain development will help us to elucidate the molecular mechanisms underlying wheat grain development and carry out ingenious molecular improvements in wheat breeding.

scholarly journals Development of Novel Wheat-Rye Chromosome 4R Translocations and Assignment of Their Powdery Mildew Resistance

Plant Disease ◽  
2020 ◽  
Vol 104 (1) ◽  
pp. 260-268 ◽  
Author(s):  
Pengtao Ma ◽  
Guohao Han ◽  
Qi Zheng ◽  
Shiyu Liu ◽  
Fangpu Han ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Common Wheat ◽  
Powdery Mildew Resistance ◽  
Translocation Line ◽  
Growth Stages ◽  
Alien Chromosome ◽  
Mildew Resistance ◽  
Wheat Improvement ◽  
Chromosome Arm

Rye (Secale cereale L.) is an important gene donor for wheat improvement because of its many valuable traits, especially disease resistance. Development of novel wheat-rye translocations with disease resistance can contribute to transferring resistance into common wheat. In a previous study, a wheat-rye T4BL·4RL and T7AS·4RS translocation line (WR41-1) was developed by distant hybridization, and it was speculated that its resistance to powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), was derived from rye based on pedigree analysis. To make accurate use of chromosome 4R in wheat improvement, a set of new 4R translocations involving different arm translocations (e.g., 4RS monosomic, 4RL monosomic, 4RL disomic, 4RS monosomic plus 4RL monosomic, 4RS monosomic plus 4RL disomic, and 4RS disomic plus 4RL disomic translocations) was developed from crosses with common wheat. Those translocations were characterized by genomic in situ hybridization and expressed sequence tag simple sequence repeat marker analysis. To confirm the source of powdery mildew resistance, the translocation plants were tested against Bgt isolate E09. The results indicated that all translocations with 4RL were resistant at all tested growth stages, whereas those with only 4RS translocation or no alien translocation were susceptible. This further indicated that the powdery mildew resistance of WR41-1 was derived from the alien chromosome arm 4RL. To effectively use 4RL resistance in wheat improvement, two competitive allele-specific PCR markers specific for chromosome arm 4RL were developed to detect the alien chromosome in the wheat genome. These new translocation lines with diagnostic markers can efficiently serve as important bridges for wheat improvement.

scholarly journals Cytogenetic identification and molecular marker development for the novel stripe rust-resistant wheat–Thinopyrum intermedium translocation line WTT11

aBIOTECH ◽  
2021 ◽  
Author(s):  
Guotang Yang ◽  
Qi Zheng ◽  
Pan Hu ◽  
Hongwei Li ◽  
Qiaoling Luo ◽  
...  
Keyword(s):  
Common Wheat ◽  
Stripe Rust ◽  
Agronomic Traits ◽  
Puccinia Striiformis ◽  
Snp Array ◽  
Stripe Rust Resistance ◽  
Translocation Line ◽  
Resistance Locus ◽  
Thinopyrum Intermedium ◽  
Wheat Group

AbstractStripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. Xiaoyan 78829, a partial amphidiploid developed by crossing common wheat with Thinopyrum intermedium, is immune to wheat stripe rust. To transfer the resistance gene of this excellent germplasm resource to wheat, the translocation line WTT11 was produced by pollen irradiation and assessed for immunity to stripe rust races CYR32, CYR33 and CYR34. A novel stripe rust-resistance locus derived from Th. intermedium was confirmed by linkage and diagnostic marker analyses. Molecular cytogenetic analyses revealed that WTT11 carries a TTh·2DL translocation. The breakpoint of 1B was located at 95.5 MB, and the alien segments were found to be homoeologous to wheat-group chromosomes 6 and 7 according to a wheat660K single-nucleotide polymorphism (SNP) array analysis. Ten previously developed PCR-based markers were confirmed to rapidly trace the alien segments of WTT11, and 20 kompetitive allele-specific PCR (KASP) markers were developed to enable genotyping of Th. intermedium and common wheat. Evaluation of agronomic traits in two consecutive crop seasons uncovered some favorable agronomic traits in WTT11, such as lower plant height and longer main panicles, that may be applicable to wheat improvement. As a novel genetic resource, the new resistance locus may be useful for wheat disease-resistance breeding.

Proteome analysis of cerebrospinal fluid in patients with Multiple System Atrophy (MSA)

2008 ◽  
Vol 35 (S 01) ◽  
Author(s):  
S Werz ◽  
V Lehmensiek ◽  
S Süssmuth ◽  
H Mogel ◽  
J Brettschneider ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Multiple System Atrophy ◽  
Proteome Analysis
Sign in / Sign up

Export Citation Format

Share Document