scholarly journals Allele specific DNA marker for fusarium resistance gene FocBo1 in Brassica oleracea

2019 ◽  
Vol 69 (2) ◽  
pp. 308-315
Author(s):  
Maho Sato ◽  
Motoki Shimizu ◽  
Daniel J. Shea ◽  
Mozammel Hoque ◽  
Takahiro Kawanabe ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Resistance Gene ◽  
Dna Marker ◽  
Fusarium Resistance ◽  
Allele Specific

scholarly journals Two User-Friendly Molecular Markers Developed for the Identification of Hybrid Lethality Genes in Brassica oleracea

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 982
Author(s):  
Zhiliang Xiao ◽  
Congcong Kong ◽  
Fengqing Han ◽  
Limei Yang ◽  
Mu Zhuang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Oleracea ◽  
Rapid Identification ◽  
Inbred Lines ◽  
Hybrid Lethality ◽  
Specific Pcr ◽  
Allele Specific ◽  
User Friendly ◽  
Allele Specific Pcr ◽  
Kasp Marker

Cabbage (Brassica oleracea) is an important vegetable crop that is cultivated worldwide. Previously, we reported the identification of two dominant complementary hybrid lethality (HL) genes in cabbage that could result in the death of hybrids. To avoid such losses in the breeding process, we attempted to develop molecular markers to identify HL lines. Among 54 previous mapping markers closely linked to BoHL1 or BoHL2, only six markers for BoHL2 were available in eight cabbage lines (two BoHL1 lines; three BoHL2 lines; three lines without BoHL); however, they were neither universal nor user-friendly in more inbred lines. To develop more accurate markers, these cabbage lines were resequenced at an ~20× depth to obtain more nucleotide variations in the mapping regions. Then, an InDel in BoHL1 and a single-nucleotide polymorphism (SNP) in BoHL2 were identified, and the corresponding InDel marker MBoHL1 and the competitive allele-specific PCR (KASP) marker KBoHL2 were developed and showed 100% accuracy in eight inbred lines. Moreover, we identified 138 cabbage lines using the two markers, among which one inbred line carried BoHL1 and 11 inbred lines carried BoHL2. All of the lethal line genotypes obtained with the two markers matched the phenotype. Two markers were highly reliable for the rapid identification of HL genes in cabbage.

scholarly journals Detection of Blackleg Resistance Gene Rlm1 in Double-Low Rapeseed Accessions from Sichuan Province, by Kompetitive Allele-Specific PCR

2021 ◽  
Vol 37 (2) ◽  
pp. 194-199
Author(s):  
Liang Chai ◽  
Jinfang Zhang ◽  
Wannakuwattewaduge Gerard Dilantha Fernando ◽  
Haojie Li ◽  
Xiaoqin Huang ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Sichuan Province ◽  
Blackleg Resistance ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

scholarly journals Fine mapping of Brassica napus blackleg resistance gene Rlm1 through bulked segregant RNA sequencing

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Fuyou Fu ◽  
Xunjia Liu ◽  
Rui Wang ◽  
Chun Zhai ◽  
Gary Peng ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Resistance Gene ◽  
Rna Sequencing ◽  
Catalytic Domain ◽  
Leptosphaeria Maculans ◽  
Snp Markers ◽  
Specific Pcr ◽  
Dual Specificity ◽  
Kinase Catalytic Domain ◽  
Allele Specific

Abstract The fungal pathogen Leptosphaeria maculans causes blackleg disease on canola and rapeseed (Brassica napus) in many parts of the world. A B. napus cultivar, ‘Quinta’, has been widely used for the classification of L. maculans into pathogenicity groups. In this study, we confirmed the presence of Rlm1 in a DH line (DH24288) derived from B. napus cultivar ‘Quinta’. Rlm1 was located on chromosome A07, between 13.07 to 22.11 Mb, using a BC1 population made from crosses of F1 plants of DH16516 (a susceptible line) x DH24288 with bulked segregant RNA Sequencing (BSR-Seq). Rlm1 was further fine mapped in a 100 kb region from 19.92 to 20.03 Mb in the BC1 population consisting of 1247 plants and a F2 population consisting of 3000 plants using SNP markers identified from BSR-Seq through Kompetitive Allele-Specific PCR (KASP). A potential resistance gene, BnA07G27460D, was identified in this Rlm1 region. BnA07G27460D encodes a serine/threonine dual specificity protein kinase, catalytic domain and is homologous to STN7 in predicted genes of B. rapa and B. oleracea, and A. thaliana. Robust SNP markers associated with Rlm1 were developed, which can assist in introgression of Rlm1 and confirm the presence of Rlm1 gene in canola breeding programs.

Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea

2016 ◽  
Vol 129 (8) ◽  
pp. 1625-1637 ◽  
Author(s):  
Hai-long Yu ◽  
Zhi-yuan Fang ◽  
Yu-mei Liu ◽  
Li-mei Yang ◽  
Mu Zhuang ◽  
...  
Keyword(s):  
Brassica Oleracea ◽  
Interspecific Hybrids ◽  
Allele Specific ◽  
Novel Allele

scholarly journals Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering

Genetics ◽  
2011 ◽  
Vol 187 (4) ◽  
pp. 1011-1021 ◽  
Author(s):  
Zhixia Niu ◽  
Daryl L. Klindworth ◽  
Timothy L. Friesen ◽  
Shiaoman Chao ◽  
Yue Jin ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stem Rust ◽  
Rust Resistance ◽  
Dna Marker ◽  
Stem Rust Resistance ◽  
Rust Resistance Gene ◽  
Stem Rust Resistance Gene ◽  
Chromosome Engineering ◽  
Wheat Stem Rust

scholarly journals Expression of the Fusarium resistance gene I-2 colocalizes with the site of fungal containment

2000 ◽  
Vol 23 (2) ◽  
pp. 183-193 ◽  
Author(s):  
Jurriaan J. Mes ◽  
Aveline A. Van Doorn ◽  
Jelle Wijbrandi ◽  
Guus Simons ◽  
Ben J. C. Cornelissen ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Fusarium Resistance
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