scholarly journals Genome-wide characterization of microsatellites and genetic diversity assessment of spinach in the Chinese germplasm collection

2018 ◽  
Vol 68 (4) ◽  
pp. 455-464 ◽  
Author(s):  
Shu-Fen Li ◽  
Bing-Xiao Wang ◽  
Yu-Jiao Guo ◽  
Chuan-Liang Deng ◽  
Wu-Jun Gao
2011 ◽  
Vol 68 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Patrícia Coelho de Souza Leão ◽  
Cosme Damião Cruz ◽  
Sérgio Yoshimitsu Motoike

The conservation and characterization of grape (Vitis spp) genetic resources in germplasm banks have been the basis of its use in breeding programs that result in development of new cultivars. There are at least 10,000 grape cultivars kept in germplasm collection. The genetic diversity in 136 table grape accessions from the state of Bahia, Brazil, was evaluated. Continuous and discrete morphoagronomic traits were assessed. The clustering analysis by the Tocher otimization method resulted in 30 clusters (considering continuous morphoagronomic traits), and 9 clusters (taking into consideration multicategorical traits). There was no agreement between clusters obtained by both, continuous or discrete phenotypic descriptors, independent of the cluster method analysis used. A satisfactory genetic variability among the table grape accessions was observed.


2021 ◽  
pp. 1-10
Author(s):  
Veluru Bhargav ◽  
Rajiv Kumar ◽  
Anuradha Sane ◽  
T. Manjunatha Rao ◽  
T. Usha Bharathi ◽  
...  

Abstract Understanding genetic diversity in target populations is of great importance in breeding and a prerequisite for association mapping of traits. In this study, 57 cross species simple sequence repeat (SSR) markers were screened for amplification in China aster. Twenty six polymorphic markers were used to estimate the genetic diversity in forty two China aster genotypes. The observed and expected heterozygosities within the genotypes were ranged from 0.00 to 0.80 and 0.17 to 0.50, respectively. Weighted Neighbor Joining method, grouped China aster genotypes into five major clusters which coincided for morphological traits mostly flower color and form, but not correlated for their geographical locations. The results suggested that, population may be useful for the genome-wide marker–trait association mapping. These set of cross species transferable SSR markers would enable the application of the SSR technique in China aster crop improvement.


2017 ◽  
Vol 37 (5) ◽  
Author(s):  
Honglin Chen ◽  
Liping Liu ◽  
Lixia Wang ◽  
Suhua Wang ◽  
Ming Li Wang ◽  
...  

2018 ◽  
Vol 69 (2) ◽  
pp. 73-83 ◽  
Author(s):  
Gábor Mészáros

Summary The characterization of livestock genetic diversity has experienced extensive changes with the availability of dense nucleotide markers. Among the various forms of markers, the single nucleotide polymorphisms (SNP) have arguably the largest influence. A wide range of indicators for the assessment of genetic diversity was developed, or the existing methods were improved, enabling us to make informed decisions on the management of livestock populations. This review discusses the selected aspects of diversity assessment, with special attention to the SNP based methods. One of the core concepts in genomics of diversity is the linkage disequilibrium (LD), as it was shaped by demographic events during the development of breeds and species. These events, either natural or artificial, left detectable signals within the livestock genomes. Further changes were induced by human activity when mating related animals, leading to fixing or improving the desired traits in the breed, but reducing their genetic variability. The assessment of relatedness is also pivotal to construct meaningful mating plans and to avoid the negative consequences of inbreeding depression that might be detrimental especially in small, endangered populations. Both LD and relatedness are of interest on their own, as well as in their follow-up applications deriving overall measures of effective population size.


2021 ◽  
Vol 12 ◽  
Author(s):  
Aamir Saleem ◽  
Hilde Muylle ◽  
Jonas Aper ◽  
Tom Ruttink ◽  
Jiao Wang ◽  
...  

Targeted and untargeted selections including domestication and breeding efforts can reduce genetic diversity in breeding germplasm and create selective sweeps in crop genomes. The genomic regions at which selective sweeps are detected can reveal important information about signatures of selection. We have analyzed the genetic diversity within a soybean germplasm collection relevant for breeding in Europe (the EUCLEG collection), and have identified selective sweeps through a genome-wide scan comparing that collection to Chinese soybean collections. This work involved genotyping of 480 EUCLEG soybean accessions, including 210 improved varieties, 216 breeding lines and 54 landraces using the 355K SoySNP microarray. SNP calling of 477 EUCLEG accessions together with 328 Chinese soybean accessions identified 224,993 high-quality SNP markers. Population structure analysis revealed a clear differentiation between the EUCLEG collection and the Chinese materials. Further, the EUCLEG collection was sub-structured into five subgroups that were differentiated by geographical origin. No clear association between subgroups and maturity group was detected. The genetic diversity was lower in the EUCLEG collection compared to the Chinese collections. Selective sweep analysis revealed 23 selective sweep regions distributed over 12 chromosomes. Co-localization of these selective sweep regions with previously reported QTLs and genes revealed that various signatures of selection in the EUCLEG collection may be related to domestication and improvement traits including seed protein and oil content, phenology, nitrogen fixation, yield components, diseases resistance and quality. No signatures of selection related to stem determinacy were detected. In addition, absence of signatures of selection for a substantial number of QTLs related to yield, protein content, oil content and phenological traits suggests the presence of substantial genetic diversity in the EUCLEG collection. Taken together, the results obtained demonstrate that the available genetic diversity in the EUCLEG collection can be further exploited for research and breeding purposes. However, incorporation of exotic material can be considered to broaden its genetic base.


Author(s):  
K. Abirami ◽  
V. Baskaran ◽  
P. Simhachalam

Inter-simple sequence repeat (ISSR) analysis was used to study the genetic diversity of Piper spp. with its relative genus Peperomia pellucida. Among the 38 ISSR primers used, 10 primers generated 1083 bands, of which seven (80.8%) were polymorphic. The maximum polymorphism were obtained from the primers UBC 881, UBC 889, UBC 848 and UBC 830. Cluster analysis grouped the species studied into two major clusters. One cluster comprised the different accessions of Piper nigrum and is distinct from the other species of Piperaceae. The other cluster grouped the four species of Piperaceae namely Piper longum, Piper sarmentosum, Piper betle and Peperomia pellucida. The variability studies also revealed that the ISSR marker could group the accessions within the same species based on their similar geographical origin. Assessment of genetic diversity among the different species of the family Piperaceae through ISSR marker would help in rapid identification of polymorphism, assist in future germplasm collection, conservation and domestication programmes. 


2020 ◽  
Vol 33 (2) ◽  
pp. 571-578
Author(s):  
MARILHA VIEIRA DE BRITO ◽  
VERÔNICA BRITO DA SILVA ◽  
CARLOS HUMBERTO AIRES MATOS FILHO ◽  
REGINA LUCIA FERREIRA-GOMES ◽  
ÂNGELA CELIS DE ALMEIDA LOPES

ABSTRACT To estimate the genetic diversity among the genotypes of a germplasm collection, a combined analysis of qualitative and quantitative variables was performed. The objective was to promote the morpho-agronomic characterization and estimate the genetic diversity of lima bean genotypes belonging to the Phaseolus Germplasm Bank of UFPI through univariate and multivariate approaches. The experiment was conducted on a screen house between January and September 2016, using a completely randomized design with four replications, with a plot consisting of a pot with a plant. An analysis of variance of the quantitative characteristics was performed and used to estimate the comparison of means. The combined analysis of the quantitative and qualitative variables was performed based on the Gower distance. Subsequently, the genotypes were grouped by the UPGMA method, from which five groups were formed. The lima bean genotypes showed wide genetic variability in relation to morpho-agronomic characteristics.


Sign in / Sign up

Export Citation Format

Share Document