scholarly journals Neutral alleles at hybrid sterility loci of Oryza glaberrima from AA genome relatives in Genus Oryza

2018 ◽  
Vol 68 (3) ◽  
pp. 343-351 ◽  
Author(s):  
Jing Li ◽  
Jiawu Zhou ◽  
Peng Xu ◽  
Xianneng Deng ◽  
Wei Deng ◽  
...  
Keyword(s):  
Hybrid Sterility ◽  
Oryza Glaberrima ◽  
Aa Genome

Genetic dissection of a chromosomal region conferring hybrid sterility using multi-donors from Oryza glaberrima

Euphytica ◽  
2010 ◽  
Vol 175 (3) ◽  
pp. 395-407 ◽  
Author(s):  
Jiawu Zhou ◽  
Peng Xu ◽  
Xianneng Deng ◽  
Jing Li ◽  
Fengyi Hu ◽  
...  
Keyword(s):  
Chromosomal Region ◽  
Hybrid Sterility ◽  
Oryza Glaberrima ◽  
Genetic Dissection

scholarly journals The role of S1-g allele from Oryza glaberrima in improving interspecific hybrid sterility between O. sativa and O. glaberrima

2010 ◽  
Vol 60 (4) ◽  
pp. 342-346 ◽  
Author(s):  
Xianneng Deng ◽  
Jiawu Zhou ◽  
Peng Xu ◽  
Jing Li ◽  
Fengyi Hu ◽  
...  
Keyword(s):  
Interspecific Hybrid ◽  
Hybrid Sterility ◽  
Oryza Glaberrima

scholarly journals The genic nature of gamete eliminator in rice.

Genetics ◽  
1990 ◽  
Vol 125 (1) ◽  
pp. 183-191 ◽  
Author(s):  
Y Sano
Keyword(s):  
Genetic Basis ◽  
Hybrid Sterility ◽  
Oryza Glaberrima ◽  
Male Sterile ◽  
Cultivated Rice ◽  
Sterility Gene ◽  
Wx Locus ◽  
Allelic Interaction ◽  
Different Genetic Background ◽  
Marked Suppression

Abstract The two cultivated rice species, Oryza sativa and Oryza glaberrima, are morphologically alike but are reproductively isolated from each other by hybrid sterility. The hybrid is male sterile but partially female fertile. Backcross experiments were conducted to introduce an alien factor controlling hybrid sterility from O. glaberrima (W025) into O. sativa (T65wx) and examine the genetic basis. An extracted sterility factor, closely linked to the wx locus, induced gametic abortion due to allelic interaction and was tentatively designated as S(t). The segregation patterns for infertility was explained by assuming that W025 and T65wx carried S(t) and S(t)a, respectively, and gametes with S(t)a aborted only in the heterozygote (S(t)/S(t)a) although the elimination of female gametes was incomplete. Thus, S(t) seemed to be intermediate between a gamete eliminator and pollen killer. However, S(t) was proven to be likely the same as S1 which was formerly reported as gamete eliminator in a different genetic background of O. sativa. In addition, a chromosomal segment containing S1 (or S(t] caused a marked suppression of crossing over around it, suggesting the presence of an inversion. Further, female transmission of S1a increased as the segment containing S1 became small by recombination. After S1 was further purified by successive backcrosses up to the BC15 generation, it became pollen killer. The present results give evidence that a profound sterility gene such as gamete eliminator can be made from accumulation of pollen killer and its modifier(s) when pollen killer and modifier(s) are linked, they behave as a gene complex in the hybrid.

scholarly journals Genetic Mapping of the Gamete Eliminator Locus, S2, Causing Hybrid Sterility and Transmission Ratio Distortion Found between Oryza sativa and Oryza glaberrima Cross Combination

Agriculture ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 268
Author(s):  
Myint Zin Mar ◽  
Yohei Koide ◽  
Mei Ogata ◽  
Daichi Kuniyoshi ◽  
Yoshiki Tokuyama ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Molecular Basis ◽  
Hybrid Sterility ◽  
Transmission Ratio Distortion ◽  
Oryza Glaberrima ◽  
Isogenic Line ◽  
Near Isogenic Line ◽  
Cross Combination ◽  
Asian Rice ◽  
Ratio Distortion

Hybrid sterility is a reproductive barrier that prevents gene flow between species. In Oryza species, some hybrid sterility loci, which are classified as gamete eliminators, cause pollen and seed sterility and sex-independent transmission ratio distortion (siTRD) in hybrids. However, the molecular basis of siTRD has not been fully characterized because of lacking information on causative genes. Here, we analyze one of the hybrid sterility loci, S2, which was reported more than forty years ago but has not been located on rice chromosomes. Hybrids between African rice (Oryza glaberrima) and a near-isogenic line that possesses introgressed chromosomal segments from Asian rice (Oryza sativa) showed sterility and siTRD, which confirms the presence of the S2 locus. Genome-wide SNP marker survey revealed that the near-isogenic line has an introgression on chromosome 4. Further substitution mapping located the S2 locus between 22.60 Mb and 23.54 Mb on this chromosome. Significant TRD in this chromosomal region was also observed in a calli population derived from cultured anther in hybrids of another cross combination of African and Asian rice species. This indicates that the pollen abortion caused by the S2 locus occurs before callus induction in anther culture. It also suggests the wide existence of the S2-mediated siTRD in this interspecific cross combination. Chromosomal location of the S2 locus will be valuable for identifying causative genes and for understanding of the molecular basis of siTRD.

scholarly journals Lineage-specific gene acquisition or loss is involved in interspecific hybrid sterility in rice

2018 ◽  
Vol 115 (9) ◽  
pp. E1955-E1962 ◽  
Author(s):  
Yohei Koide ◽  
Atsushi Ogino ◽  
Takanori Yoshikawa ◽  
Yuki Kitashima ◽  
Nozomi Saito ◽  
...  
Keyword(s):  
Evolutionary Biology ◽  
Single Gene ◽  
Hybrid Sterility ◽  
Reproductive Barrier ◽  
Specific Gene ◽  
Oryza Glaberrima ◽  
Breeding Programs ◽  
Gene Acquisition ◽  
Hybrid Plants ◽  
Functional Phenotype

Understanding the genetic basis of reproductive barriers between species has been a central issue in evolutionary biology. The S1 locus in rice causes hybrid sterility and is a major reproductive barrier between two rice species, Oryza sativa and Oryza glaberrima. The O. glaberrima-derived allele (denoted S1g) on the S1 locus causes preferential abortion of gametes with its allelic alternative (denoted S1s) in S1g/S1s heterozygotes. Here, we used mutagenesis and screening of fertile hybrid plants to isolate a mutant with an allele, S1mut, which does not confer sterility in the S1mut/S1g and S1mut/S1s hybrids. We found that the causal mutation of the S1mut allele was a deletion in the peptidase-coding gene (denoted “SSP”) in the S1 locus of O. glaberrima. No orthologous genes of SSP were found in the O. sativa genome. Transformation experiments indicated that the introduction of SSP in carriers of the S1s allele did not induce sterility. In S1mut/S1s heterozygotes, the insertion of SSP led to sterility, suggesting that SSP complemented the loss of the functional phenotype of the mutant and that multiple factors are involved in the phenomenon. The polymorphisms caused by the lineage-specific acquisition or loss of the SSP gene were implicated in the generation of hybrid sterility. Our results demonstrated that artificial disruption of a single gene for the reproductive barrier creates a “neutral” allele, which facilitates interspecific hybridization for breeding programs.

scholarly journals A Hypersensitivity-Like Response to Meloidogyne graminicola in Rice (Oryza sativa)

2018 ◽  
Vol 108 (4) ◽  
pp. 521-528 ◽  
Author(s):  
Ngan Thi Phan ◽  
Dirk De Waele ◽  
Mathias Lorieux ◽  
Lizhong Xiong ◽  
Stephane Bellafiore
Keyword(s):  
Resistance Genes ◽  
Defense Mechanism ◽  
Hybrid Sterility ◽  
Infection Process ◽  
Oryza Glaberrima ◽  
Sources Of Resistance ◽  
Major Step ◽  
Root Cells ◽  
Meloidogyne Graminicola

Meloidogyne graminicola is a major plant-parasitic nematode affecting rice cultivation in Asia. Resistance to this nematode was found in the African rice genotypes Oryza glaberrima and O. longistaminata; however, due to interspecific hybrid sterility, the introgression of resistance genes in the widely consumed O. sativa varieties remains challenging. Recently, resistance was found in O. sativa and, here, we report for the first time the histological and genetic characterization of the resistance to M. graminicola in Zhonghua 11, an O. sativa variety. Bright-light microscopy and fluorescence observations of the root tissue of this variety revealed that the root cells surrounding the nematode displayed a hypersensitivity-like reaction with necrotic cells at early stages of infection when nematodes are migrating in the root’s mesoderm. An accumulation of presumably phenolic compounds in the nematodes’ neighboring root cells was also observed. In addition, at a later stage of infection, not only were few feeding sites observed but also the giant cells were underdeveloped, underlining an incompatible interaction. Furthermore, we generated a hybrid O. sativa population by crossing Zhonghua 11 with the susceptible O. sativa variety IR64 in order to describe the genetic background of this resistance. Our data suggested that the resistance to M. graminicola infection was qualitative rather than quantitative and, therefore, major resistance genes must be involved in this infection process. The full characterization of the defense mechanism and the preliminary study of the genetic inheritance of novel sources of resistance to Meloidogyne spp. in rice constitute a major step toward their use in crop breeding.

Identification of four genes for stable hybrid sterility and an epistatic QTL from a cross between Oryza sativa and Oryza glaberrima

Euphytica ◽  
2008 ◽  
Vol 164 (3) ◽  
pp. 699-708 ◽  
Author(s):  
Jing Li ◽  
Peng Xu ◽  
Xianneng Deng ◽  
Jiawu Zhou ◽  
Fengyi Hu ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Hybrid Sterility ◽  
Oryza Glaberrima ◽  
Epistatic Qtl ◽  
Stable Hybrid

Application of indica–japonica single-nucleotide polymorphism markers for diversity analysis of Oryza AA genome species

2014 ◽  
Vol 12 (S1) ◽  
pp. S36-S40 ◽  
Author(s):  
Yoo-Jin Lee ◽  
Michael J. Thomson ◽  
Joong Hyoun Chin
Keyword(s):  
Genetic Relationships ◽  
Snp Markers ◽  
Oryza Rufipogon ◽  
Phylogenetic Study ◽  
Oryza Glaberrima ◽  
Nucleotide Polymorphisms ◽  
Cultivated Rice ◽  
Allele Polymorphism ◽  
Single Nucleotide ◽  
Aa Genome

High-throughput genotyping using single-nucleotide polymorphisms (SNP) is one tool that can be used to study the genetic relationships between wild rice relatives and cultivated rice. In this study, a set of 96 indica–japonica SNP markers, which can differentiate indica and japonica subspecies of rice, were used to characterize 227 Oryza accessions including 93 AA genome accessions from seven wild Oryza species. A total of 72 markers of the 96 markers were selected for the phylogenetic study and allele polymorphism survey. A subset of SNP markers were present only in Oryza sativa and evolutionarily close species, Oryza nivara and Oryza rufipogon. These markers can be used for distinguishing cultivated rice from the other species and vice versa. Eight clusters were generated through phylogenetic analysis, and Oryza meridionalis and Oryza longistaminata appeared to be the most distantly related species to cultivated rice. In this study, Oryza barthii and Oryza glaberrima accessions were found to exhibit high genetic similarity. Across the wild species, more indica-type alleles were detected for most accessions. In this study, a set of markers selected to be informative across O. sativa accessions were used, but it will be interesting to compare the results of this study with SNP data obtained through next-generation sequencing in the future.

scholarly journals Mapping QTLs for hybrid sterility in three AA genome wild species of Oryza

2016 ◽  
Vol 66 (3) ◽  
pp. 367-371 ◽  
Author(s):  
Ying Yang ◽  
Jiawu Zhou ◽  
Jing Li ◽  
Peng Xu ◽  
Yu Zhang ◽  
...  
Keyword(s):  
Wild Species ◽  
Hybrid Sterility ◽  
Aa Genome
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