scholarly journals Development of basic technologies for improvement of breeding and cultivation of Japanese gentian

2018 ◽  
Vol 68 (1) ◽  
pp. 14-24 ◽  
Author(s):  
Masahiro Nishihara ◽  
Keisuke Tasaki ◽  
Nobuhiro Sasaki ◽  
Hideyuki Takahashi
Keyword(s):  
Japanese Gentian

scholarly journals Development of Simple Sequence Repeat Markers for Identification of Japanese Gentian Cultivars

2011 ◽  
Vol 80 (4) ◽  
pp. 475-485 ◽  
Author(s):  
Yuka Sato-Ushiku ◽  
Norimoto Shimada ◽  
Misa Saito ◽  
Eri Yamada ◽  
Takashi Hikage ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
Japanese Gentian ◽  
Simple Sequence

scholarly journals A New Method with a Pair of Polarizing Filters for Quantification of Luster of Corollas of Japanese Gentian Flowers for Determination of Growth Stage

2012 ◽  
Vol 21 (4) ◽  
pp. 95-105
Author(s):  
Hiroshi Shono ◽  
Kazuhiro Serizawa ◽  
Kyoko Yamaguchi ◽  
Takayuki Ushikusa ◽  
Uzuki Matsushima ◽  
...  
Keyword(s):  
Growth Stage ◽  
New Method ◽  
Japanese Gentian

scholarly journals Inexpensive Method for Determining the Growth Stage of Cut Japanese Gentian Flowers from Far-red Images

2012 ◽  
Vol 21 (4) ◽  
pp. 85-94
Author(s):  
Hiroshi Shono ◽  
Kazuhiro Serizawa ◽  
Asami Seki ◽  
Uzuki Matsushima ◽  
Shoji Koide ◽  
...  
Keyword(s):  
Growth Stage ◽  
Inexpensive Method ◽  
Japanese Gentian

scholarly journals Isolation and characterization of the C-class MADS-box gene involved in the formation of double flowers in Japanese gentian

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Takashi Nakatsuka ◽  
Misa Saito ◽  
Eri Yamada ◽  
Kohei Fujita ◽  
Noriko Yamagishi ◽  
...  
Keyword(s):  
Mads Box ◽  
Isolation And Characterization ◽  
Japanese Gentian ◽  
Mads Box Gene

scholarly journals The Host Stomatal Density Determines Resistance to Septoria gentianae in Japanese Gentian

2019 ◽  
Vol 32 (4) ◽  
pp. 428-436 ◽  
Author(s):  
Chika Tateda ◽  
Kazue Obara ◽  
Yoshiko Abe ◽  
Reiko Sekine ◽  
Syuuichi Nekoduka ◽  
...  
Keyword(s):  
Leaf Surface ◽  
Stomatal Density ◽  
Infection Process ◽  
Adaxial Side ◽  
Density Control ◽  
Abaxial Leaf Surface ◽  
Japanese Gentian ◽  
Blight Disease ◽  
Major Factors

Plant stomata represent the main battlefield for host plants and the pathogens that enter plant tissues via stomata. Septoria spp., a group of ascomycete fungi, use host plant stomata for invasion and cause serious damage to agricultural plants. There is no evidence, however, showing the involvement of stomata in defense systems against Septoria infection. In this study, we isolated Septoria gentianae 20-35 (Sg20-35) from Gentiana triflora showing gentian leaf blight disease symptoms in the field. Establishment of an infection system using gentian plants cultured in vitro enabled us to observe the Sg20-35 infection process and estimate its virulence in several gentian cultivars or lines. Sg20-35 also entered gentian tissues via stomata and showed increased virulence in G. triflora compared with G. scabra and their interspecific hybrid. Notably, the susceptibility of gentian cultivars to Sg20-35 was associated with their stomatal density on the adaxial but not abaxial leaf surface. Treatment of EPIDERMAL PATTERNING FACTOR-LIKE 9 (EPFL9/STOMAGEN) peptides, a small secreted peptide controlling stomatal density in Arabidopsis thaliana, increased stomatal density on the adaxial side of gentian leaves as well. Consequently, treated plants showed enhanced susceptibility to Sg20-35. These results indicate that stomatal density on the adaxial leaf surface is one of the major factors determining the susceptibility of gentian cultivars to S. gentianae and suggest that stomatal density control may represent an effective strategy to confer Septoria resistance.

scholarly journals Effects of knocking out three anthocyanin modification genes on the blue pigmentation of gentian flowers

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Keisuke Tasaki ◽  
Atsumi Higuchi ◽  
Aiko Watanabe ◽  
Nobuhiro Sasaki ◽  
Masahiro Nishihara
Keyword(s):  
Genome Editing ◽  
Hydroxy Group ◽  
Plant Species ◽  
Gene Knockout ◽  
The Other ◽  
Wild Type ◽  
Japanese Gentian ◽  
Flower Pigment ◽  
Mutant Lines

Abstract Genome editing by the CRISPR/Cas9 system has recently been used to produce gene knockout lines in many plant species. We applied this system to analyze Japanese gentian plants that produce blue flowers because of the accumulation of a polyacylated anthocyanin, gentiodelphin. Mutant lines in which anthocyanin modification genes were knocked out were examined to assess the contribution of each gene to the blue pigmentation of flowers. The targeted genes encoded anthocyanin 5-O-glycosyltransferase (Gt5GT), anthocyanin 3′-O-glycosyltransferase (Gt3′GT), and anthocyanin 5/3′-aromatic acyltransferase (Gt5/3′AT). The Gt5GT knockout lines accumulated delphinidin 3G, whereas the Gt3′GT knockout lines accumulated delphinidin 3G-5CafG as the major flower pigment. Knocking out Gt5/3′AT resulted in the accumulation of delphinidin 3G-5G-3′G and delphinidin 3G-5G as the primary and secondary pigments, respectively. These results indicated the existence of two pathways mediating the modification of delphinidin 3G-5G in flowers, with one involving a glycosylation by 3′GT and the other involving an acylation by 5/3′AT. The Gt5GT, Gt3′GT, and Gt5/3′AT transformants produced pale red violet, dull pink, and pale mauve flowers, respectively, unlike the vivid blue flowers of wild-type plants. Thus, the glycosylation and subsequent acylation of the 3′-hydroxy group of the B-ring in delphinidin aglycone is essential for the development of blue gentian flowers.

scholarly journals Molecular characterization of an anthocyanin-related glutathione S-transferase gene in Japanese gentian with the CRISPR/Cas9 system

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Keisuke Tasaki ◽  
Momo Yoshida ◽  
Minori Nakajima ◽  
Atsumi Higuchi ◽  
Aiko Watanabe ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Glutathione S Transferase ◽  
Transferase Gene ◽  
Japanese Gentian
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