scholarly journals Extension of In vitro Life-span of .GAMMA.-irradiated Human Embryo Cells Accompanied by Chromosome Instability.

1998 ◽  
Vol 39 (3) ◽  
pp. 203-213 ◽  
Author(s):  
MASAO SUZUKI ◽  
ZHI YANG ◽  
KAZUSHIRO NAKANO ◽  
FUMIO YATAGAI ◽  
KEIJI SUZUKI ◽  
...  
Keyword(s):  
Life Span ◽  
Human Embryo ◽  
Chromosome Instability ◽  
Embryo Cells ◽  
Gamma Irradiated

scholarly journals The extended survival of tw5/tw5 mouse embryo cells in vitro

1990 ◽  
Vol 55 (1) ◽  
pp. 21-25 ◽  
Author(s):  
William Garside ◽  
Nina Hillman
Keyword(s):  
Life Span ◽  
Mouse Embryo ◽  
Culture Media ◽  
Mouse Embryos ◽  
Recessive Mutation ◽  
Continue Development ◽  
Embryo Cells ◽  
And Control ◽  
Extended Survival

SummaryThe tw5 haplotype is a recessive mutation which is lethal when homozygous in mouse embryos following implantation. This series of studies was undertaken to determine the effect of the tw5/tw5 genotype on embryos developing in vitro. Blastocyst embryos from + / tw5inter se matings were compared with control blastocysts obtained from matings between T/ + and + / + females and + / tw5 males for their abilities to continue development in vitro in two culture media. The data show that there are no significant differences between the percentages of experimental and control blastocyst embryos which attach and outgrow or which contain inner cell masses on any day of culture up to equivalent gestation day 21 in either media. These findings show that the life span of cells from tw5 / tw5 embryos can be extended significantly by in vitro culture.

scholarly journals Transformation in vitro of Human Embryo Cells by Human Leukemic Culture Fluid. II

1968 ◽  
Vol 44 (2) ◽  
pp. 95-98
Author(s):  
Ikuo KIMURA ◽  
Toyoro OSATO ◽  
Toshio NAGANO ◽  
Yohei ITO
Keyword(s):  
Human Embryo ◽  
Culture Fluid ◽  
Embryo Cells

Application of Transmission Electron Microscope and Scanning Electron Microscope in experimental tumor studies

1990 ◽  
Vol 48 (3) ◽  
pp. 306-307
Author(s):  
Gao Fengming
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Malignant Transformation ◽  
Transmission Electron Microscope ◽  
Experimental Tumor ◽  
Transmission Electron ◽  
Embryo Cells ◽  
Tumor Studies ◽  
Scanning Electron

Transmission electron microscope(TEM) and scanning electron microscope(SEM) were widely used in experimental tumor studies. They are useful for evaluation of cellular transformation in vitro, classification of histological types of tumors and treating effect of tumors. We have obtained some results as follows:1. Studies on the malignant transformation of mammalian cells in vitro. Syrian golden hamster embryo cells(SGHEC) were transformed in vitro by ThO2 and/or ore dust. In a few days after dust added into medium, some dust crystals were phagocytized. Two weeks later, malignant transformation took place. These cells were of different size, nuclear pleomorphism, numerous ribosomes, increasing of microvilli on cell surface with various length and thickness, and blebs and ruffles(Figs. 1,2). Myelomonocytic leukemic transformation of mouse embryo cells(MEC) was induced in vitro by 3H-TdR. Transformed cells were become round from fusiform. The number of mitochondria and endoplasmic reticulum was reduced, ribosomes and nucleoli increased, shape of nuclei irregular, microvilli increased, and blebs and ruffles appeared(Fig. 3).

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